Parthenolide-Induced Apoptosis, Autophagy and Suppression of Proliferation in HepG2 Cells

Sun, Jing,Zhang, Chan,Bao, Yong-Li,Wu, Yin,Chen, Zhong-Liang,Yu, Chun-Lei,Huang, Yan-Xin,Sun, Ying,Zheng, Li-Hua,Wang, Xue,Li, Yu-Xin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.12

Purpose: To investigate the anticancer effects and underlying mechanisms of parthenolide on HepG2 human hepatocellular carcinoma cells. Materials and Methods: Cell viability was assessed by MTT assay and cell apoptosis through DAPI, TUNEL staining and Western blotting. Monodansylcadaverin(MDC) and AO staining were used to detect cell autophagy. Cell proliferation was assessed by Ki67 immunofluorescence staining. Results: Parthenolide induced growth inhibition in HepG2 cells. DAPI and TUNEL staining showed that parthenolide could increase the number of apoptotic nuclei, while reducing the expression of the anti-apoptotic protein Bcl-2 and elevating the expression of related proteins, like p53, Bax, cleaved caspase9 and cleaved caspase3. Parthenolide could induce autophagy in HepG2 cells and inhibited the expression of proliferation-related gene, Ki-67. Conclusions: Parthenolide can exert anti-cancer effects by inducing cell apoptosis, activating autophagy and inhibiting cell proliferation.

Cloning and differential expression of three heat shock protein genes associated with thermal stress from the wolf spider Pardosa pseudoannulata (Araneae: Lycosidae)

Sun Liang-Yu,Liu Jing,Li Qin,Fu Di,Zhu Jia-Yun,Guo Jian-Jun,Xiao Rong,Jin Dao-Chao 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.1

Pardosa pseudoannulata is the main predatory natural enemy of crop pests in a paddy ecosystem. When P. pseudoannulata is exposed to unfavorable temperature conditions, the response of heat shock proteins could resist the damage, and is therefore, conducive to the organism’s rapid adaptation to the surrounding stress environ ment. In this study, we explored the roles of hsp70 and hsp90 genes in response to heat stress, using the rapid amplification of cDNA ends technique and cloned full-length cDNAs of Pphsp70, Pphsp83, and Pphsp90. The mRNA expression levels of the three genes under different temperature stresses (25, 28, 31, 34, 37, 40, and 43 ◦ C) and with different duration stresses (4, 8, 12, 16, and 20 h) were analyzed by quantitative real-time polymerase chain reaction. The full-length cDNA of Pphsp70, Pphsp83, and Pphsp90 was 2331 base pair (bp), 2466 bp, and 2663 bp, respectively. Phylogenetic analysis of amino acid sequences of Pphsp70, Pphsp83, and Pphsp90 showed that the sequences had high homology with that of other spiders. The mRNA expression of all three genes was extremely significantly up-regulated at 43 ◦ C. Moreover at 43 ◦ C, the expression of all three genes in both female and male spiders at the duration of 4 h was the highest compared to that of other stress duration groups. Therefore, it can be inferred that the three genes of P. pseudoannulata play a crucial protective role in resistance in a high-temperature environment.

Primary survey of avian influenza virus and Newcastle disease virus infection in wild birds in some areas of Heilongjiang Province,China

Yu-Ping Hua,Hong-Liang Chai,Si-Yuan Yang,Xiang-Wei Zeng,Ying Sun 대한수의학회 2005 Journal of Veterinary Science Vol.6 No.4

Two hundred thirty specimens of wild birds were collected from some areas in Heilongjiang Province during the period of 2003~2004, including two batches of specimens collected randomly from a same flock of mallards in Zhalong Natural Reserve in August and December, 2004, respectively. Primary virus isolation and identification for avian influenza virus (AIV) and Newcastle disease virus (NDV) were performed. The results showed that only two specimens of young mallards collected from Zhalong Natural Reserve in August, 2004 were positive to AIV (isolation rate 0.9%), and one strain (D57) of these two virus isolates was identified to be H9 subtype by hemagglutination inhibition test. Meanwhile, the two batches of blood serum samples of mallards from Zhalong were also examined for antibodies against AIV and NDV. Among 38 blood serum samples collected in August, antibodies against the hemagglutinin of H1, H3, H5, H6 and H9 subtypes of AIV were found in 1, 0, 2, 0 and 8 samples, respectively; and 11 samples were found with antibody against NDV. Whereas the NDV isolation in both two batches of specimens of mallard was negative, all of the 32 blood serum samples collected in December were negative for antibodies against AIV and NDV.

The Classic Security Application in M2M: the Authentication Scheme of Mobile Payment

( Liang Hu ),( Ling Chi ),( Hong-tu Li ),( Wei Yuan ),( Yu Yu Sun ),( Jian-feng Chu ) 한국인터넷정보학회 2012 KSII Transactions on Internet and Information Syst Vol.6 No.1

As one of the four basic technologies of IOT (Internet of Things), M2M technology whose advance could influence on the technology of Internet of Things has a rapid development. Mobile Payment is one of the most widespread applications in M2M. Due to applying wireless network in Mobile Payment, the security issues based on wireless network have to be solved. The technologies applied in solutions generally include two sorts, encryption mechanism and authentication mechanism, the focus in this paper is the authentication mechanism of Mobile Payment. In this paper, we consider that there are four vital things in the authentication mechanism of Mobile Payment: two-way authentication, re-authentication, roaming authentication and inside authentication. Two-way authentication is to make the mobile device and the center system trust each other, and two-way authentication is the foundation of the other three. Re-authentication is to re-establish the active communication after the mobile subscriber changes his point of attachment to the network. Inside authentication is to prevent the attacker from obtaining the privacy via attacking the mobile device if the attacker captures the mobile device. Roaming authentication is to prove the mobile subscriber`s legitimate identity to the foreign agency when he roams into a foreign place, and roaming authentication can be regarded as the integration of the above three. After making a simulation of our proposed authentication mechanism and analyzing the existed schemes, we summarize that the authentication mechanism based on the mentioned above in this paper and the encryption mechanism establish the integrate security framework of Mobile Payment together. This makes the parties of Mobile Payment apply the services which Mobile Payment provides credibly.

High Temperature Oxidation Resistance of NiCoCrAl High Entropy Alloy Coating on the TiAl Alloy

Yuanyuan Sun,Qiang Miao,Wenping Liang,Kai Zang,Yuting Wu,Haiyang Yu,Mengjuan Yin,Xiguang Gao,Yindong Song 대한금속·재료학회 2024 METALS AND MATERIALS International Vol.30 No.1

The high entropy alloy coating is considered as one of the most promising methods to improve the high-temperature oxidationresistance of titanium aluminum alloys due to its fine mechanical property and thermal stability. However, the high entropyalloy coating prepared so far has poor coating quality, and low coating forming efficiency and there are certain requirementsfor the substrate alloys. To this end, a NiCoCrAl high entropy alloy coating was developed using the double glow plasmaalloying technique on the TiAl alloy. The morphology, phase structure, and high temperature oxidation resistance of thecoating were comprehensively studied. The results indicate that a uniform and dense coating with a single solid solution offace-centered cube phase formed on the alloy surface. Furthermore, isothermal oxidation test was performed, and the oxidationfailure process of the TiAl substrate and the NiCoCrAl-coated sample with different oxidation times was analyzed. It isfound that the coating efficiently prevented the TiAl alloy from degeneration for up to 100 h at 900 °C.

Antiviral Activities of Natural Compounds Derived from Traditional Chinese Medicines against Porcine Circovirus Type 2 (PCV2)

Na Sun,Tong Yu,Jun-Xing Zhao,Yaogui Sun,Junbing Jiang,Zhi-Bian Duan,WenkuiWang,Yuan-Liang Hu,Hai-Min Lei,HongquanLi 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.1

Twenty natural compounds isolated fromtraditional Chinese medicines were examined for theirantiviral activities against PCV2 in vitro. Antiviral activitywas analyzed by the assays of blocking of virus entry,inhibition of virus replication and inactivation of virusparticles in the virus suspension. 50% cytotoxic concentration(CC50) and 50% effective concentration (EC50) for eachcompound were determined by using the MTT method andimmunofluorescence assay (IFA), respectively. The resultsobtained from the inhibition of virus replication andinactivation of virus particles showed that the maximuminhibition rates for matrine and scutellarin were 57 and72.69% and their SI were 29.87 and 18.66, respectively. However, the results from the assays of inhibition of virusentry and inhibition of virus replication demonstrated thatboth aesculetin and arecoline hydrochloride stimulatedPCV2 replication, with the maximum stimulation rate of387.3 and 219.4%. In summary, both matrine and scutellarinhad antiviral activity against PCV2, while aesculetin andarecoline hydrochloride promoted PCV2 infection replicationin vitro.

Control System Design of Anti-rolling Tank Swing Bench Using BP Neural Network PID Based on LabVIEW

Lihua Liang,Mingxiao Sun,Songtao Zhang,Yu Wen,Peng Zhao,Jia Yuan 보안공학연구지원센터 2015 International Journal of Smart Home Vol.9 No.6

The anti-rolling tank swing bench is a typical hydraulic position servo system. Its function is to simulate the ship roll motion and to verify the performance of the anti-rolling tank. Generally, the PID control method can achieve an ideal control effect. But the uncertainties, such as the couplings and the nonlinearty of the swing bench, make it difficult to establish the precise mathematical model of system. During experiments, the different disturbance torques are generated according to the different dimension scales of the anti-rolling tanks. Changes of the natural characteristics of the system are caused by changes of load, and the fixed parameters of PID controller are difficult to continue to ensure a good control effect. In order to reduce the influences caused by the factors mentioned above, a BP neural network PID control system based on virtual instrument LabVIEW is designed. By simulating the responses of the system in real time control, the verification tests using actual system are avoided. Finally, the experiments of swing bench are carried out. The results show that the controller can ensure the control accuracy and stability. The parameters tuning process efficiency is improved. The operation is convenient. The data acquisition process is simple and practical.

Association of Six Susceptibility Loci with Prostate Cancer in Northern Chinese Men

Zhang, Yu-Rong,Xu, Yong,Yang, Kuo,Liu, Ming,Wei, Dong,Zhang, Yao-Guang,Shi, Xiao-Hong,Wang, Jian-Ye,Yang, Fan,Wang, Xin,Liang, Si-Ying,Zhao, Cheng-Xiao,Wang, Fei,Chen, Xin,Sun, Liang,Zhu, Xiao-Quan,Zh Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12

Background/Aim: Six prostate cancer (PCa) susceptibility loci were identified in a genome-wide association study (GWAS) in populations of European decent. However, the associations of these 6 single-nucleotide polymorphisms (SNPs) with PCa has remained tobe clarified in men in Northern China. This study aimed to explore the loci associated with PCa risk in a Northern Chinese population. Methods: Blood samples and clinical information of 289 PCa patients and 288 controls from Beijing and Tianjin were collected. All risk SNPs were genotyped using polymerase chain reaction (PCR)-high resolution melting curve technology and gene sequencing. Associations between PCa and clinical covariates (age at diagnosis, prostate-specific antigen [PSA], Gleason score, tumor stage, and level of aggressiveness) and frequencies of alleles and genotypes of these SNPs were analyzed using genetic statistics. Results: Among the candidate SNPs, 11p15 (rs7127900, A) was associated with PCa risk (P = 0.02, odds ratio [OR] = 1.64, 95% confidence interval [CI] = 1.09-2.46). Genotypes showed differences between cases and controls on 11p15 (rs7127900, A), 11q13 (rs7931342, T), and HNF1B (rs4430796, A) (P = 0.03, P = 0.01, and P = 0.04, respectively). The genotype TG on 11q13 (rs7931342, T) was positively associated with an increased Gleason score (P = 0.04, OR = 2.15, 95% CI = 1.02-4.55). Patients carrying TG on 17q24 (rs1859962, G) were negatively associated with an increased body mass index (BMI) (P = 0.03, OR = 0.44, 95% CI = 0.21-0.92) while those with AG on HNF1B (rs4430796, A) were more likely to have PSA increase (P = 0.002). Conclusion: Our study suggests that 11p15 (rs7127900, A) could be a susceptibility locus associated with PCa in Northern Chinese. Genotype TG on 11q13 (rs7931342, T) could be related to an increased Gleason score, AG on HNF1B (rs4430796, A) could be associated with PSA increase, and TG on 17q24 (rs1859962, G) could be negatively associated with an increased BMI in Chinese men with PCa.

Determination of Boanmycin in Pharmaceutical Preparations by a Simple and Rapid Ion-pair LC Method

Liu, Yu,Yoo, Sun Dong,Fang, Liang,Jin, Xin,Li,Ren, YanJun,Yao, ChongShun,Li, TieFu Springer-Verlag 2009 Chromatographia Vol.70 No.3

Error Estimation Method for Matrix Correlation-Based Wi-Fi Indoor Localization

( Yong-liang Sun ),( Yu-bin Xu ) 한국인터넷정보학회 2013 KSII Transactions on Internet and Information Syst Vol.7 No.11

A novel neighbor selection-based fingerprinting algorithm using matrix correlation (MC) for Wi-Fi localization is presented in this paper. Compared with classic fingerprinting algorithms that usually employ a single received signal strength (RSS) sample, the presented algorithm uses multiple on-line RSS samples in the form of a matrix and measures correlations between the on-line RSS matrix and RSS matrices in the radio-map. The algorithm makes efficient use of on-line RSS information and considers RSS variations of reference points (RPs) for localization, so it offers more accurate localization results than classic neighbor selection-based algorithms. Based on the MC algorithm, an error estimation method using artificial neural network is also presented to fuse available information that includes RSS samples and localization results computed by the MC algorithm and model the nonlinear relationship between the available information and localization errors. In the on-line phase, localization errors are estimated and then used to correct the localization results to reduce negative influences caused by a static radio-map and RP distribution. Experimental results demonstrate that the MC algorithm outperforms the other neighbor selection-based algorithms and the error estimation method can reduce the mean of localization errors by nearly half.