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種球의 低溫處理 및 培地의 組成이 마늘의 캘러스增殖 및 幼植物體 分化에 미치는 영향
閔成蘭,李殷模,羅相煜,盧泰弘,李永馥 충남대학교부설 생명공학연구소 1992 생물공학연구지 Vol.2 No.-
Effects of low temperature treatment and medium composition on callus proliferation and shoot differentiation of garlic seed bulbs were investigated. The calli derived from shoot-tip explants of bulbs which had been stored at 4℃ for 2 months were proliferated 1.9 times more vigorously by fresh weight on callus induction medium and regenerated 11 times more efficiently in number on shoot differentiation medium supplemented with 2 ㎎/L kinetin and 0.1 ㎎/L NAA than the calli from those at room temperature. Modified MS media containing KNO_3 and (NH_4)_2SO_4 of 40 mM each or containing KNO_3, (NH_4)_2SO_4, and KCI of 10 mM each were more effective than the original MS in callus proliferation and shoot differentiation. We believe that this culture system can be used for the mass production of virus-free stocks garlic.