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Glasa, Miroslav,Predajna, Lukas,Soltys, Katarina,Sihelska, Nina,Nagyova, Alzbeta,Wetzel, Thierry,Sabanadzovic, Sead The Korean Society of Plant Pathology 2017 Plant Pathology Journal Vol.33 No.1
Grapevine rupestris stem pitting-associated virus (GRSPaV) is a worldwide-distributed pathogen in grapevines with a high genetic variability. Our study revealed differences in the complexity of GRSPaV population in a single host. A single-variant GRSPaV infection was detected from the SK30 grapevine plant. On the contrary, SK704 grapevine was infected by three different GRSPaV variants. Variant-specific RT-PCR detection protocols have been developed in this work to study distribution of the three different variants in the same plant during the season. This study showed their randomized distribution in the infected SK704 grapevine plant. Comparative analysis of full-length genome sequences of four Slovak GRSPaV isolates determined in this work and 14 database sequences showed that population of the virus cluster into four major phylogenetic lineages. Moreover, our analyses suggest that genetic recombination along with point mutations could play a significant role in shaping evolutionary history of GRSPaV and contributed to its extant genetic diversification.
Miroslav Glasa,Lukáš Predajňa,Katarína Šoltys,Nina Sihelská,Alžbeta Nagyová,Thierry Wetzel,Sead Sabanadzovic 한국식물병리학회 2017 Plant Pathology Journal Vol.33 No.1
Grapevine rupestris stem pitting-associated virus(GRSPaV) is a worldwide-distributed pathogen ingrapevines with a high genetic variability. Our studyrevealed differences in the complexity of GRSPaVpopulation in a single host. A single-variant GRSPaVinfection was detected from the SK30 grapevine plant. On the contrary, SK704 grapevine was infected bythree different GRSPaV variants. Variant-specific RTPCRdetection protocols have been developed in thiswork to study distribution of the three different variantsin the same plant during the season. This studyshowed their randomized distribution in the infectedSK704 grapevine plant. Comparative analysis of fulllengthgenome sequences of four Slovak GRSPaVisolates determined in this work and 14 database sequencesshowed that population of the virus clusterinto four major phylogenetic lineages. Moreover, ouranalyses suggest that genetic recombination along withpoint mutations could play a significant role in shapingevolutionary history of GRSPaV and contributedto its extant genetic diversification.