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Nina Sihelská,Zuzana Vozárová,Lukáš Predajňa,Katarína Šoltys,Martina Hudcovicová,Daniel Mihálik,Ján Kraic,Michaela Mrkvová,Otakar Kúdela,Miroslav Glasa 한국식물병리학회 2017 Plant Pathology Journal Vol.33 No.5
The complete genome sequence of a Slovak SL-1 isolate of Tomato mosaic virus (ToMV) was determined from the next generation sequencing (NGS) data, further confirming a limited sequence divergence in this tobamovirus species. Tomato genotypes Monalbo, Mobaci and Moperou, respectively carrying the susceptible tm-2 allele or the Tm-1 and Tm-2 resistant alleles, were tested for their susceptibility to ToMV SL- 1. Although the three tomato genotypes accumulated ToMV SL-1 to similar amounts as judged by semiquantitative DAS-ELISA, they showed variations in the rate of infection and symptomatology. Possible differences in the intra-isolate variability and polymorphism between viral populations propagating in these tomato genotypes were evaluated by analysis of the capsid protein (CP) encoding region. Irrespective of genotype infected, the intra-isolate haplotype structure showed the presence of the same highly dominant CP sequence and the low level of population diversity (0.08-0.19%). Our results suggest that ToMV CP encoding sequence is relatively stable in the viral population during its replication in vivo and provides further demonstration that RNA viruses may show high sequence stability, probably as a result of purifying selection.
Miroslav Glasa,Lukáš Predajňa,Katarína Šoltys,Nina Sihelská,Alžbeta Nagyová,Thierry Wetzel,Sead Sabanadzovic 한국식물병리학회 2017 Plant Pathology Journal Vol.33 No.1
Grapevine rupestris stem pitting-associated virus(GRSPaV) is a worldwide-distributed pathogen ingrapevines with a high genetic variability. Our studyrevealed differences in the complexity of GRSPaVpopulation in a single host. A single-variant GRSPaVinfection was detected from the SK30 grapevine plant. On the contrary, SK704 grapevine was infected bythree different GRSPaV variants. Variant-specific RTPCRdetection protocols have been developed in thiswork to study distribution of the three different variantsin the same plant during the season. This studyshowed their randomized distribution in the infectedSK704 grapevine plant. Comparative analysis of fulllengthgenome sequences of four Slovak GRSPaVisolates determined in this work and 14 database sequencesshowed that population of the virus clusterinto four major phylogenetic lineages. Moreover, ouranalyses suggest that genetic recombination along withpoint mutations could play a significant role in shapingevolutionary history of GRSPaV and contributedto its extant genetic diversification.