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RISS 인기검색어
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- 저자 : Soo Jung Hyun(현수정) (검색결과 2 건)
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Analysis of Hybridization in Korean Ecotypes of Poa pratensis L. Using RAPD and SCAR Markers
Soo Jung Hyun(현수정),Sang Ryul Shim(심상렬),Byung Joon Ahn(안병준) 한국원예학회 2010 원예과학기술지 Vol.28 No.2
한국에는 켄터키블루그래스(왕포아풀, Poapratensis L.)의 다양한 생태형이 서식하고 있으며, 이들 중 일부는 잔디적 특성이 우수할 뿐만 아니라 높은 단위생식성을 나타내고 있다. 본 연구는 계통 특이적 DNA 표지를 개발하고 이를 이용하여 생태형들의 교배 효율을 분석하기 위하여 수행되었다. 생태형들과 육성종(Midnight, Unique, Challenger, Award, Blacksburg)들 간에 115조합의 교배를 수행하여 8,612 개체의 후대 식물을 얻었으며, 이로부터 지면피복률이 뛰어나고 엽색이 뛰어난 ‘2-73’과 엽밀도와 봄 green-up이 우수한 ‘31-40’ 계통을 선발하였다. 생태형 27종과 육성종 5종에 대한 RAPD 분석에서 OPB 프라이머(#4, 5, 7, 8, 10, 18)를 이용하여 20개의 계통 특이적 표지를 얻었으며, 이들 염기서열을 분석하여 SCAR 표지 14개를 개발하고 이들 표지의 조합으로 16종의 계통들을 식별할 수 있게 되었다. 또한 국내 생태형을 종자친으로하고 육성종을 화분친으로 교배하여 선발된 우수 계통 ‘2-73’과 ‘31-40’의 교배 여부를 교배 모본의 SCAR 표지를 통하여 확인할 수 있었다. Ecotypes of Poa pratensis that exhibit desirable traits as turfgrass such as fast proliferation for ground covering and environmental tolerances were collected in southern Korea. Like most typical varieties of Kentucky bluegrass, the ecotypes had varying levels of apomixis which resulted in low hybridization efficiency. Therefore, experiments were carried out to (1) develop genotype-specific RAPD and SCAR markers, and (2) to determine hybridization rates among the ecotypes and to aid the selection of desirable hybrids. Hybridization among the ecotypes and improved cultivars such as ‘Midnight’, ‘Unique’, ‘Challenger’, ‘Award’, and ‘Blacksburg’ were performed using an in vitro hybridization technique. From 115 crossing combinations among 47 ecotypes and improved cultivars, 8,612 progeny plants were obtained. Desirable progenies were identified in ‘2-73’ which was outstanding in ground covering from a seeded plug as well as its fine texture with darker blue leaf color, and ‘31-40’, which showed relatively early spring green-up. Random Amplified Polymorphic DNA (RAPD) profiles of 27 ecotypes and 5 improved cultivars were examined using OPB primers of 4, 5, 7, 8, 10 and 18, and twenty polymorphic markers were obtained. Sequences for six of the polymorphic RAPD bands were developed into Sequence Characterized Amplified Region (SCAR) markers. The SCAR markers were useful in determining crossing rates between the ecotypes and the improved cultivars used as pollen parents. They were also used to confirm the hybridization of selected progenies like ‘2-73’ (‘P77’ × ‘Midnight’) by the presence of a SCAR marker of the pollen parent.
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마르셀 뒤샹의 〈에탕 도네〉에 나타난 에로티시즘의 전략과 ‘앤드로지니’의 차용
현수정(Hyun Soo-jung) 현대미술사학회 2010 현대미술사연구 Vol.0 No.27
Marcel Duchamp(1887-1968) is one of the most influential artists of the twentieth century. The study of Duchamp's work has emerged as an important and controvetsial topic in art history. There have been two major streams of research on Duchamp's works: One is an inventor of the Dacia "ready- made", and the other is his influence on feminism through the alter-ego of "Rose Selavy". The topic of Duchamp as Rose Selavy is based on understanding the sexuality of Duchamp's work. This research is focusing on the second stream, but it does not strictly follow the theory of feminism. This study is an exploration of Duchamp's work by emphasizing yet another methodical possibility: strategy of eroticism and appropriation of Androgyny in his last work, 〈Etant donnes〉. What is the meaning of 'Androgyny'? It literally means a combining of two sexes or interplay of the opposites of 'masculinity' and 'feminist' in one figure. The term may also be used to represent the physical bisexuality of the hermaphrodite, that is a very limited and misunderstood usage of it The meaning of 'Androgyny' is not only from the physical concept but also from spiritual concept which is based on the ideal of religion and metaphysic. How could Duchamp get the idea of Androgyny in his work as an artist of 20th century? Though Duchamp didn't reveal how he got the idea, he acknowledged the influence of Alchemy. Duchamp developed his own way to appropriate images of Androgyny in his works. 〈Etant donnes〉(Given: 1. The Waterfall, 2. The illuminating Gas) is Duchamp's final masterwork which had been secretly constructed from 1946 to 1966. This work is three-dimensional assemblage, which offers an extraordinary shocking visual experience. Viewers are expected to look through a pair of peep holes on the wooden door which makes a block between inside and outside. The voyeur beholds a realistically constructed nude (naked body) lying with the spread legs on twigs, and holding a gas lamp in one hand The most controversial part of this work is the naked body. Though it is not a hermaphrodite, the sexual organs appear ambiguous. It is a symbolic figure represented as an androgynous body. Because of this, we should think beyond the physical version of the body to understand the artist's complex ideas. The clue to understand the work is in the title, 'Given: 1. The Waterfall, 2. The illuminating Gas'. 'Given' is a condition to be granted two elements, 'the waterfall and the illuminating Gas'. What do two elements symbolize? The title is at the preface of Duchamp's Green Box notes. That means 〈Etant donnes〉 is another version of 〈Large Glass〉. According to the connection of two works, the Waterfall is a symbol of female and the illuminating Gas is a symbol of male. The androgyne is at the center of the work. The two symbols support the central theme to make the balance of female and male. The condition of 〈Etant donnes〉 is two poles in this work, one is the naked body of the inside and the other is viewers of the outside. He strongly revealed eroticism in this work, but in the form of Androgyny as an allegorical appearance. His idea of Androgyny became conceptualized. There is Duchamp's play and intrigue in this work, he concealed the essential meaning beyond sexuality. The work reveals an image of conceptual ambiguity. The appropriation of Androgyny is represented by the reconciliation of opposition between art works and viewer. That is less a conclusion than an open-ended investigation. He didn't have any intention about postmodernism, but the idea of appropriation in his works is one of the most important strategies of postmodernism. The study of Androgyny in Duchamp gives a new version to understand Duchamp in the continuity of art history.
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