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- 저자 : Toni HERAWAN (검색결과 2 건)
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Putri Asri Insiana,Kartikawati Noor Khomsah,Nirsatmanto Arif,Sunarti Sri,Haryjanto Liliek,Herawan Toni,Santosa Purwanto Budi,Wahyuningtyas Reni Setyo,Lestari Fajar,Rimbawanto Anto 한국산림과학회 2023 Forest Science And Technology Vol.19 No.3
Gerunggang (Cratoxylum arborescens (Vahl) Blume) is a tree native to Indonesian peatlands that has great potential as an alternative fiber-producing species for the pulp and paper industry because it is adaptable and does not have the potential to disrupt local ecosystems. The species also contains important anti-cancer compounds for the pharmaceutical industry. These industries require large quantities of raw materials to meet their needs, which cannot be met by the supply of conventional plant seedlings. Tissue culture is one of the propaga- tion biotechnologies that can be used as an alternative to provide more efficient germplasm. In this study, we developed a protocol for gerunggang propagation using tissue culture technique. The results showed that the combination of 1 mg/l 6-benzylamino purine (BAP) and 0.5 mg/l naphthalene acetic acid (NAA) in Murashige & Skoog media provided the fast- est bud break time (2 weeks before the first subculture), with the highest shoot initiation (3.0 ± 0.2 cm), and after 4 weeks of incubation, the highest auxiliary shoot elongation (7.4 ± 0.1 cm). The significant effect of this hormone concentration also increases the nodule multiplication coefficient (NMC), which is 7.5. In vitro root response on the same propagation medium showed good growth with an average root length of 4.8 ± 0.2 cm. Acclimatization of plantlets was successful under ex vitro conditions and resulted in good growth after 2 and 3 months in the greenhouse. The advantage of applying the tissue culture method to gerunggang is that it can produce about seven times more seedlings from one explant in about 4 weeks. These results will be very useful for further research in the field and require a more intensive and comprehensive study.
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Rizki ARISANDI,Koetsu Takahashi,Arif NIRSATMANTO,Sri SUNARTI,Anto RIMBAWANTO,Asri Insiana PUTRI,Noor Khomsah KARTIKAWATI,Liliek HARYJANTO,Toni HERAWAN,Fajar LESTARI,Ganis Lukmandaru 한국목재공학회 2024 목재공학 Vol.52 No.1
Swietenia mahagoni is one of the commercial timbers in Indonesia. Mahogany heartwood is an important characteristic as it relates to the natural durability and aesthetics of the wood. Lipophilic extractives are known to be involved in the heartwood formation process. Therefore, this study aims to determine the lipophilic compounds associated with heartwood formation. The n-hexane extract from sapwood and heartwood samples (1 to 5 years) was analyzed by gas chromatography-mass spectrometry. The results showed that the content of n-hexane extract ranged from 0.76% to 2.45% based on dry wood. The main group of compounds identified in the lipophilic fraction consisted of sterols (β-sitosterol, stigmasterol, campasterol, and cyclolaudenol), fatty acids (palmitic, oleic, linoleic, and stearic acid), and hydrocarbons (pentadecane, 1-octadecane, hexadecane, cyclotetracosane, cycloeicosane, and cyclooctacosane) after heartwood formation. In addition, the hydrocarbon fraction was the largest, followed by sterols, fatty acids, and 1-heneicosanol. In the radial variation, the distribution of fatty acids was greater in the sapwood than in the heartwood (4-year-old). However, the reverse pattern was found at the age of 5 years. The lipophilic fraction was generally more abundant in the heartwood compared to the sapwood, especially at 5 years of age, with much higher levels than when the heartwood was forming (4 years). These findings show that when the heartwood formation begins, the lipid composition was not fully metabolized at the beginning of heartwood formation compared to 5-year-old trees.
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