Effects of Long-Term Fertilizer Practices on Rhizosphere Soil Autotrophic CO₂-Fixing Bacteria under Double Rice Ecosystem in Southern China

( Haiming Tang ),( Li Wen ),( Lihong Shi ),( Chao Li ),( Kaikai Cheng ),( Weiyan Li ),( Xiaoping Xiao ) 한국미생물생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.10

Soil autotrophic bacterial communities play a significant role in the soil carbon (C) cycle in paddy fields, but little is known about how rhizosphere soil microorganisms respond to different long-term (35 years) fertilization practices under double rice cropping ecosystems in southern China. Here, we investigated the variation characteristics of rhizosphere soil RubisCO gene cbbL in the double rice ecosystems of in southern China where such fertilization practices are used. For this experiment we set up the following fertilizer regime: without any fertilizer input as a control (CK), inorganic fertilizer (MF), straw returning (RF), and organic and inorganic fertilizer (OM). We found that abundances of cbbL, 16S rRNA genes and RubisCO activity in rhizosphere soil with OM, RF and MF treatments were significantly higher than that of CK treatment. The abundances of cbbL and 16S rRNA genes in rhizosphere soil with OM treatment were 5.46 and 3.64 times higher than that of CK treatment, respectively. Rhizosphere soil RubisCO activity with OM and RF treatments increased by 50.56 and 45.22%, compared to CK treatment. Shannon and Chao1 indices for rhizosphere soil cbbL libraries with RF and OM treatments increased by 44.28, 28.56, 29.60, and 23.13% compared to CK treatment. Rhizosphere soil cbbL sequences with MF, RF and OM treatments mainly belonged to Variovorax paradoxus, uncultured proteobacterium, Ralstonia pickettii, Thermononospora curvata, and Azoarcus sp.KH33C. Meanwhile, cbbL-carrying bacterial composition was obviously influenced by soil bulk density, rhizosphere soil dissolved organic C, soil organic C, and microbial biomass C contents. Fertilizer practices were the principal factor influencing rhizosphere soil cbbL-carrying bacterial communities. These results showed that rhizosphere soil autotrophic bacterial communities were significantly changed under conditions of different long-term fertilization practices Therefore, increasing rhizosphere soil autotrophic bacteria community with crop residue and organic manure practices was found to be beneficial for management of double rice ecosystems in southern China.

Resource recovery and harmless treatment of waste oil-in-water drilling fluid

Chao Tang,Shui Xiang Xie 대한환경공학회 2017 Environmental Engineering Research Vol.22 No.3

Destablization and demulsification is a difficult task for the treatment of waste oil-in-water drilling fluid because of its “three-high” characteristics: emulsification, stabilization and oiliness. At present, China is short for effective treating technology, which restricts cleaner production in oilfield. This paper focused on technical difficulties of waste oil-in-water drilling fluid treatment in JiDong oilfield of China, adopting physical-chemical collaboration demulsification technology to deal with waste oil-in-water drilling fluid. After oil-water-solid three-phase separation, the oil recovery rate is up to 90% and the recycled oil can be reused for preparation of new drilling fluid. Meanwhile, harmless treatment of wastewater and sludge from waste oil-in-water drilling fluid after oil recycling was studied. The results showed that wastewater after treated was clean, contents of chemical oxygen demand and oil decreased from 993 mg/L and 21,800 mg/L to 89 mg/L and 3.6 mg/L respectively, which can meet the requirements of grade one of “The National Integrated Wastewater Discharge Standard” (GB8978); The pollutants in the sludge after harmless treatment are decreased below the national standard, which achieved the goal of resource recovery and harmless treatment on waste oil-in-water drilling fluid.

Shear behavior of reinforced HPC beams made of a low cement content without shear reinforcements

Chao-Wei Tang,Yu-Ping Chen,How-Ji Chen,Chung-Ho Huang,Tsang-Hao Liu 사단법인 한국계산역학회 2013 Computers and Concrete, An International Journal Vol.11 No.1

High-performance concrete (HPC) usually has higher paste and lower coarse aggregate volumes than normal concrete. The lower aggregate content of HPC can affect the shear capacity of concrete members due to the formation of smooth fractured surfaces and the subsequent development of weak interface shear transfer. Therefore, an experimental investigation was conducted to study the shear strength and cracking behavior of full-scale reinforced beams made with low-cement-content high-performance concrete (LcHPC) as well as conventional HPC. A total of fourteen flexural reinforced concrete (RC) beams without shear reinforcements were tested under a two-point load until shear failure occurred. The primary design variables included the cement content, the shear span to effective depth ratio (a/d), and the tensile steel ratio (ρw). The results indicate that LcHPC beams show comparable behaviors in crack and ultimate shear strength as compared with conventional HPC beams. Overall, the shear strength of LcHPC beams was found to be larger than that of corresponding HPC beams, particularly for an a/d value of 1.5. In addition, the crack and ultimate shear strength increased as a/d decreased or ρw increased for both LcHPC beams and HPC beams. This investigation established that LcHPC is recommendable for structural concrete applications.

Investigation on correlation between pulse velocity and compressive strength of concrete using ANNs

Chao-Wei Tang,Yiching Lin,Shih-Fang Kuo 한국계산역학회 2007 Computers and Concrete, An International Journal Vol.4 No.6

The ultrasonic pulse velocity method has been widely used to evaluate the quality of concrete and assess the structural integrity of concrete structures. But its use for predicting strength is still limited since there are many variables affecting the relationship between strength and pulse velocity of concrete. This study is focused on establishing a complicated correlation between known input data, such as pulse velocity and mixture proportions of concrete, and a certain output (compressive strength of concrete) using artificial neural networks (ANN). In addition, the results predicted by the developed multilayer perceptrons (MLP) networks are compared with those by conventional regression analysis. The result shows that the correlation between pulse velocity and compressive strength of concrete at various ages can be well established by using ANN and the accuracy of the estimates depends on the quality of the information used to train the network. Moreover, compared with the conventional approach, the proposed method gives a better prediction, both in terms of coefficients of determination and root-mean-square error.

Hydration properties of cement pastes containing high-volume mineral admixtures

Chao-Wei Tang 사단법인 한국계산역학회 2010 Computers and Concrete, An International Journal Vol.7 No.1

This research aimed to investigate the influence of high-volume mineral admixtures (MAs), i.e., fly ash and slag, on the hydration characteristics and microstructures of cement pastes. Degree of cement hydration was quantified by the loss-on-ignition technique and degree of pozzolanic reaction was determined by a selective dissolution method. The influence of MAs on the pore structure of paste was measured by mercury intrusion porosimetry. The results showed that the hydration properties of the blended pastes were a function of water to binder ratio, cement replacement level by MAs, and curing age. Pastes containing fly ash exhibited strongly reduced early strength, especially for mix with 45% fly ash. Moreover, at a similar cement replacement level, slag incorporated cement paste showed higher degrees of cement hydration and pozzolanic reaction than that of fly ash incorporated cement paste. Thus, the present study demonstrates that high substitution rates of slag for cement result in better effects on the short- and long-term hydration properties of cement pastes.

Strength degeneracy of LWAC and flexural behavior of LWAC members after fire

Chao-Wei Tang 사단법인 한국계산역학회 2017 Computers and Concrete, An International Journal Vol.20 No.2

The characteristics of lightweight aggregate (LWA) with a low specific gravity and high water absorption will significantly change the properties of lightweight aggregate concrete (LWAC). This study aimed at exploring the effect of presoaking degree of LWA on the strength degeneracy of LWAC and flexural behavior of LWAC members exposed to elevated temperatures. The residual mechanical properties of the LWAC subjected to elevated temperatures were first conducted. Then, the residual load tests of LWAC members (beams and slabs) after exposure to elevated temperatures were carried out. The test results showed that with increasing temperature, the decreasing trend of elastic modulus for LWAC was considerably more serious than the compressive strength. Besides, the presoaking degree of LWA had a significant influence on the residual compressive strength and elastic modulus for LWAC after exposure to 800C. Moreover, owing to different types of heating, the residual load bearing capacity of the slab specimens were significantly different from those of the beam specimens.

Local bond-slip behavior of medium and high strength fiber reinforced concrete after exposure to high temperatures

Tang, Chao-Wei Techno-Press 2018 Structural Engineering and Mechanics, An Int'l Jou Vol.66 No.4

This study aims to investigate the influence of individual and hybrid fiber on the local bond-slip behavior of medium and high strength concrete after exposure to different high temperatures. Tests were conducted on local pullout specimens (150 mm cubes) with a reinforcing bar embedded in the center section. The embedment lengths in the pullout specimens were three times the bar diameter. The parameters investigated include concrete type (control group: ordinary concrete; experimental group: fiber concrete), concrete strength, fiber type and targeted temperature. The test results showed that the ultimate bond stress in the local bond stress versus slip curve of the high strength fiber reinforced concrete was higher than that of the medium strength fiber reinforced concrete. In addition, the use of hybrid combinations of steel fiber and polypropylene fiber can enhance the residual bond strength ratio of high strength concrete.

Local bond-slip behavior of medium and high strength fiber reinforced concrete after exposure to high temperatures

Chao-Wei Tang 국제구조공학회 2018 Structural Engineering and Mechanics, An Int'l Jou Vol.66 No.4

This study aims to investigate the influence of individual and hybrid fiber on the local bond–slip behavior of medium and high strength concrete after exposure to different high temperatures. Tests were conducted on local pullout specimens (150 mm cubes) with a reinforcing bar embedded in the center section. The embedment lengths in the pullout specimens were three times the bar diameter. The parameters investigated include concrete type (control group: ordinary concrete; experimental group: fiber concrete), concrete strength, fiber type and targeted temperature. The test results showed that the ultimate bond stress in the local bond stress versus slip curve of the high strength fiber reinforced concrete was higher than that of the medium strength fiber reinforced concrete. In addition, the use of hybrid combinations of steel fiber and polypropylene fiber can enhance the residual bond strength ratio of high strength concrete.

Fire resistance of high strength concrete filled steel tubular columns under combined temperature and loading

Chao-Wei Tang 국제구조공학회 2018 Steel and Composite Structures, An International J Vol.27 No.2

In recent years, concrete-filled box or tubular columns have been commonly used in high-rise buildings. However, a number of fire test results show that there are significant differences between high strength concrete (HSC) and normal strength concrete (NSC) after being subjected to high temperatures. Therefore, this paper presents an investigation on the fire resistance of HSC filled steel tubular columns (CFTCs) under combined temperature and loading. Two groups of full-size specimens were fabricated to consider the effect of type of concrete infilling (plain and reinforced) and the load level on the fire resistance of CFTCs. Prior to fire test, a constant compressive load (i.e., load level for fire design) was applied to the column specimens. Thermal load was then applied on the column specimens in form of ISO 834 standard fire curve in a large-scale laboratory furnace until the set experiment termination condition was reached. The results demonstrate that the higher the axial load level, the worse the fire resistance. Moreover, in the bar-reinforced concrete-filled steel tubular columns, the presence of rebars not only decreased the spread of cracks and the sudden loss of strength, but also contributed to the load-carrying capacity of the concrete core.

Effect of presoaking degree of lightweight aggregate on the properties of lightweight aggregate concrete

Chao-Wei Tang 사단법인 한국계산역학회 2017 Computers and Concrete, An International Journal Vol.19 No.1

This study aimed at exploring the effect of presoaking degree of lightweight aggregate (LWA) on the fresh and hardened properties of concrete. Two series (i.e., Series A and Series B) of concrete mixes that were made of LWA with different moisture states were prepared. The presoaking degree of LWA was divided into three types: oven dry state, 1 hour prewetted and 24 hours prewetted. For the Series A, the water content of the lightweight aggregate concrete (LWAC) mixes was adjusted in accordance with the moisture condition of the LWA. Whereas the amount of water added in the Series B mixes was deliberately not adjusted for the moisture condition of the LWA. Slump test, mechanical tests, interfacial transition zone microscopical tests and thermal conductivity test were carried out on the specimens of different concretes and compared with control normal-weight aggregate concretes. The test results showed that the effect of mixing water absorption by LWA with different moisture states was reflected in the fresh concrete as the loss of mixture workability, while in the hardened concrete as the increase of its strength. With the use of oven-dried LWA, the effect of reduction of water-cement ratio was more significant, and thus the microstructure of the ITZ was more compact.