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RISS 인기검색어
- 검색키워드
- 저자 : Thomas W. Molitor (검색결과 3 건)
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Thomas W. Molitor,Jin-Ho Shin 대한수의학회 2002 Journal of Veterinary Science Vol.3 No.2
Porcine reproductive and respiratory syndromevirus (PRRSV) RNA load in sera and tissues duringacute phase of infection was evaluated using a PCR-based quantitative assay. More than 80% of infectedpigs (21/25) showed the peak level of viral RNAconcentrations in serum (up to 8.6 × 108copies/ml) atday 5 postinfection (PI),and started to clear the virusfrom the systemic circulation thereafter. Regressionanalysis using the viral RNA concentrations in seraobtained from days 5 to 14 PI showed that the viralRNA was cleared at the rate of 0.37 log reduction inthe number of PRRSV RNA copies per day. It wasestimated to be day 27 PI when the viral RNA in theserum of infected pigs becomes undetectable. Whencorrelation analysis was performed between thesystemic clearance rate and viral RNA concen-trations in tissues of 9 infected pigs obtained at day14 PI, moderately strong negative correlation wasobserved in the thymus (r = - 0.62) and brain stem (r= - 0.48), suggesting the capability of host animal toclear PRRSV from the systemic circulation appears tobe related to the viral activity in the thymus andbrain stem.
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Thomas W. Molitor,Jin-Ho Shin 대한수의학회 2002 Journal of Veterinary Science Vol.3 No.2
The capability of porcine reproductive and respiratorysyndrome virus (PRRSV) to be shed in semen forextended periods of time has been suggested to be aprincipal factor for viral transmission via insemination.In attempts to gain insights into the mechanism ofPRRSV persistence in boars, tissue distribution andsites of viral infection were investigated by in situhybridization (ISH) using digoxigenin-labeled RNAprobe and the ISH results were compared with thoseof reverse transcription-nested polymerase chainreaction (RT-nested PCR). Animals were intranasallyinoculated with 104 median tissue culture infectiousdose of PRRSV VR-2332 and tissues collected atdifferent times were examined.At day 7 postinfection,limited number of hybridization positive signals wasobserved in cells w ithin or betw een seminiferou stubules in the testis sections while relatively abundanthybridization positive signals were observed in thebrain stem and tracheobronchial lymph node.At laterdays of infection, hybridization positive signals wereobserved in cells within seminiferous tubules withmuch reduced frequency.Lack of agreement with theRT-nested PCR assay results in testis tissues obtainedat days 14, 28, and 59 postinfection suggested thatPRRSV infection in the testis may be extremelyrestricted,and may not necessarily constitute a majorviral source in semen during extended periods ofseminal shedding.
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Viral characteristics of plaque variants of porcine reproductive and respiratory syndrome virus
Park, Bong-kyun,Molitor, Thomas W.,Joo, Han-soo The Korean Society of Veterinary Science 1999 大韓獸醫學會誌 Vol.39 No.4
Plaque characteristics of porcine reproductive and respiratory syndrome (PRRS) virus isolates were examined using MARC-145 line cells. The plaque morphology of PRRS virus isolates was variable in size and heterogenic in population. Upon serial passages of the PRRS virus isolates on MARC-145 tells, heterogeneity was maintained but numbers of the large plaque size virus were increased with certain isolates. A PRRS virus isolate with variable plaque sizes was subcloned into 2 populations : small plaque ($H_S$) and large plaque ($H_L$) viruses. Growth kinetics of the subclones were then determined in MARC-145 cells, and production of the structural polypeptides was analyzed by SDS-PAGE. In a comparison of the growth kinetics, the $H_S$ virus showed higher infectivity titers during the first 48 hours but slower to reach the peak titier than $H_L$ virus did. In a nucleotide sequence comparison, differences of 4 nucleotides in open reading frames 5-6 gene were found between $H_S$ and $H_L$ viruses. Both the $H_S$ and $H_L$ clones produced 5 polypeptide bands with molecular weights of 15, 19, 26, 36 and 42 kD. The 5 bands were detected at 48 hours postinoculation (PI) with antisera to $H_L$ and another large plaque virus ($W_L$) and at 72 hours PI with $H_S$ virus antiserum. The present results demonstrate differences of biologic and molecular characteristics between the two PRRS virus plaque clones.
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