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Inhibition of ethylene biosynthesis enhances embryogenesis of cultured microspores of Brassica napus
Benoıˆt Leroux,Nathalie Carmoy,Delphine Giraudet,Philippe Potin,Franc¸ois Larher,Manuelle Bodin 한국식물생명공학회 2009 Plant biotechnology reports Vol.3 No.4
Procedures that induce microspore embryogenesis have been described for a range of Brassica species, but embryo yield remains low for a number of genotypes. We have carried out experiments with the microspores from a weakly responsive line of B. napus to determine the culture conditions that optimize their in vitro embryogenesis by treating them with effectors of ethylene synthesis and action. The results revealed that isolated microspores subjected to an initial heat stress in a medium supplemented with inhibitors of ethylene synthesis such as AVG and CoCl2 exhibited significantly increased embryo yields. This suggested that regulatory effects are exerted by the ethylene produced by the isolated microspores on the early processes of gametogenesis. As a consequence, treatment of microspores with SAM, an ethylene synthesis precursor, or with the ethylene-releasing agent ethephon, led to decreases in embryo yield. A special response to ethylene during the early stages of microspore development was finally shown to occur through experiments where isolated microspores were treated for increasing periods of time with CoCl2. Collectively, our data demonstrated that the induction of embryogenesis induced by heat stress can be enhanced by inhibitors of ethylene biosynthesis. Procedures that induce microspore embryogenesis have been described for a range of Brassica species, but embryo yield remains low for a number of genotypes. We have carried out experiments with the microspores from a weakly responsive line of B. napus to determine the culture conditions that optimize their in vitro embryogenesis by treating them with effectors of ethylene synthesis and action. The results revealed that isolated microspores subjected to an initial heat stress in a medium supplemented with inhibitors of ethylene synthesis such as AVG and CoCl2 exhibited significantly increased embryo yields. This suggested that regulatory effects are exerted by the ethylene produced by the isolated microspores on the early processes of gametogenesis. As a consequence, treatment of microspores with SAM, an ethylene synthesis precursor, or with the ethylene-releasing agent ethephon, led to decreases in embryo yield. A special response to ethylene during the early stages of microspore development was finally shown to occur through experiments where isolated microspores were treated for increasing periods of time with CoCl2. Collectively, our data demonstrated that the induction of embryogenesis induced by heat stress can be enhanced by inhibitors of ethylene biosynthesis.
Color accuracy of imaging using color filters
Boher, P.,Leroux, T.,Patton, V. Collomb,Bignon, T. The Korean Infomation Display Society 2012 Journal of information display Vol.13 No.1
In this paper, the problem concerning the color accuracy of imaging systems using color filters is examined. It is shown that the only solution to the problem is to build systems with the spectral response matching the CIE curves as closely as possible. If the spectral response does not closely match the CIE curves, it was demonstrated that calibration cannot solve the problem and will result in very unstable colorimeters. A practical solution that uses telecentric lenses on the sensor side in addition to dedicated color filters for each CCD detector is presented. For systems that closely match the CIE curves, an innovative method of improving the color accuracy based on the precise measurement of the spectral response is presented. The small discrepancies in the spectral response with regard to the CIE curves are corrected in different ways during the measurements. Finally, it is shown that the tristimulus calibration that is used for display measurement is very unstable for systems without CIE matching and is much more stable with systems that closely match the CIE curves.
Color accuracy of imaging using color filters
P. Boher,T. Leroux,V. Collomb Patton,T. Bignon 한국정보디스플레이학회 2012 Journal of information display Vol.13 No.1
In this paper, the problem concerning the color accuracy of imaging systems using color filters is examined. It is shown that the only solution to the problem is to build systems with the spectral response matching the CIE curves as closely as possible. If the spectral response does not closely match the CIE curves, it was demonstrated that calibration cannot solve the problem and will result in very unstable colorimeters. A practical solution that uses telecentric lenses on the sensor side in addition to dedicated color filters for each CCD detector is presented. For systems that closely match the CIE curves, an innovative method of improving the color accuracy based on the precise measurement of the spectral response is presented. The small discrepancies in the spectral response with regard to the CIE curves are corrected in different ways during the measurements. Finally, it is shown that the tristimulus calibration that is used for display measurement is very unstable for systems without CIE matching and is much more stable with systems that closely match the CIE curves.
Viewing angle measurements on curved displays
P. Boher,T. Leroux,V. Collomb Patton,T. Bignon,P. Blanc 한국정보디스플레이학회 2015 Journal of information display Vol.16 No.4
The simple fact that a curved display is no more flat induces new visual effects that must be studied specifically. Display viewing angle, generally referring to variation of emissive properties (luminance and color) versus viewing direction is generally affected by the curvature and must be measure accurately. In the following article, the angular distortion of a viewing angle measurement made on a curved display when using a standard viewing angle measurement system (goniometer or Fourier optics) is expressed versus spot size D, radius of curvature R and defocus H. The limits of validity of viewing angle measurements on a curved display are deduced. If the ratio D/R of the display is below 1%, the measurement errors in the angles cannot exceed 1° for all the viewing angles for a system at focus, and the accuracy of the measurements remains excellent. Out of focus, there is also no impact of the angular accuracy if the ratio H/R is below 1%. The simulations are compared to real measurements made across flat or bent brightness enhancement film and on a curved organic light-emitting diode cell phone display.