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Lim, Sunny,Ryu, Jewon,Shin, Jin-Ae,Shin, Min-Jung,Ahn, Yeong Ki,Kim, Jae Joong,Han, Ki Hoon Ovid Technologies Wolters Kluwer -American Heart A 2009 Arteriosclerosis, thrombosis, and vascular biology Vol.29 No.12
<P>OBJECTIVE: The serum level of tumor necrosis factor-alpha (TNF-alpha) is in the picomolar range under inflammatory conditions. We investigated whether these picomolar levels of TNF-alpha directly modulate the functional activities of circulating monocytes. METHODS AND RESULTS: In THP-1 monocytes treated with TNF-alpha (1 to 100 pmol/L/30 minutes), cytosolic RhoA small GTPase rapidly translocated to the plasma membrane via functionally active ezrin/radixin/moesin (ERM) complex, a cytoskeletal linker, and subsequent actin polymerization through NF-kappaB activation. The threonine phosphorylation of ERM was accomplished by the activation of TNF receptor type I (TNFRI) and signaling pathways involving PI3K and an atypical PKC; ie, PKCzeta. The TNF-alpha-treated monocytes (10 pmol/L) displayed more potent and prolonged generation of GTP-bound RhoA in response to secondary stimulation with RhoA-activating monocyte chemoattractant protein-1 (MCP-1). Clearly, human circulating monocytes preconditioned by 10 pmol/L TNF-alpha augmented MCP-1-mediated chemotaxis and firm adhesion on VCAM-1 and ICAM-1 in vitro and ex vivo. The elevation of serum TNF-alpha (>5 pmol/L within 16 hours), which was introduced by intraperitoneal injection of mouse-specific TNF-alpha to C57/BL6 mice, enhanced the number of CD80+ monocytes transmigrating to the JE/MCP-1-injected intraperitoneal space. CONCLUSIONS: Picomolar concentrations of TNF-alpha in the bloodstream may prime the RhoA-dependent activities of circulating monocytes to enhance recruitment to active inflammatory foci.</P>
WiFi 기반 스마트폰 어플리케이션을 이용한 전기자동차 충전제어시스템
노선희(Sunny Ro),이경중(Kyung-Jung Lee),기영훈(Young-Hun Ki),안현식(Hyun-Sik Ahn) 대한전기학회 2013 전기학회논문지 Vol.62 No.8
In this paper, we propose a smartphone application based on a wireless fidelity(WiFi) in order to control the charging of electric vehicle(EV) and monitor the charging status together with the vehicle history information. The driver obtains much information on vehicle status through a smartphone application which communicates with the electric vehicle supply equipment(EVSE) management server while the EV also communicates with the EVSE for the authentification through controller area network(CAN). We also implement the simulator for the EV charging control system to verify the functions of the proposed application where the simulator consists of an EV model, an EVSE, and a smartphone. It is shown by the simulator that the proposed smartphone application allows the driver to control and to monitor the charging process of an EV conveniently and, moreover, it can provide the driver with vehicle information stored in the EVSE management server.