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Partial Purification of Lectin from Mycoparasitic Species of Trichoderma
Tanuja Singh,Ratul Saikia,Dilip K. Arora 한국식물병리학회 2005 Plant Pathology Journal Vol.21 No.4
Trichoderma species/isolates exhibited varied degree of agglutination on sclerotial (Sc) and hyphal (Hy) surface of Macrophomina phaseolina. The agglutination efficiencies on Sc and Hy ranged from 11 to 57%. Isolates of T. harzianum (Th) and T. viride (Tv) showed greater agglutination on Sc (23-57%) and Hy (16-47%). Different enzymes (trypsin, pepsin, proteinase k, α-chymotrypsin, lyticase and glucosidase) and inhibitors (tunicamycin,cycloheximide, brefeldin A, sodium azide,dithiothreitol and SDS) reduced the agglutination potential of conidia of Th-23/98 and Tv-25/98; however, the extent of response varied greatly in different treatments.Different fractions of Th-23/98 and Tv-25/98 exhibited haemagglutinating reaction with human blood group A, B, AB and O. Haemagglutinating activity was inhibited by different sugars and glycoproteins tested. Crude haemagglutinating protein from outer cell wall protein fraction of Th-23/98 and Tv-25/98 were eluted on Sephadex G-100 column. Initially Th-23/98 and Tv- 25/98 exhibited two peaks showing no agglutination activity; however, lectin activity was detected in the third peak. Similar to crude lectin, the purified lectin also exhibited haemagglutinating activity with different erythrocyte source. SDS-PAGE analysis of partially purified lectin revealed single band with an estimated molecular mass of 55 and 52 kDa in Th-23/98 and Tv- 25/98, respectively. Trypsin, chymotrypsin and b-1,3- glucanase totally inhibited lectin activity. Similarly,various pH also affected the haemagglutinating activity of Th-23/98 and Tv-25/98. From the present observations, it can be concluded that the recognition/attachment of mycoparasite (T. harzianum and T. viride) to the host surface (M. phaseolina) may be most likely due to lectincarbohydrate interaction.
Partial Purification of Lectin from Mycoparasitic Species of Trichoderma
Singh, Tanuja,Saikia, Ratul,Arora, Dilip K. The Korean Society of Plant Pathology 2005 Plant Pathology Journal Vol.21 No.4
Trichoderma species/isolates exhibited varied degree of agglutination on sclerotial (Sc) and hyphal (Hy) surface of Macrophomina phaseolina. The agglutination efficiencies on Sc and Hy ranged from $11\;to\;57\%$. Isolates of T. harzianum (Th) and T. viride (Tv) showed greater agglutination on Sc ($23-57\%$) and Hy ($16-47\%$). Different enzymes (trypsin, pepsin, proteinase k, a-chymotrypsin, lyticase and glucosidase) and inhibitors (tunicamycin, cycloheximide, brefeldin A, sodium azide, dithiothreitol and SDS) reduced the agglutination potential of conidia of Th-23/98 and Tv-25/98; however, the extent of response varied greatly in different treatments. Different fractions of Th-23/98 and Tv-25/98 exhibited haemagglutinating reaction with human blood group A, B, AB and O. Haemagglutinating activity was inhibited by different sugars and glycoproteins tested. Crude haemagglutinating protein from outer cell wall protein fraction of Th-23/98 and Tv-25/98 were eluted on Sephadex G-100 column. Initially Th-23/98 and Tv-25/98 exhibited two peaks showing no agglutination activity; however, lectin activity was detected in the third peak. Similar to crude lectin, the purified lectin also exhibited haemagglutinating activity with different erythrocyte source. SDS-PAGE analysis of partially purified lectin revealed single band with an estimated molecular mass of 55 and 52 kDa in Th-23/98 and Tv-25/98, respectively. Trypsin, chymotrypsin and b-1,3-glucanase totally inhibited lectin activity. Similarly, various pH also affected the haemagglutinating activity of Th-23/98 and Tv-25/98. From the present observations, it can be concluded that the recognition/attachment of mycoparasite (T. harzianum and T. viride) to the host surface (M. phaseolina) may be most likely due to lectin-carbohydrate interaction.
Kabdal Tanuja,Karakoti Himani,Upadhyay Pringal,Kumar Ravendra,Prakash Om,Dhami Anamika,Latwal Mamta,Pandey Ganesh,Srivastava R.M.,Kumar Satya,Rawat D.S. 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2
The present research aimed to analyze the nematicidal and insecticidal activity of the essential oil obtained from the fruits of Zanthoxylum armatum collected from Gumma village of Dharchula and Chandak village of Pithor agarh. After extraction via hydro distillation, the essential oils were subjected to GC–MS for their compositional analysis. A total of 10 and 13 components were identified for the Dharchula and Pithoragarh collection respectively. Linalool (32.86 %) was the major component in collection from Dharchula whereas it was totally absent in that of collection from Pithoragarh which has trans-sabinene hydrate (11.7 %) as its major component. Essential oils were tested against Meloidogyne incognita for their nematicidal activity and inhibition of egg hatching in laboratory conditions. It was found that the activity of Z. armatum from Dharchula shows high mortality of Meloidogyne incognita and more inhibition to the egg hatching. When tested for insecticidal activity against Lipaphis erysimi, collection from Dharchula presented stronger activity compared to Pithoragarh collec tion as shown by their LC 50 values 0.006 and 0.165 µL/mL respectively after 36 hrs. The in silico PASS study and molecular docking study was conducted to screen the nematicidal and insecticidal potential of the major com ponents on acetylcholinesterase receptors. Results showed good binding affinities and attributed the strongest inhibitory effect to verbenone for the target protein.
H. B. Kekre,Tanuja sarode,Shachi Natu 보안공학연구지원센터(IJSIP) 2014 International Journal of Signal Processing, Image Vol.7 No.6
In this paper a watermarking technique using hybrid wavelet transforms obtained from sinusoidal and non-sinusoidal component orthogonal transforms is proposed. Sinusoidal transform DCT and non-sinusoidal transforms Walsh, Haar and Discrete Kekre Transform are used to generate hybrid wavelet transforms namely DCT-Walsh, Walsh-DCT, DCT-Haar, Haar-DCT, DCT-DKT and DKT-DCT. Size of each component transform matrix is varied suitably from 4, 8, 16, 32, and 64 to generate hybrid wavelet transform matrix for host and watermark. The best size combination is further applied column wise and row wise to host and watermark and to embed the watermark middle frequency regions of host is selected. Embedding is first done without sorting the hybrid wavelet transform coefficients of host and watermark and then sorting is applied to observe the difference in the achieved robustness. Performance of proposed technique is evaluated against various attacks to decide whether sinusoidal transform when used as base transform matrix or local transform matrix is more robust.
Color Image Compression using DKT-DCT Hybrid Wavelet Transform in Various Color Spaces
H.B. Kekre,Tanuja Sarode,Prachi Natu 보안공학연구지원센터 2014 International Journal of Signal Processing, Image Vol.7 No.5
This paper proposes image compression in different color spaces using hybrid wavelet transform. To generate hybrid wavelet transform Discrete Kekre transform (DKT) and Discrete Cosine transform (DCT) are selected as component transforms. Due to high energy compaction property, DCT is selected as local component transform that contributes to local features of an image. Hybrid wavelet transform extracts features of both the component transforms and hence gives less error and better image quality. Component transforms of different sizes are selected to generate hybrid wavelet of size 256x256 and applied on images. In RGB color space 16-16 combination i.e. hybrid wavelet generated using DKT 16x16 and DCT 16x16 gives least error than other combinations like 8-32, 32-8 and 64-4. RMSE, MAE, AFCPV and Structural Similarity Index (SSIM) are the error metrics used to measure reconstructed image quality. Different color spaces have been used to observe the performance of this hybrid wavelet transform. In KLUV color space minimum RMSE and MAE is observed than RGB, YUV, YCbCr, XYZ and YIQ color space. Whereas RGB color space gives lowest AFCPV than other color spaces using 16-16 component size. Hence SSIM is used to eliminate this inconsistency in these traditional error metrics. KLUV color space gives highest SSIM 0.998 which is closest to maximum one proving it as a better choice than other color spaces.
Uniqueness and Value-Sharing of Meromorphic Functions
Waghamore, Harina P.,Tanuja, A. Department of Mathematics 2014 Kyungpook mathematical journal Vol.54 No.4
In this paper, we prove two uniqueness theorem on meromorphic functions sharing one value which generalize a recent result of R. S. Dyavanal [2], and on the other hand, we relax the nature of sharing value from CM to IM.
Cayley signed graphs associated with Abelian groups
Pranjali,Amit Kumar,Tanuja Yadav 한국전산응용수학회 2022 Journal of applied mathematics & informatics Vol.40 No.3
The aim of author's in this paper is to study the Cayley graph in the realm of signed graph. Moreover, we have characterized generating sets and finite abelian groups that corresponds to balanced Cayley signed graphs. The notion of Cayley signed graph has been demonstrated with the ample number of examples.
Abhishek Mahajan,Amar Udare,Tanuja Shet,Shashikant Juvekar,Meenakshi Thakur 대한영상의학회 2013 Korean Journal of Radiology Vol.14 No.4
Metastases to intramammary nodes have been shown to be an independent predictor of poor outcome in patients with breast cancer, such as axillary lymph node metastases. The detection and accurate characterization of these nodes preoperatively is thus crucial for the staging and planning of treatment for breast carcinoma, particularly in cases with axillary lymph node negative disease as it upgrades the disease staging. We herein report the first case where we detected an intra-mammary node on specimen MRI after the primary pathological gross specimen evaluation failed to detect the node.