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Genome Wide Computational Identification of Tuna (Thunnus orientalis) MicroRNAs and Their Targets
Sangita Chowdhury Paul,Ashutosh Sharma,Richa Mehta,Sujay Paul 한국해양과학기술원 2018 Ocean science journal Vol.53 No.4
Applying genome-wide computational-based approaches (using the draft genome sequence published in recent years) and following a set of strict filtering criteria, a total of 48 potentially conserved microRNAs belonging to 30 families were identified from economically important fish tuna (Thunnus orientalis). Using BLAST and RNA hybrid program a total of 19 potential miRNA targets were also identified in this study, which includes a number of signaling molecules (serine/threonine-protein kinase, GTPase activating protein etc.) and transcription factors (F-box protein, zinc finger protein etc.). This study provides the basis for gaining a better understanding of miRNA-mediated gene regulatory processes in fishes.