IL-4 increases type 2, but not type 1, cytokine production in CD8 ⁺ T cells from mild atopic asthmatics

Stanciu, Luminita A,Roberts, Kevan,Papadopoulos, Nikolaos G,Cho, Sang-Heon,Holgate, Stephen T,Coyle, Anthony J,Johnston, Sebastian L BioMed Central 2005 Respiratory research Vol.6 No.-

<P><B>Background</B></P><P>Virus infections are the major cause of asthma exacerbations. CD8<SUP>+ </SUP>T cells have an important role in antiviral immune responses and animal studies suggest a role for CD8<SUP>+ </SUP>T cells in the pathogenesis of virus-induced asthma exacerbations. We have previously shown that the presence of IL-4 during stimulation increases the frequency of IL-5-positive cells and CD30 surface staining in CD8<SUP>+ </SUP>T cells from healthy, normal subjects. In this study, we investigated whether excess IL-4 during repeated TCR/CD3 stimulation of CD8<SUP>+ </SUP>T cells from atopic asthmatic subjects alters the balance of type 1/type 2 cytokine production in favour of the latter.</P><P><B>Methods</B></P><P>Peripheral blood CD8<SUP>+ </SUP>T cells from mild atopic asthmatic subjects were stimulated <I>in vitro </I>with anti-CD3 and IL-2 ± excess IL-4 and the expression of activation and adhesion molecules and type 1 and type 2 cytokine production were assessed.</P><P><B>Results</B></P><P>Surface expression of very late antigen-4 [VLA-4] and LFA-1 was decreased and the production of the type 2 cytokines IL-5 and IL-13 was augmented by the presence of IL-4 during stimulation of CD8<SUP>+ </SUP>T cells from mild atopic asthmatics.</P><P><B>Conclusion</B></P><P>These data suggest that during a respiratory virus infection activated CD8<SUP>+ </SUP>T cells from asthmatic subjects may produce excess type 2 cytokines and may contribute to asthma exacerbation by augmenting allergic inflammation.</P>

Hometown: The Topography of Initiation and Catharsis in Yun Hǔnggil’s “Road to Soradan”

( Catalina Stanciu ) 서울대학교 규장각한국학연구원 2015 Seoul journal of Korean studies Vol.28 No.2

This paper deals with one of Yun Hǔnggil’s (b. 1942) latest short stories, “Road to Soradan” (Soradan kanǔn kil) (2002), and it has two purposes. The first is to depict the child narrator’s process of initiation during the Korean War (1950-1953) in relation to his hometown topography. More specifically, I will argue that being initiated into the surrounding world and the war circumstances is reflected through the child narrator’s direct interaction with Soradan, a pine tree groove in his hometown. At Soradan, the encounter between the child narrator (Yi Kigon) and Pak Chǔngsǒ has both a psychological and formative impact on the child narrator psyche. The second aim of this study is to interpret the adult narrator’s homecoming journey as endowed with cathartic connotations. Together with his former schoolmates, the narrator travels back both in space and in time, attempting to stitch back together the shattered memories of his traumatic childhood. Arguing the significance of Soradan in relation to the story’s narrator, I will suggest that it has multiple symbolic functions. On the one hand, how it affects the child narrator’s inner world and contributes to his understanding of life, circumstances of national division, and himself as an integrated part of this particular landscape; and on the other hand, the role of Soradan as the testimony for the Korean War’s legacy.

The Effects of War and the Strategies for Salvation in Postwar Korean Society in Jang Yonghak's The Poetry of John

Catalina Stanciu 한국학중앙연구원 한국학중앙연구원 2014 THE REVIEW OF KOREAN STUDIES Vol.17 No.2

This article discusses the effects of war and the strategies for salvation in postwar Korean circumstances as reflected in Jang Yonghak 張龍鶴 (1921-1999)’s story entitled The Poetry of John (요한詩集 1955). First of all, I attempt to argue that the effects of war are double-faceted: on one hand, there are the psychologically traumatic effects of war, and on the other hand there are the existential effects which accompany them. Therefore, war and postwar circumstances do not only imply victims’ psychological trauma, but also a state of existential angst in which victims question the value of human existence and doubt the chance for salvation. However, in those circumstances, the possibility for salvation might come with the finding of a “proper space.” The “proper space” shall be here bestowed with a metaphorical connotation of a location within which one can finally make full sense of oneself and of the world. Death is also been conferred a symbolic meaning of a possible space of salvation.

Places of Initiation in Yun Heunggil’s House of Twilight

Catalina Stanciu 한국학중앙연구원 한국학중앙연구원 2016 THE REVIEW OF KOREAN STUDIES Vol.19 No.2

This article deals with one of Yun Heunggil’s (b. 1942) novels, The House of Twilight (1970). The purpose of this article is to depict the child narrator’s process of initiation during the Korean War (1950-1953) in relation with two particular places in Jeongeup, a city in North Jeolla Province. Accompanied by a child named Gyeongju, the child narrator pays visits to an iron foundry and Gyeongju’s tavern house. Against the backdrop of these two spatial settings, the narrator interacts for the first time with the traumatic social impact of the War. He is introduced to the reality of death by Gyeongju’s recollection of her family’s trauma and through initiation into her sadistic games. Further analyzed in the article, these places, along with their ambivalent connotations, anticipate the narrator’s maturation.

Properties for an integral operator of -valent functions

N. Ularu,Laura Stanciu 장전수학회 2014 Advanced Studies in Contemporary Mathematics Vol.24 No.2

In this paper, we introduce two integral operators. The main object of the present paper is to obtain some conditions for these operators in the open unit disk.

Increased Interleukin-4, Interleukin-5, and Interferon-γ in Airway CD4⁺and CD8⁺T Cells in Atopic Asthma

Cho, Sang-Heon,Stanciu, Luminita A.,Holgate, Stephen T.,Johnston, Sebastian L. American Thoracic Society 2005 American journal of respiratory and critical care Vol.171 No.3

<P>Increased Th2 cytokine production in asthma is widely accepted, but excess production by asthmatic human airway CD4(+) T cells has not been demonstrated, nor has a relationship with disease severity. The importance of airway CD8(+) T cell type 1 and type 2 cytokine production in asthma is unknown. We investigated frequencies of IFN-gamma, interleukin (IL)-4 and IL-5 producing CD4(+) and CD8(+) blood and sputum T cells from normal subjects and subjects with asthma and compared between cell subsets, subject groups, and body compartments with and without in vitro stimulation and investigated relationships between cytokine production and asthma severity. Production of IL-4, IL-5, and IFN-gamma by unstimulated sputum CD4(+) and CD8(+) T cells was increased in subjects with asthma and related to disease severity, more for CD8(+) than for CD4(+) T cells. Frequencies of sputum CD8(+) T cells producing type 1 and type 2 cytokines were similar to those of CD4(+) T cells. In vitro stimulation polarized peripheral blood cytokine production toward IFN-gamma production, significantly more in subjects with asthma than in normal subjects. These data demonstrate increased type 1 and 2 cytokine production in CD4(+) and CD8(+) T cells in sputum and relate production to disease severity. Findings in blood did not reflect those in airways.</P>

Inactivation Kinetics of Alkaline Phosphatase from Different Species of Milk Using Quinolyl Phosphate as a Substrate

Loredana Dumitraşcu,Nicoleta Stănciuc,Silvius Stanciu,Gabriela Râpeanu 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.6

Kinetic studies of alkaline phosphatase (ALP)thermal inactivation in different types of milk were conductedusing a new fluorimetric technique. Inactivation experimentswere performed in a temperature range of 60-72.5oC. Abiphasic model provided a good description of milk ALPthermal inactivation. The kinetic parameters k and Ea weredetermined for heat labile and heat resistant isoenzymefractions. The inactivation rate of the heat labile fractionwas faster in skim milk than in whole milk. The extent ofthermal inactivation was different and showed that ALP isless thermoresistant in bovine milk.