http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Kim, Hee Taek,Khang, Tae Uk,Baritugo, Kei-Anne,Hyun, Sung Min,Kang, Kyoung Hee,Jung, Sol Hee,Song, Bong Keun,Park, Kyungmoon,Oh, Min-Kyu,Kim, Gi Bae,Kim, Hyun Uk,Lee, Sang Yup,Park, Si Jae,Joo, Jeong Elsevier 2019 Metabolic engineering Vol.51 No.-
<P><B>Abstract</B></P> <P> <I>Corynebacterium glutamicum</I> was metabolically engineered for the production of glutaric acid, a C5 dicarboxylic acid that can be used as platform building block chemical for nylons and plasticizers. <I>C. glutamicum gabT</I> and <I>gabD</I> genes and <I>Pseudomonas putida davT</I> and <I>davD</I> genes encoding 5-aminovalerate transaminase and glutarate semialdehyde dehydrogenase, respectively, were examined in <I>C. glutamicum</I> for the construction of a glutaric acid biosynthesis pathway along with <I>P. putida davB</I> and <I>davA</I> genes encoding lysine 2-monooxygenase and delta-aminovaleramidase, respectively. The glutaric acid biosynthesis pathway constructed in recombinant <I>C. glutamicum</I> was engineered by examining strong synthetic promoters P<SUB>H30</SUB> and P<SUB>H36</SUB>, <I>C. glutamicum</I> codon-optimized <I>davTDBA</I> genes, and modification of <I>davB</I> gene with an N-terminal His<SUB>6</SUB>-tag to improve the production of glutaric acid. It was found that use of N-terminal His<SUB>6</SUB>-tagged DavB was most suitable for the production of glutaric acid from glucose. Fed-batch fermentation using the final engineered <I>C. glutamicum</I> H30_GA<SUB>His</SUB> strain, expressing <I>davTDA</I> genes along with <I>davB</I> fused with His<SUB>6</SUB>-tag at N-terminus could produce 24.5 g/L of glutaric acid with low accumulation of <SMALL>L</SMALL>-lysine (1.7 g/L), wherein 5-AVA accumulation was not observed during fermentation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>C. glutamicum</I> strains were metabolically engineered for the production of glutaric acid. </LI> <LI> Optimization of glutaric acid biosynthesis pathway could enhance glutaric acid production. </LI> <LI> Recombinant <I>C. glutamicum</I> strains efficiently produced glutaric acid from glucose. </LI> <LI> Fed-batch culture of recombinant <I>C. glutamicum</I> H30_GA<SUB>His</SUB> supported high-level production of glutaric acid from glucose. </LI> </UL> </P>