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Kenji Suetsugu,Naoyuki Nakahama 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.3
To protect endangered and commercially important orchid species, it is important to monitor and accurately identify orchid pests. Japanagromyza tokunagai (Sasakawa) larvae are known to infest the flower buds, stems, and ovaries of various Japanese orchid species. Given that J. tokunagai can infest diverse orchid hosts, J. tokunagai has the potential to be an economically important orchid pest in greenhouses. However, damage by J. tokunagai has previously only been reported in outdoor habitats. Here we revealed that the dipteran larvae infesting the flower buds of Phalaenopsis aphrodite Rchb.f. (Orchidaceae) cultivated in a greenhouse are J. tokunagai based on DNA barcoding, using COI sequences. Infested flower buds remained closed and eventually dropped to the ground, making the P. aphrodite plants commercially worthless. Phalaenopsis aphrodite is one of the most economically important ornamental orchids. Therefore, J. tokunagai is a potential threat to orchid production and we recommend taking precautions to prevent its spread.
Novel Architecture of EER Transmitter with Class-E Amplifier
Tadashi Suetsugu,Xiuqin Wei,Shotaro Kuga 전력전자학회 2015 ICPE(ISPE)논문집 Vol.2015 No.6
This paper presents a novel architecture of the envelope elimination and restoration (EER) transmitter with the class-E amplifier. A design example is also given along with the PSpice-simuIation results. In the proposed architecture, a MOSFET is added and connected to the dc-feed inductance of the class-E amplifier in parallel, basing on the conventional Envelope Pulse Width Modulation (EPWM)-EER architecture. Therefore, it is possible to obtain no transient-attenuation performance, low surge voltage, and fast rising-time response by applying the proposed architecture. In other words, the proposed architecture can realize all performance of the improved EPWM-EER architecture, i.e., the Psuedo-EPWM or Preceding EPWM (pEPWM), but without carrying out the process of trial and error, which is required in the pEPWM architecture. The PSpice-simulation results show the effectiveness and validity of the proposed architecture.
Active Voltage Clamping of Class E Amplifier
Tadashi Suetsugu 전력전자학회 2011 ICPE(ISPE)논문집 Vol.2011 No.5
In this paper, a lossless voltage clamp method with an auxiliary switch is proposed as a remedy for the high peak switch voltage of the class E amplifier. In the proposed method, the auxiliary switch is operated with synchronized drive circuit. Output power can be regulated by adjusting phase of auxiliary switch.
Seed dispersal by ants in the fully mycoheterotrophic plant Sciaphila secundiflora (Triuridaceae)
Kenji Suetsugu,Takuto Shitara,Akira Yamawo 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.3
The tiny seeds, or dust seeds, of heterotrophic plants are usually dispersed by wind. However, most mycoheterotrophic plants grow in the understory of densely vegetated forests, where the wind is less reliable and wind dispersal is likely less efficient. In the present study, we found that seeds of the fully mycoheterotrophic plant Sciaphila secundiflora (Triuridaceae) possess elaiosomes, and that at least one species of ant, Nylanderia flavipes, functions as a dispersal agent of the plant's seeds. This is the first demonstration of myrmecochory in mycoheterotrophic plants, as well as the first report of zoochory in Triuridaceae.
Kenji Suetsugu,Toshiharu Mita 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.4
Agromyzid flies, also known as leafminer flies, are one of the most serious insect pests that infest various plants. An agromyzid fly Japanagromyza tokunagai lays eggs in the ovaries of various Japanese orchids during or shortly after anthesis. Consequently, seed predation by J. tokunagai has the potential to severely limit the reproduction of many endangered orchids in Japan. While agromyzid populations tend to be suppressed by rich communities of natural parasitoids, such parasitoids have yet to be reported. Here we report Pediobius metallicus as the first record of a parasitoid of J. tokunagai. It is highly unusual to find parasitoids infesting J. tokunagai, possibly because the fly is protected by the thick wall and three-dimensional structure of orchid capsule. Pediobius metallicus may play an important role in suppressing J. tokunagai population at least in our study site.
Systematic time-dependent visualization and quantitation of the neurogenic rate in brain organoids
Kosodo, Yoichi,Suetsugu, Taeko,Kobayashi, Tetsuya J.,Matsuzaki, Fumio Elsevier 2017 Biochemical and biophysical research communication Vol. No.
<P><B>Abstract</B></P> <P>Organoids mimicking the formation of the brain cortex have been demonstrated to be powerful tools for developmental studies as well as pathological investigations of brain malformations. Here, we report an integrated approach for the quantification of temporal neural production (neurogenic rate) in organoids derived from embryonic brains. Spherical tissue fragments with polarized cytoarchitectures were incubated in multiple cavities arranged in a polymethylmethacrylate chip. The time-dependent neurogenic rate in the organoids was monitored by the level of EGFP under the promoter of <I>Tbr2</I>, a transcription factor that is transiently expressed in neural fate-committed progenitors during corticogenesis. Importantly, our monitoring system exhibited a quick response to DAPT, a drug that promotes neural differentiation. Furthermore, we successfully quantified the temporal neurogenic rate in a large number of organoids by applying image processing that semi-automatically recognized the positions of organoids and measured their signal intensities from sequential images. Taken together, we provide a strategy to quantitate the neurogenic rate in brain organoids in a time-dependent manner, which will also be a potent method for monitoring organoid formation and drug activity in other tissue types.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We established a semi-automatic neurogenic activity assay system in brain organoids. </LI> <LI> Microcavity chips and image processing enabled the accurate scoring of fluorescence. </LI> <LI> The temporal response to a drug that potentiates neurogenesis was demonstrated. </LI> </UL> </P>
Takeshi Yasukouchi,Tadashi Suetsugu 대한전자공학회 2008 ITC-CSCC :International Technical Conference on Ci Vol.2008 No.7
This paper analyzes maximum output power of class E amplifier with arbitrary transistor. It is important to estimate maximum output power of class E amplifier when a specification of transistor is given. In this paper, values of circuit parameters that gives maximum output power for given operating frequency, dc supply voltage, and output capacitance of the transistor are calculated. This paper presents scheme to calculate circuit parameters that maximum output power in this condition. Finally, some example calculations are shown when this scheme was applied to some actual power MOSFET transistors.
Numerical simulation of the crack propagation behavior in 3D elastic body
Taniguchi, Takeo,Miyaji, Akihiko,Suetsugu, Takeshi,Matsunaga, Shohgo Techno-Press 1994 Structural Engineering and Mechanics, An Int'l Jou Vol.2 No.3
The purpose of this investigation is to propose a numerical simulation method of the crack propagation behavior in 3-dimensionl elastic body. The simulation method is based on the displacement-type finite element method, and the linear fracture theory is introduced. The results from the proposed method are compared with those from the structural experiments, and the good coincidences between them are shown in this paper. At the same time, 2-dimensional analysis is also done, and the results are compared with those obtained from 3-dimensional analysis and the structural experiments.