Effects of monosodium glutamate and sucrose on the development of Drosophila melanogaster

Chung, Yong Jai,Kang, Soon Ja 梨花女子大學校 韓國生活科學硏究院 1974 韓國生活科學硏究院 論叢 Vol.12 No.-

초파리 (Drosophila melanogaster)의 발생에 대한 monosodium glutamate (MSG)와 sucrose의 영향을 보기 위하여 6가지 농도(0.0, 0.3, 7.0, 1.0, 15.0, 20.0%)의 MSG와 sucrose media에 두 계통의 초파리, 즉 Oregon-R와 Sinchon-I를 사육해서 우화에 나온 초파리의 수를 조사 검토한 결과는 다음 같다. 1) MSG의 처리는 초파리의 두 계통에서 다같이 그 농도가 증가함에 따라 발생에 억제적 효과를 나타내고 있으며 심지어 10%이상의 농도에서는 우화에 나오는 성충을 볼 수 없었다. 2) sucrose의 처리는 초파리 발생에 뚜렷한 억제적 효과를 나타내지는 않았으며, 동시에 촉진적 효과도 볼수 없었다. 3) Muller-5 test는 부정적 결과를 보였는데 이것은 MSG가 mutagenic effect가 없음을 말하는 것이다. 이상의 연구 결과로 미루어 보아, 전번 실험 (Chung4 Hong 1973) 결과와 마찬가지로 MSG는 분명히 초파리의 발생에 대하여 억제적 효과를 갖는 다고 볼 수 있다. In order to reexamine the results of the previous work (chung & Hong 1973) by using sucrose as a second control instead of table sugar which was used in the previous investigation and then to determine the ill effect of MSG on the development of Drosophila melanogaster, the present investigation was undertaken. The two strains of D. melanogaster, Oregon-R, Sinchon-I were used and MSG as well as sucrose (as the second control) media were prepared by adding MSG or sucrose at various concentrations to the standard food media for the present study. Ten flies (male 5, female 5) were placed in each vial and the numbers of F_1 adults emerging from it were counted. The results are presented below: 1) The numbers of F_1 flies decrease as the concentrations of MSG increase, no flies emerge from the medium 10.0% or greater concentration of MSG. 2) The effects of sucrose on the development of drosophilid flies show no inhibitory effects but does not exhibit an enhancing effect which was shown in the previous work. 3) The numbers of F_1 flies produced in the Sinchon-I strain are a little greater than in the Oregon-R. But this difference is not considerable as in the previous investigation. 4) The Muller-5 test shows a negative result, suggesting that MSG may be not mutagenic. Thus the results of the present experiment is roughly agreed with the previous one, confirming that MSG has an ill effect on the development of Drosophila melanogaster.

한국인 집단의 효소의 다형현상에 대한 연구 : 태반과 혈액에서의 MDH, LDH 그리고 GPI에 대하여 MDH, LDH and GPI in Placental and Blood

鄭瑢載,姜順子,金甲英 이화여자대학교 생명과학연구소 1990 생명과학연구논문집 Vol.1 No.-

한국인 집단의 태반조직과 혈액 내에서의 Malate dehydrogenase(MDH), Lactate dehydrogenase(LDH) alc Glucose phosphate isomerase(GPI)에 대한 다형현상을 알아 보고자 horizontal starchgel 전기영동법을 이용하여 조사한 결과는 다음과 같다. 1. MDH 동위 효소는 혈액에서는 , 한가지 표현형, HDHs1이 나타났으며, 태반에서는 SolubleMDH와 Mitochondial-MDH의 두 form이 모두 나타나는데 태반조직의 MDHs locus에서는 MDHs1 유형만이 나타났고 MDHs locus에서도 보편적 유형인MDHm1 표현형이 나타났다. 2. LDH 동위 효소는 표현형이 혈액에서 normal LDH isozyme인 LDH1, LDH2, LDH3, isozyme이 나타났고, 태반에서는 LDH1(LDHB4) isozyme 한가지 유형만 나타났다. 3. GPI 동위 효소는 혈액과 태반에서 표현형이 모두 같은 형인 GPI1으로 나타났으며 변이형은 한국인 집단에서 발견되지 않았다. 4. 따라서 한국인 집단의 혈액과 태반에서 MDH, LDH, GPI 효소는 모두 monomorphic한 것으로 나타났으나 LDH의 경우만은 혈액과 태반에서 검출된 isozyme의 종류가 상이하였다. Genetic polymorphisms of three placental and blood cell enzymes malate dehydrogenase(MDH),lactate dehydrogenase(LDH) and glucose phosphate isomerase(GPI) in Korean population was studied by starch gel electrophoresis. The results obtained were as follows: 1. MDH phenotype was found to be only MDHs 1 in blood cells. Both of MDHs and MDHm were found in the placental. Only MDHs 1 was found at the MDHs I locus. 2. LDH phenotype was found to be the normal LDH isozyme-LDH1, LDH2 and LDH3 isozyme in blood cells. And DH1(LDHB4) isozyme was found only in the placental extracts. 3. GPI phenotype was found to be only GPI 1 in blood cells and the placental extracts, and no variant type was ound in Korean population. 4. Based on the above results, MDH, LDH and GPI isozymes are monomorphic in blood cells and placental extracts of Korean population.

한국인 집단의 효소의 다형형상에 대한 연구 : 태반과 혈액에서의 MDH, LDH 그리고 GPI에 대하여 MDH, LDH and GPI in Placental and Blood

鄭瑢載,姜順子,金甲英 梨花女子大學校 韓國生活科學硏究院 1990 韓國生活科學硏究院 論叢 Vol.45 No.-

한국인 집단의 태반조직과 혈액 내에서의 Malate dehydrogenase(MDH), Lactate dehydrogenase(LDH) 및 Glucose phosphate isomerase(GPI)에 대한 다형현상을 알아 보고자 horizontal starch gel 전기영동법을 이용하여 조사한 결과는 다음과 같다. 1. MDH 동위 효소는 혈액에서는, 한가지 표현형, HDHs1이 나타났으며, 태반에서는 Soluble-MDH와 Mitochondrial-MDH의 두 form이 모두 나타나는데 태반조직의 MSHs locus에서는 MDHs1 유형만이 나타났고 MDHs locus에서도 보편적 유형인 MDHm1 표현형이 나타났다. 2. LDH 동위 효소는 효현형이 혈액에서 normal LDH isozyme인 LDH1, LDH2, LDH3, isozyme이 나타났고, 태반에서는 LDH1(LDHB4) isozyme 한가지 유형만 나타났다. 3. GPI 동위 효소는 혈액과 태반에서 표현형이 모두 같은 형인 GPI1으로 나타났으며 변이형은 한국인 집단에서 발견되지 않았다. 4. 따라서 한국인 집단의 혈액과 태반에서 MDH, LDH, GPI 효소는 모두 monomorphic한 것으로 나타났으나 LDH의 경우만은 혈액과 태반에서 검출된 isozyme의 종류가 상이하였다. Genetic polymorphisms of three placental and blood cell enzymes malate dehydrogenase(MDH), lactate dehydrogenase(LDH) and glucose phosphate isomerase(GPI) in Korean population was studied by starch gel electrophoresis. The results obtained were as follows : 1. MDH phenotype was found to be only MDH_2 1 in blood cells. Both of MDHs and MDHm were found in the placental. Only MDHs 1 was found at the MDHs I locus. 2. LDH phenotype was found to be the normal LDH isozyme-LDH1, LDH2 and LDH3 isozyme in blood cells. And LDH1(LDHB4) isozyme was found only in the placental extracts. 3. GP I phenotype was found to be only GP I1 in blood cells and the placental extracts, and no variant type was found in Korean population. 4. Based on the above results, MDH, LDH and GPI isozymes are monomorphic in blood cells and placental extracts of Korean population.

서울지역 식품영양전공·비전공대학생의 골밀도에 미치는 영향요인에 관한 연구

정남용,최순남 동아시아식생활학회 2003 동아시아식생활학회지 Vol.13 No.5

This study was conducted to investigate the factors affecting the bone density of food and nutrition major and non-major university students in Seoul area. Data for food habits, dietary and health-related behavior were obtained by self administered questionnaires. BQI(bone quality index) of the subjects were measured by Quantitative Ultrasound (QUS). The results are summarized as follows: The average height, weight, BMI and osteopenia percentage of the major and non-major male and female student were 174.49㎝, 67.05㎏, 21.96 and 22.0%, 174.34㎝, 65.98㎏, 21.69 and 11.8%; 160.76㎝, 54.48㎏, 21.07 and 40.0%; 161.30㎝, 54.22㎏, 20.84 and 40.2%, respectively. The BQI of the major and non-major subjects were 108.07 and 110.47 in male student group, and 89.13, 88.18 in female student group, respectively. The T-score and Z-score of bone density of the subjects were not significantly different. Weight and BMI were positively related with BQI in male and female group but the relationship with BMI tended to be stronger in non-major female group than other groups. BQI was positively affected by exercise time, favorite food, and intake of seafood and tea in major and non-major male student group. One-side eating habit and intake of instant foods were negatively related with BQI in both male groups. In major and non-major female student group, exercise time, meal regularity, favorite food, amount of meal, intake of tofu were related with BQI positively and intake of tea and/or meats negatively. The result of this study revealed that desirable food habits, dietary behavior and health-related lifestyle may have a beneficial effect on bone density. They need practically and systematically organized nutrition education on optimum body weight, good eating habits, weight bearing exercise and intakes of good quality nutrient for higher bone density level.

확률선형계획법에 적용한 안장함수의 기대치에 관한 연구

정순석,이용화 충주대 산업과학기술연구소 1999 産業科學論文集 Vol.7 No.1

This paper is organized as follows: Section 2 begins with a disscussion on existing bounds under first-order moments, and then presents the framework for developing new bounds under second-order moments. In section 3, this framework is used to derive lower and upper bounds for the expectation of a saddle function under simplicial domains, using first moments as well as variance/covariance information. Section 4 utilizes second-order joint moments and rectangular domauns to derive bounds for the saddle case. Section 5 give a example that are infimum and supremum of E[g] on convex-concave saddle function.

한국인 집단의 혈액과 태반에서의 Carbonic anhydrase와 Esterase D의 다형현상에 관한 연구

鄭瑢載,姜順子,金惠蘭 梨花女子大學校 韓國生活科學硏究院 1990 韓國生活科學硏究院 論叢 Vol.45 No.-

한국인 집단의 혈액과 태반 조직내에서의 carbonic anhydrase Ⅰ(CAⅠ), carbonic anhydraseⅡ(CAⅡ) 및 esterase D(EsD)효소에 대한 다형현상을 horizontal starch gel 전기영동법을 이용하여 조사한 결과는 다음과 같다. 1. 혈액에서는 CAⅠ과 CAⅡ 동위효소는 각각 CAⅠ1과 CAⅡ1유형의 한가지 표현형만을 나타냈으며, 변이형은 한국인 집단에서 발견되지 않았다. 2. EsD 동위효소는 혈액과 태반에서 모두 EsD*1과 EsD*2의 2개 대립유전자가 발견되었으며 변이형은 발견되지 않았다. 3. 적혈구에서의 EsD좌위의 대립유전자 빈도는 EsD*1은 0.661, EsD*2는 0.339로 나타났다. 4. 태반에서 나타나는 EsD의 유전자 빈도는 EsD*1과 EsD*2가 각각 0.664와 0.336으로 나타났다. 5. 위의 결과를 근거로 한국인 집단에서의 CAⅠ, CAⅡ효소는 monomorphic하며 EsD는 polymorphic함을 알 수 있다. Genetic polymorphism of the placental and red blood cell enzyme, carbonic anhydrase 1(CA1), carbonic anhydrase 11(CA11) and esterase D(EsD) in korean population were examined by means of horizontal starch gel electrophoresis. The results obtained were as follows : 1. Both of the CA1 and CA11 phenotypes were found to be only CAⅠ*1 and CAⅡ*1, respectively. No variant type was found in Korean population. 2. Two alleles, EsD*1 and EsD*2 were found in blood cell and placental extract, respectively no variant type was found. 3. The gene frequencies of EsD alleles in blood cell were calculated to be 0.661 for EsD^*1 and 0.339 for EsD^*2. 4. The gene frequencies of EsD alleles in placental extract were calculated to be 0.664 for EsD^*1 and 0.336 for EsD^*2. 5. Based on the above results, both CAⅠ and CAⅡ isozymes are monomorphic and EsD isozymes is polymorphic in Korean population.

소아의 연령별 Propionibacterium acnes에 대한 균체응집항체 및 동종혈구응집항체간의 비교연구

정화영,정상인,최철순,양용태 중앙대학교 의과대학 의과학연구소 1984 中央醫大誌 Vol.9 No.3

In general, the concentration of maternal IgG globulin in serum of the neonates falls rapidly within the first few months after birth and the production of IgM globulin develops in the maturing infants during the course of exposure to various antigens in the environment. The development of natural antibodies, ie., isohemagglutinins, IgM class, to ABO blood group substances and agglutinins, Ig class, to normal flora, in the early stage of life is important since not only they may act as bactericidal substances in nonspecific manners, but also could be immunological barometers on the normal function of humoral immune system. The high concentration of isohemagglutinins to human A or B blood group antigens and agglutinating antibodies to some of normal flora such as Propionibacterium acnes or Staphylococcus aureus were observed in normal human sera. It has been known that the serum concentration of IgM globulin usually reached adult levels by one year of age, while that of IgG globulins by five to six years of age. However, the levels of isohemagglutinins to A and B group substances and agglutinating to Propionibacterium acnes in children's sera and the ages in which the concentrations of their antibodies reached to abult levels are not clarified. In this study, the concentrations of isohemagglutinins to A and B blood group antigens and agglutinating antibodies to P.acnes serotype Ⅰ and serotype Ⅱ in the sera of 163 normal children, ranged from 0 day to 15 years of age, were measured by means of microtitration technique. The results obtained are as follows: 1. In the sera of 163 children under 15 years of age, there observed no significant difference in the titers of agglutinating antibodies to P. acnes serotype Ⅰ andⅡ. 2. Of 75 sera of children under one year of age, the numbers of sera in which agglutinating antibodies were not detectable or less than 1:4 to P. acnes serotype Ⅰ were 67(89.6%) and to serotype Ⅱ 53(70.7%), respectively. 3. Agglutinating antibody to P. acnes serotype Ⅰ in the children's sera reached adult levels by 7 years of age, but 100 percentages of antibody detection was observed only in the age group of 15 years old, whereas the adult levels of isohemagglutinins to A and B blood group antigens were observed in the age group of 6 months old. 4. No correlation were observed in normal children's sera between agglutinating antibody titre to P. acnes serotype Ⅰ and isohemagglutinin titre. These results indicated that the isohemagglutinins to ABO blood substances appeared in the earlier stage of life than did agglutinating antibodies to P. acnes.

환경행정공무원의 전문성과 환경행정의 규제성에 관한 조사연구 : 2001, 2003년 조사사례를 중심으로

정용택,박종안,손부순,이종화,장봉기,박정임 순천향대학교 기초과학연구소 2008 순천향자연과학연구 논문집 Vol.14 No.1

We have analyzed this influence base on survey of central government administration officers and local officers which were taken on 2001 and 2003. Also, we anticipates that this result would help actual field adjustment as well as preparation of speciality of officers. The results are summarized as follows : First, a survey in 2001 shows 68.8 percent of local administration officers answered that their expertise about environmental services were above average. Then, the result was increased to 82 percent of officers responsed that they have more than average expertise and experience in 2003 survey. Secondly, government have to increase effectiveness on regulation or restriction duty. Nonetheless, the survey shows that significant decrease of effectiveness from 2001 to 2003 in both central and local goveniment officers. It means that they spent more effort on local community environmental regulation program other than restriction duty to requisited from central government. Third, moreover, local government do not want to faculty of environmental administration and insists that governments need to set the regulation for particularity of regions.

韓國人 集團의 胎盤內 酵素의 遺傳的 多型現象

鄭瑢載,姜順子,韓美熙 梨花女子大學校 韓國生活科學硏究院 1989 韓國生活科學硏究院 論叢 Vol.44 No.-

한국인 집단의 효소다형현상을 알아보기 위하여 한국인 400개체의 태반효소 3가지 즉 acid α-glucosidase(GAA), superoxide dismutase(SOD_A) 및 NADH-diaphorase(DIA1)을 대상으로 horizontal starch gel 전기영동법으로 조사한 결과는 다음과 같다. 1. GAA와 SOD_A isozyme은 표현형이 각각 한가지 유형(GAA1과 SOD_A1)으로 나타나며 다른변이형은 발견되지 않았다. 2. DIA1 isozyme은 DIA1유형만 아니고 DIA1 2-1유형도 400개체 중 6 개체가 발견되었고 DIA1*1과 DIA1*2 인자빈도는 각각 0.9925와 0.0075로 나타났다. 3. 위의 결과로 볼 때 한국인 집단의 태반조직 내의 GAA와 SOD_A효소가 monorphicg하고 DIA1은 유전적 다형현상을 나타냄을 알 수 있다. Genetic polymorphism of three placental enzymes, acid α-glucosidase(GAA), superoxide dismutase(SOD_A) and NADH-diaphorase(DIA1) in Korean population was studied by starch-gel electrophoresis. The results were as follows: 1. Both of GAA and SOD_A phenotypes were only GAA1 and SOD_A1, respectively. No variant type was found in Korean population. 2. DIA1 isozyme was the common phenotype, DIA 1*1 and DIA 1*2 with about 6 in 400 Koreans. The gene frequencies of DIA1 alleles were 0.9925 for DIA 1*1 and 0.0075 for DIAI*2. 3. Based on the above results, both GAA and SOD_A isozymes were monomorphic and only DIA1 isozyme was polymorphic.

히야신스의 화경 기내배양시 자구의 재생과 생장에 미치는 생장조절제의 영향

정용모,김진희,이용문,이영병,이경순 東亞大學校附設遺傳工學硏究所 1997 遺傳工學硏究 Vol.- No.4

히야신스 3품종(Jan Bos, Pink Pearl, Carnegie)의 화경절편들을 조직배양을 통해 자구와 뿌리의 형성능력을 조사해 본 결과 품종에 따라 Murashige & Skoog의 기본 고체배지 또는 IAA 3 ?? + BA 0.1 ??, IAA 3 ?? + BA 1 mg ·L-1, NAA 0.1 ?? + BA 0.1 ??, 그리고 NAA 0.1 ?? + BA 1 mg· L-1의 혼용배지들에서 발근이 된 자구들을 바로 획득할 수 있었는데, 화경의 착화부 조직은 비착화부 조직에 비해 자구의 재생율이 높고 재생기간도 빨랐다. 반면에, 후자의 조직은 전자의 조직에 비해 발근율이 높고 발근기간도 훨씬 빠른 경향을 보였다. 그리고 발근이 된 자구들을 분리시켜 계대배양하는 과정에서 IAA 1 ?? + BA 0.1 ?? 또는 NAA 0.5 ?? + BA 0.1??의 혼용처리는 자구들의 생장을 대체로 촉진시켰지만, 뿌리 생장과 신초의 발육은 억제했다. 그리고 부정근의 형성이 용이치 않은 분리된 자구들은 무처리구에서 발근이 양호하였다. Tissue culture was applied to investigate the regeneration of bulblets on inflorescence stalk segments of Hyacinthus orientalis cvs. Jan Bos, Pink Pearl, and Carnegie. Bulblets with roots were obtained directly on Murashige and Skoog's medium or on basal medium supplemented with 3 ?? IAA + 0.1 ?? BA or 3 ?? IAA+1 ?? BA or 0.1 ?? NAA + 0.1 ?? BA or 0.1 ?? NAA + 1 ?? BA according to cultivars. And the bulblets regeneration ability of inflorescence stalk tissue with inflorescence was higher and the days required for the formation of bulblets was shorter than that of inflorescence satlk tissue without inflorescence, on the contrary, the root formation ability of the latter was higher and the days required for the formation of roots of the latter was much faster than the former. During the subcultures of seperate bulblets with roots, the bulblet growth was generatreatments of 1 ?? IAA + 0.1 ?? BA or 0.05 NAA + 0.1 ?? BA. And the root formation of seperate bulblets without adventitious roots was good on basal medium without growth substances.