Single‐Molecule Four‐Color FRET

Lee ,, Jinwoo,Lee ,, Sanghwa,Ragunathan, Kaushik,Joo, Chirlmin,Ha, Taekjip,Hohng, Sungchul WILEY‐VCH Verlag 2010 Angewandte Chemie Vol.122 No.51

<P><B>Sechs FRET‐Interfluorophoreffizienzen</B> <I>E</I><SUB><I>ij</I></SUB> (siehe Schema) wurden in Echtzeit durch eine Einzelmolekül‐Vierfarben‐FRET‐Technik mittels konfokaler Mikroskopie sowie Fluoreszenzmikroskopie mit interner Totalreflexion bestimmt. Das Verfahren wurde verwendet, um die korrelierte Bewegung der vier Arme der Holliday‐Junction und die Korrelation von RecA‐vermittelten Strangaustauschereignissen an beiden Enden eines synaptischen Komplexes zu untersuchen.</P>

Effect of Geumgoeshingi-Whan Pharmacopuncture on the Blood Pressure in Spontaneously Hypertensive Rats

Lee, Meesun,Lee, Sangmi,Jeong, Hohyn,Park, Manyong,Kim, Dong-Woung,Song, Bong-Keun,Lee, Jong-Deok,Lee, Ho Sub,Kim, Sungchul KOREAN PHARMACOPUNCTURE INSTITUTE 2013 Journal of pharmacopuncture Vol.16 No.1

Objective: The aim of this experiment was to investigate the effect and the mechanism of Geumgoeshingi-whan (GGSGW) Pharmacopuncture at the acupoint GV 4 on the blood pressure in spontaneously hypertensive rats (SHR). Methods: SHR were injected with normal saline solution (Control-SHR group)or GGSGW Pharmacopuncture (GGSGW-SHR group) at the acupoint GV 4. The systolic arterial blood pressure and renal parameters were measured for two weeks. Results: The systolic arterial blood pressure was decreased significantly after GGSGW Pharmacopuncture at the acupoint GV 4 in SHR, followed by a significant rise in creatine clearance. The plasma levels of aldosterone were decreased significantly after GGSGW Pharmacopunctureas were the plasma levels of atrial natriuretic peptide (ANP). Conclusion: These results suggest that the blood pressure was decreased significantly after GGSGW Pharmacopuncture at the acupoint GV 4 in SHR and that the depressor response of the blood pressure was related to decreases in the plasma levels of aldosterone and ANP.

The Effects of Acupotomy Therapy on Carpal Tunnel Syndrome: A Report of 4 Cases

Lee, Seongjin,Cha, Eunhye,Yang, Muhak,Lee, Jongdeok,Lee, Jiyoung,Lee, Sojin,Kim, Deokho,Kim, Sungchul Korean AcupunctureMoxibustion Medicine Society 2018 Korean Journal of Acupuncture Vol.35 No.1

The aim of this report was to show the effects of acupotomy in patients with carpal tunnel syndrome. Four patients were treated with acupotomy twice. Visual analogue scale (VAS), Tinel's sign, Phalen's test, Boston carpal tunnel syndrome questionnaire (BCTQ), muscular strength test, and a cross-sectional area of median nerve was measured using ultrasound before and after treatment. In all 4 cases, the VAS score, BCTQ score and cross-sectional area of median nerve, all decreased and muscular strength test score increased. Tinel's sign and the Phalen's test changed from a positive to a negative in most cases. This report shows that acupotomy is an effective treatment for carpal tunnel syndrome. Further larger are needed to fully evaluate the beneficial effects of this treatment.

Analysis of Prey of Mandarin Fish and Large Mouth Bass and Distribution of Fish Population in Lake Paro, Korea

Jaeyong Lee,Kwang yeol Lee,Sungchul Park,Jaeseok Choi,Hong gi Jang,Joon Chul Kim 한국환경생태학회 2015 한국환경생태학회지 Vol.29 No.2

We quantified temporal and spatial changes in the habitat for fish populations, the distribution of mandarin fish(Siniperca scherzeri) and an introduced species, largemouth bass(Micropterus salmoides) in Lake Paro and inflowing streams. The number of fish species identified in Lake Paro and the tributary streams included 10 families, 24 species and 10 families 30 species, respectively. The dominant fish species in Lake Paro were Zacco platypus, Hemibarbus labeo, Squalidus gracilis majimae, S. scherzeri and Tridentiger brevispinis, Z. platypus, Z. koreanus, and S. gracilis majimae in the inflowing streams. Although the habitat segregation for S. scherzeri and M. salmoides occurs, these two species showed the use of the fishes of the family Gobiidae as an important prey item based on IRI analysis. S. scherzeri and M. salmoides preyed mainly on T. brevispinis(67.4 %) and R. brunneus(84.0 %), respectively. The species preyed on by S. scherzeri and M. salmoides were benthic fishes that inhabit shallow water depths around the lake and have little swimming ability.

압전 에너지 하베스팅을 위한 자체 리셋 스위칭 정류기 회로 설계

이준기(Junki Lee),이성철(SungChul Lee),이강윤(Kang-Yoon Lee),이성호(Sungho Lee) 대한전자공학회 2016 대한전자공학회 학술대회 Vol.2016 No.6

학습 지원 도구로서의 서술형 평가 그리고 인공지능의 활용: WA³I 프로젝트 사례

하민수 ( Minsu Ha ),이경건 ( Gyeong-geon Lee ),신세인 ( Sein Shin ),이준기 ( Jun-ki Lee ),최성철 ( Sungchul Choi ),주재걸 ( Jaegul Choo ),김남형 ( Namhyoung Kim ),이현주 ( Hyeonju Lee ),이종호 ( Jongho Lee ),이주림 ( Jurim Lee ),조 한국현장과학교육학회 2019 현장과학교육 Vol.13 No.3

본 연구는 인공지능을 활용한 서술형 평가 문항 자동채점 프로그램 WA³I을 개발하였으며, 이 프로그램이 학습 지원 도구로서 활용되기 위한 방안에 대하여 논하였다. 먼저 컴퓨터를 활용한 서술형 평가 자동채점에 대한 기존 연구들을 정리하고, 본 연구에서 개발한 서술형 평가 자동채점 프로그램 WA³I의 개발 과정을 소개하였다. 본 연구에서는 31개의 서술형 평가 문항을 개발하고 이에 대한 학생의 응답을 수집하였으며, 이 중 컴퓨터와 전문가 채점간의 일치도가 높은 19개의 문항에 대한 자동채점 모델을 개발하였다. 이 자동채점 모델로 채점 가능한 유사 문항 11개를 더 개발하여 총 30개의 문항을 웹상에서 WA³I ver.1으로 구현하였다. 효과적인 교수ㆍ학습을 위해 본 연구에서 개발된 서술형 자동채점 프로그램 WA³I ver.1의 개선 과제 및 추후 연구 방향에 대해 논하였다. 이러한 내용은 현장과학교사들의 평가 계획 및 실행에 영향을 줄 수 있을 것으로 기대된다. This study developed WA³I, which is automated computer scoring system in open-ended assessment using artificial intelligent and discussed how to utilize WA³I as a learning-support tool. To do this, the previous research dealing with automated computer scoring system has been reviewed and explained the development process of WA³I in Korea. We developed 31 open-ended questions and collected the students’ responses. Among the 31 questions, nineteen questions showing the high agreement between computer and human scoring were selected and scoring model were developed. In additions to selected nineteen questions, eleven questions were added as the prototype of web-based WA³I ver.1. We discussed how it can be improved in order to facilitate the effective teaching and learning. This paper is expected to give an implication to the science teachers’ assessment plan and practice.

Genetic specificity study using next-generation sequencing (NGS) of peritoneal metastatic colorectal cancer compared to primary colorectal cancer

Lee Sungchul,Shin Wonseok,Park Dong-Guk,Namgung Hwan 한국유전학회 2024 Genes & Genomics Vol.46 No.3

Background In patients with colorectal cancer, peritoneal metastases are the second most frequent metastatic lesion after liver metastases. Peritoneal metastases have a very poor prognosis, with a median survival time of 5–7 months. Currently, there is a lack of research on the genetic differences between primary colorectal cancer and peritoneal metastases. Therefore, we aimed to identify their genetic characteristics through a cancer panel test using next-generation sequencing. Objective We aim to investigate the specificity of genetic variants in primary colorectal cancer and peritoneal metastases. Methods We recruited patients with stage I, II, and III primary colorectal cancer and peritoneal metastases for genetic analysis using NGS. Samples were collected from patients who underwent surgery at Dankook University Hospital and consented to genetic testing. NGS was performed using a cancer panel. Results Among 36 patients with primary cancer, TP53 gene mutation was identified the most in 25 patients (69%), followed by APC gene mutation in 19 patients (53%), and KRAS gene mutation in 17 patients (47%). In the peritoneal metastasis patient group, unlike the primary cancer patient group, KRAS gene mutations were the most common 6 patients (55%), followed by TP53 gene mutations in 4 patients (36%) and PIK3CA gene mutations in 2 patients (18%). Conclusion The small number of surgical cases of peritoneal metastases was a limitation of our sample size. Nevertheless, we identified differences in the alterations of specific genes between primary and peritoneal metastases. Acquiring additional cases and collecting more data will provide deeper insights into these cancers. Background In patients with colorectal cancer, peritoneal metastases are the second most frequent metastatic lesion after liver metastases. Peritoneal metastases have a very poor prognosis, with a median survival time of 5–7 months. Currently, there is a lack of research on the genetic differences between primary colorectal cancer and peritoneal metastases. Therefore, we aimed to identify their genetic characteristics through a cancer panel test using next-generation sequencing. Objective We aim to investigate the specificity of genetic variants in primary colorectal cancer and peritoneal metastases. Methods We recruited patients with stage I, II, and III primary colorectal cancer and peritoneal metastases for genetic analysis using NGS. Samples were collected from patients who underwent surgery at Dankook University Hospital and consented to genetic testing. NGS was performed using a cancer panel. Results Among 36 patients with primary cancer, TP53 gene mutation was identified the most in 25 patients (69%), followed by APC gene mutation in 19 patients (53%), and KRAS gene mutation in 17 patients (47%). In the peritoneal metastasis patient group, unlike the primary cancer patient group, KRAS gene mutations were the most common 6 patients (55%), followed by TP53 gene mutations in 4 patients (36%) and PIK3CA gene mutations in 2 patients (18%). Conclusion The small number of surgical cases of peritoneal metastases was a limitation of our sample size. Nevertheless, we identified differences in the alterations of specific genes between primary and peritoneal metastases. Acquiring additional cases and collecting more data will provide deeper insights into these cancers.

KCHO-1, a Novel Antineuroinflammatory Agent, Inhibits Lipopolysaccharide-Induced Neuroinflammatory Responses through Nrf2-Mediated Heme Oxygenase-1 Expression in Mouse BV2 Microglia Cells

Lee, Dong-Sung,Ko, Wonmin,Yoon, Chi-Su,Kim, Dong-Cheol,Yun, Jinju,Lee, Jun-Kyung,Jun, Ki-Young,Son, Ilhong,Kim, Dong-Woung,Song, Bong-Keun,Choi, Seulah,Jang, Jun-Hyeog,Oh, Hyuncheol,Kim, Sungchul,Kim, Hindawi Publishing Corporation 2014 Evidence-based Complementary and Alternative Medic Vol.2014 No.-

<P>The brain is vulnerable to oxidative stress and inflammation that can occur as a result of aging or neurodegenerative diseases. Our work has sought to identify natural products that regulate heme oxygenase (HO)-1 and to determine their mechanism of action in neurodegenerative diseases. KCHO-1 is a novel herbal therapeutic containing 30% ethanol (EtOH) extracts from nine plants. In this study, we investigated the antineuroinflammatory effects of KCHO-1 in lipopolysaccharide- (LPS-) treated mouse BV2 microglia. KCHO-1 inhibited the protein expression of inducible nitric oxide synthase (iNOS), iNOS-derived nitric oxide (NO), cyclooxygenase- (COX-) 2, and COX-2-derived prostaglandin E2 (PGE<SUB>2</SUB>) in LPS-stimulated BV2 microglia. It also reduced tumor necrosis factor-<I>α</I> (TNF-<I>α</I>), interleukin-1<I>β</I> (IL-1<I>β</I>), and IL-6 production. This effect was correlated with the suppression of inhibitor of nuclear factor kappa B-<I>α</I> (I<I>κ</I>B-<I>α</I>) phosphorylation and degradation and nuclear factor kappa B (NF-<I>κ</I>B) translocation and DNA binding. Additionally, KCHO-1 upregulated HO-1 expression by promoting nuclear translocation of nuclear factor E2-related factor 2 (Nrf2) in mouse BV2 microglia. Tin protoporphyrin (SnPP), an HO activity inhibitor, was used to verify the inhibitory effects of KCHO-1 on proinflammatory mediators and proteins associated with HO-1 expression. Our data suggest that KCHO-1 has therapeutic potential in neurodegenerative diseases caused by neuroinflammation.</P>

Analysis of Prey of Mandarin Fish and Large Mouth Bass and Distribution of Fish Population in Lake Paro, Korea

Lee, Jaeyong,Lee, Kwang Yeol,Park, Sungchul,Choi, Jaeseok,Jang, Hong Gi,Kim, Joon Chul Korean Society of Environment and Ecology 2015 한국환경생태학회지 Vol.29 No.2

We quantified temporal and spatial changes in the habitat for fish populations, the distribution of mandarin fish(Siniperca scherzeri) and an introduced species, largemouth bass(Micropterus salmoides) in Lake Paro and inflowing streams. The number of fish species identified in Lake Paro and the tributary streams included 10 families, 24 species and 10 families 30 species, respectively. The dominant fish species in Lake Paro were Zacco platypus, Hemibarbus labeo, Squalidus gracilis majimae, S. scherzeri and Tridentiger brevispinis, Z. platypus, Z. koreanus, and S. gracilis majimae in the inflowing streams. Although the habitat segregation for S. scherzeri and M. salmoides occurs, these two species showed the use of the fishes of the family Gobiidae as an important prey item based on IRI analysis. S. scherzeri and M. salmoides preyed mainly on T. brevispinis(67.4 %) and R. brunneus(84.0 %), respectively. The species preyed on by S. scherzeri and M. salmoides were benthic fishes that inhabit shallow water depths around the lake and have little swimming ability.

Global burden of primary liver cancer and its association with underlying aetiologies, sociodemographic status, and sex differences from 1990–2019: A DALY-based analysis of the Global Burden of Disease 2019 study

Sungchul Choi,Beom Kyung Kim,Dong Keon Yon,Seung Won Lee,Han Gyeol Lee,Ho Hyeok Chang,Seoyeon Park,Ai Koyanagi,Louis Jacob,Elena Dragioti,Joaquim Radua,Jae Il Shin,Seung Up Kim,Lee Smith 대한간학회 2023 Clinical and Molecular Hepatology(대한간학회지) Vol.29 No.2

Background/Aims: Global distribution of dominant liver cancer aetiologies has significantly changed over the past decades. This study analyzed the updated temporal trends of liver cancer aetiologies and sociodemographic status in 204 countries and territories from 1990 to 2019. Methods: The Global Burden of Disease 2019 report was used for statistical analysis. In addition, we performed stratification analysis to five quintiles using sociodemographic index and 21 geographic regions. Results: The crude numbers of liver cancer disease-adjusted life years (DALYs) and deaths significantly increased during the study period (DALYs; 11,278,630 in 1990 and 12,528,422 in 2019, deaths; 365,215 in 1990 and 484,577 in 2019). However, the Age-standardized DALY and mortality rates decreased. Hepatitis B virus (HBV) remains the leading cause of liver cancer DALYs and mortality, followed by hepatitis C virus (HCV), alcohol consumption, and non-alcoholic steatohepatitis/non-alcoholic fatty liver disease (NASH/NAFLD). Although Age-standardized DALY and mortality rates of liver cancer due to HBV and HCV have decreased, the rates due to alcohol consumption and NASH/NAFLD have increased. In 2019, the population of the East Asia region had the highest Age-standardized DALY and mortality rates, followed by high-income Asia-Pacific and Central Asia populations. Although East Asia and high-income Asia-Pacific regions showed a decrease during the study period, Age-standardized DALY rates increased in Central Asia. High-income North American and Australasian populations also showed a significant increase in Age-standardized DALY. Conclusions: Liver cancer remains an ongoing global threat. The burden of liver cancer associated with alcohol consumption and NASH/NAFLD is markedly increasing and projected to continuously increase.