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( Vu Thi Thu Hang ),( Dinh Thi Quyen ),( Thi Tuyet Dao ),( Sy Le Thanh Nguyen ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.3
A novel gene coding for an endo-β-1,4-mannanase (manA) from Bacillus subtilis strain G1 was cloned and overexpressed in P. pastoris GS115, and the enzyme was purified and characterized. The manA gene consisted of an open reading frame of 1,092 nucleotides, encoding a 364-aa protein, with a predicted molecular mass of 41 kDa. The β-mannanase showed an identity of 90.2-92.9% (≤95%) with the corresponding amino acid sequences from B. subtilis strains deposited in GenBank. The purified β- mannanase was a monomeric protein on SDS-PAGE with a specific activity of 2,718 U/mg and identified by MALDITOF mass spectrometry. The recombinant β-mannanase had an optimum temperature of 45oC and optimum pH of 6.5. The enzyme was stable at temperatures up to 50oC (for 8 h) and in the pH range of 5-9. EDTA and most tested metal ions showed a slightly to an obviously inhibitory effect on enzyme activity, whereas metal ions (Hg2+, Pb2+, and Co2+) substantially inhibited the recombinant β-mannanase. The chemical additives including detergents (Triton X- 100, Tween 20, and SDS) and organic solvents (methanol, ethanol, n-butanol, and acetone) decreased the enzyme activity, and especially no enzyme activity was observed by addition of SDS at the concentrations of 0.25-1.0% (w/v) or n-butanol at the concentrations of 20-30% (v/v). These results suggested that the β-mannanase expressed in P. pastoris could potentially be used as an additive in the feed for monogastric animals.
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Tran, Thu Ngan,Le, Vu Anh,Nguyen, Thi Tuyet My,Nguyen, Ngoc Bich Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.no.sup1
Printing of pictorial health warnings (PHWs) on cigarette packages became obligatory by the Vietnam Law on Prevention and Control of Tobacco Harm in May 2013. Literature from high-income countries suggests that PHWs motivate smokers to quit smoking although their long-term effects have been questioned due to reduction of impact over time. This study aimed to assess the salience of PHWs and smokers' reactions towards PHWs over time. In May 2014 and May 2015, a cross-sectional questionnaire-based household survey was administered to respectively 1,462 and 1,509 Vietnamese male smokers aged 18 to 35. The result showed that salience of the PHWs 2 years after the implementation was higher than at the point of 1 year after the implementation. The proportion of respondents who tried to avoid noting the PHWs was reduced from 35% in wave 1 to 23% in wave 2. However, "Tried to avoid looking/thinking about the PHWs" increased 1.5 times the odds of presenting quit intention compared to those respondents who did not try to avoid looking/thinking about the PHWs (OR=1.5; 95%CI: 1.2-2.0). In conclusion, avoidance regarding PHWs may not work as a barrier when aiming at a higher level of quit intention. Salience of the PHWs may increase in the period shortly after their introduction onto packs but can be expected to decrease with time. In other words, it might be advisable to change or renew PHWs after a period of implementation to maintain their beneficial effects.
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INVESTIGATION OF SHAPE CONTROLLED SILVER NANOPLATES BY A SIMPLE CHEMICAL REDUCTION METHOD
ERIC FRIBOURG-BLANC,DUNG THI MY DANG,TUYET THI THU LE,CHINH DUNG TRINH,THANH THI NU THANH,CHIEN MAU DANG 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2013 NANO Vol.8 No.3
This paper discusses the function of hydrogen peroxide and trisodium citrate (TSC) in the synthesis of silver (Ag) nanoplates through a simple chemical reduction method in ambient conditions. By this method, high purity Ag nanoplates were successfully generated (up to 100%). It was found that the amounts of hydrogen peroxide and TSC added to the solution are key to controlling the shape of Ag nanoparticles from spherical nanoparticles to hexagonal nanoplates and triangular nanoplates, depending on the Ag-to-hydrogen peroxide ratio and the Ag-to-TSC ratio used. This unique shape evolution process was carefully followed by a combination of ultraviolet-visible (UV-Vis) spectroscopy and transmission electron microscopy (TEM). The mean edge length of the triangular nanoplates varies from 65 nm to 100 nm. Polyvinyl pyrrolidone (PVP) is shown to increase the in-plane dimensions of the nanoplates as its relative concentration to Ag increases.
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Tobacco Control Policies in Vietnam: Review on MPOWER Implementation Progress and Challenges
Hoang, Van Minh,Tran, Thu Ngan,Vu, Quynh Mai,Nguyen, Thi Tuyet My,Le, Hong Chung,Vu, Duy Kien,Tran, Tuan Anh,Nguyen, Bao Ngoc,Vu, Van Giap,Nguyen, Manh Cuong,Pham, Duc Manh,Kim, Bao Giang Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.no.sup1
In Vietnam, the WHO Framework Convention on Tobacco Control (WHO FCTC) took effect in March 2005 while MPOWER has been implemented since 2008. This paper describes the progress and challenges of implementation of the MPOWER package in Vietnam. We can report that, in term of monitoring, Vietnam is very active in the Global Tobacco Surveillance System, completing two rounds of the Global Adult Tobacco Survey (GATS) and three rounds of the Global Youth Tobacco Survey (GYTS). To protect people from tobacco smoke, Vietnam has issued and enforced a law requiring comprehensive smoking bans at workplaces and public places since 2013. Tobacco advertising and promotion are also prohibited with the exception of points of sale displays of tobacco products. Violations come in the form of promotion girls, corporate social responsibility activities from tobacco manufacturers and packages displayed by retail vendors. Vietnam is one of the 77 countries that require pictorial health warnings to be printed on cigarette packages to warn about the danger of tobacco and the warnings have been implemented effectively. Cigarette tax is 70% of factory price which is equal to less than 45% of retail price and much lower than the recommendation of WHO. However, Vietnam is one of the very few countries that require manufacturers and importers to make "compulsory contributions" at 1-2% of the factory price of cigarettes sold in Vietnam for the establishment of a Tobacco Control Fund (TCF). The TCF is being operated well. In 2015, 67 units of 63 provinces/cities, 22 ministries and political-social organizations and 6 hospitals received funding from TCF to implement a wide range of tobacco control activities. Cessation services have been starting with a a toll-free quit-line but need to be further strengthened. In conclusion, Vietnam has constantly put efforts into the tobacco control field with high commitment from the government, scientists and activists. Though several remarkable achievements have been gained, many challenges remain. To overcome those challenges, implementation strategies that take into account the contextual factors and social determinants of tobacco use in Vietnam are needed.
ScienceON
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