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      • KCI등재후보

        Integrated analysis of transcriptome and milk metagenome in subclinical mastitic and healthy cows

        Zhang Jinning,Liu Xueqin,Usman Tahir,Tang Yongjie,Mi Siyuan,Li Wenlong,Yang Mengyou,Yu Ying 아세아·태평양축산학회 2024 Animal Bioscience Vol.37 No.4

        Objective: Abnormally increased somatic cell counts (SCCs) in milk is usually a sign of bovine subclinical mastitis. Mutual interaction between the host and its associated microbiota plays an important role in developing such diseases. The main objective of this study was to explore the difference between cows with elevated SCCs and healthy cattle from the perspective of host-microbe interplay. Methods: A total of 31 milk samples and 23 bovine peripheral blood samples were collected from Holstein dairy cattle to conduct an integrated analysis of transcriptomic and metagenomics. Results: The results showed that Ralstonia and Sphingomonas were enriched in cows with subclinical mastitis. The relative abundance of the two bacteria was positively correlated with the expression level of bovine transcobalamin 1 and uridine phosphorylase 1 encoding gene. Moreover, functional analysis revealed a distinct alternation in some important microbial biological processes. Conclusion: These results reveal the relative abundance of Ralstonia and Sphingomonas other than common mastitis-causing pathogens varied from healthy cows to those with subclinical mastitis and might be associated with elevated SCCs. Potential association was observed between bovine milk microbiota composition and the transcriptional pattern of some genes, thus providing new insights to understand homeostasis of bovine udder.

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        Postpartum depression and its correlates in middle‐class women in Hunan, China

        Guan Ziyao,Bai Wenhui,Qin Chunxiang,Sun Mei,Bai Xiaoling,Tang Siyuan 한국사회복지학회 2020 Asian Social Work and Policy Review Vol.14 No.2

        Postpartum depression (PPD) has a negative impact on both infants and women. The middle-class group is rapidly expanding with increased but unmet psychological needs. To determine the risk and its correlates of PPD in middle-class women in Hunan, China. A sample of 306 middle-class puerperae from obstetric wards of a large general hospital was enrolled in the study. Our results showed 55.6% middleclass women were suspected PPD. Related risk factors included a history of complications, pregnancy stress, subjective support, and interaction effect between objective support and negative coping. The protective factors included objective support and an interaction effect between subjective support and negative coping. Early assessment and individualized guidance in terms of social support and coping to identify and prevent PPD in this population are required.

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