Integrated analysis of quantitative proteome and transcriptional profiles reveals abnormal gene expression and signal pathway in bladder cancer

Songbai Liao,Minglin Ou,Liusheng Lai,Hua Lin,Yaoshuang Zou,Yonggang Yu,Xuede Li,Yong Dai,Weiguo Sui 한국유전학회 2019 Genes & Genomics Vol.41 No.12

Background Bladder cancer (BCa) is a tumor associated with high morbidity and mortality and its incidence is increasing worldwide. However, the pathogenesis of bladder cancer is not well understood. Objective To further illustrate the molecular mechanisms involved in the pathogenesis of BCa and identify potential therapeutic targets, we combined the transcriptomic analysis with RNA sequencing and tandem mass tags (TMT)-based proteomic methods to quantitatively screen the differentially expressed genes and proteins between bladder cancer tissues (BC) and adjacent normal tissues (AN). Results Transcriptome and proteome studies indicated 7094 differentially expressed genes (DEGs) and 596 differentially expressed proteins (DEPs) between BC and AN, respectively. GO enrichment analyses revealed that cell adhesion, calcium ion transport, and regulation of ATPase activity were highly enriched in BCa. Moreover, several key signaling pathway were identified as of relevance to BCa, in particular the ECM-receptor interaction, cell adhesion molecules (CAMs), and PPAR signaling pathway. Interestingly, 367 genes were shared by DEGs and DEPs, and a significant positive correlation between mRNA and translation profiles was found. Conclusion In summary, this joint analysis of transcript and protein profiles provides a comprehensive reference map of gene activity regarding the disease status of BCa.

An experimental study on pool sloshing behavior with solid particles

Songbai Cheng,Shuo Li,Kejia Li,Ting Zhang 한국원자력학회 2019 Nuclear Engineering and Technology Vol.51 No.1

It is important to clarify the mechanisms of molten-fuel-pool sloshing behavior that might be encounteredduring a core disruptive accident of sodium-cooled fast reactors. In this study, motivated byacquiring some evidence for understanding the characteristics of this behavior at more realistic conditions,a number of experiments are newly performed by injecting nitrogen gas into a water pool with theaccumulation of solid particles. To achieve comprehensive understanding, various parameters includingparticle bed height, particle size, density, shape, gas pressure along with the gas-injection duration, wereemployed. It is found that due to the different interaction mechanisms between solid particles and thegas bubble injected, three kinds of regimes, termed respectively as the bubble-impulsion dominantregime, the transitional regime and the bed-inertia dominant regime, could be identified. The performedanalyses also suggest that under present conditions, all our experimental parameters employed can havenoticeable impact on the regime transition and resultant sloshing intensity (e.g. maximum elevation ofwater level at pool peripheries). Knowledge and fundamental data from this work will be used for thefuture verifications of fast reactor severe accident codes in China

Investigation of fl ow regime in debris bed formation behavior with nonspherical particles

Songbai Cheng,Pengfeng Gong,Shixian Wang,Jinjiang Cui,Yujia Qian,Ting Zhang,Guangyu Jiang 한국원자력학회 2018 Nuclear Engineering and Technology Vol.50 No.1

It is important to clarify the characteristics of flow regimes underlying the debris bed formation behaviorthat might be encountered in core disruptive accidents of sodium-cooled fast reactors. Although in ourprevious publications, by applying dimensional analysis technique, an empirical model, with itsreasonability confirmed over a variety of parametric conditions, has been successfully developed topredict the regime transition and final bed geometry formed, so far this model is restricted to predictionsof debris mixtures composed of spherical particles. Focusing on this aspect, in this study a new series ofexperiments using nonspherical particles have been conducted. Based on the knowledge and data obtained,an extension scheme is suggested with the purpose of extending the base model to cover theparticle-shape influence. Through detailed analyses and given our current range of experimental conditions,it is found that, by coupling the base model with this scheme, respectable agreement betweenexperiments and model predictions for the regime transition can be achieved for both spherical andnonspherical particles. Knowledge and evidence from our work might be utilized for the futureimprovement of design of an in-vessel core catcher as well as the development and verification of sodium-cooled fast reactor severe accident analysis codes in China.

EFFICIENT PRODUCTION OF BIO-GASOLINE FROM SUGAR POLYOLS OVER RU-MOLYBDENUM OXIDE CATALYSTS

Songbai Qiu,Yujing Weng,Lungang Chen,Longlong Ma,Qi Zhang,Tiejun Wang 한국신재생에너지학회 2015 AFORE Vol.2015 No.11

Characteristics of Self-Leveling Behavior of Debris Beds in a Series of Experiments

Songbai Cheng,Hidemasa Yamano,Tohru Suzuki,Yoshiharu Tobita,Yuya Nakamura,Bin Zhang,Tatsuya Matsumoto,Koju Morita 한국원자력학회 2013 Nuclear Engineering and Technology Vol.45 No.3

During a hypothetical core-disruptive accident (CDA) in a sodium-cooled fast reactor (SFR), degraded core materials can form roughly conically-shaped debris beds over the core-support structure and/or in the lower inlet plenum of the reactor vessel from rapid quenching and fragmentation of the core material pool. However, coolant boiling may ultimately lead to leveling of the debris bed, which is crucial to the relocation of the molten core and heat-removal capability of the debris bed. To clarify the mechanisms underlying this self-leveling behavior, a large number of experiments were performed within a variety of conditions in recent years, under the constructive collaboration between the Japan Atomic Energy Agency (JAEA)and Kyushu University (Japan). The present contribution synthesizes and gives detailed comparative analyses of those experiments. Effects of various experimental parameters that may have potential influence on the leveling process, such as boiling mode, particle size, particle density, particle shape, bubbling rate, water depth and column geometry, were investigated, thus giving a large palette of favorable data for the better understanding of CDAs, and improved verifications of computer models developed in advanced fast reactor safety analysis codes.

Investigation of flow-regime characteristics in a sloshing pool with mixed-size solid particles

Cheng, Songbai,Jin, Wenhui,Qin, Yitong,Zeng, Xiangchu,Wen, Junlang Korean Nuclear Society 2020 Nuclear Engineering and Technology Vol.52 No.5

To ascertain the characteristics of pool sloshing behavior that might be encountered during a core disruptive accident of sodium-cooled fast reactors, in our earlier work several series of experiments were conducted under various scenarios including the condition with mono-sized solid particles. It is found that under the particle-bed condition, three typical flow regimes (namely the bubble-impulsion dominant regime, the transitional regime and the bed-inertia dominant regime) could be identified and a flow-regime model (base model) has been even successfully established to estimate the regime transition. In this study, aimed to further understand this behavior at more realistic particle-bed conditions, a series of simulated experiments is newly carried out using mixed-size particles. Through analyses, it is verified that for present scenario, by applying the area mean diameter, our previously-developed base model can provide the most appropriate predictive results among the various effective diameters. To predict the regime transition with a form of extension scheme, a correction factor which is based on the volume-mean diameter and the degree of convergence in particle-size distribution is suggested and validated. The conducted analyses in this work also indicate that under certain conditions, the potential separation between different particle components might exist during the sloshing process.

CHARACTERISTICS OF SELF-LEVELING BEHAVIOR OF DEBRIS BEDS IN A SERIES OF EXPERIMENTS

Cheng, Songbai,Yamano, Hidemasa,Suzuki, TYohru,Tobita, Yoshiharu,Nakamura, Yuya,Zhang, Bin,Matsumoto, Tatsuya,Morita, Koji Korean Nuclear Society 2013 Nuclear Engineering and Technology Vol.45 No.3

During a hypothetical core-disruptive accident (CDA) in a sodium-cooled fast reactor (SFR), degraded core materials can form roughly conically-shaped debris beds over the core-support structure and/or in the lower inlet plenum of the reactor vessel from rapid quenching and fragmentation of the core material pool. However, coolant boiling may ultimately lead to leveling of the debris bed, which is crucial to the relocation of the molten core and heat-removal capability of the debris bed. To clarify the mechanisms underlying this self-leveling behavior, a large number of experiments were performed within a variety of conditions in recent years, under the constructive collaboration between the Japan Atomic Energy Agency (JAEA) and Kyushu University (Japan). The present contribution synthesizes and gives detailed comparative analyses of those experiments. Effects of various experimental parameters that may have potential influence on the leveling process, such as boiling mode, particle size, particle density, particle shape, bubbling rate, water depth and column geometry, were investigated, thus giving a large palette of favorable data for the better understanding of CDAs, and improved verifications of computer models developed in advanced fast reactor safety analysis codes.

Knowledge from recent investigations on sloshing motion in a liquid pool with solid particles for severe accident analyses of sodium-cooled fast reactor

Ruicong Xu,Songbai Cheng,Shuo Li,Hui Cheng 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.2

Investigations on the molten-pool sloshing behavior are of essential value for improving nuclear safetyevaluation of Core Disruptive Accidents (CDA) that would be possibly encountered for Sodium-cooledFast Reactors (SFR). This paper is aimed at synthesizing the knowledge from our recent studies onmolten-pool sloshing behavior with solid particles conducted at the Sun Yat-sen University. To bettervisualize and clarify the mechanism and characteristics of sloshing induced by local Fuel-CoolantInteraction (FCI), experiments were performed with various parameters by injecting nitrogen gas intoa 2-dimensional liquid pool with accumulated solid particles. It was confirmed that under differentparticle-bed conditions, three representative flow regimes (i.e. the bubble-impulsion dominant, transitional and bed-inertia dominant regimes) are identifiable. Aimed at predicting the regime transitionsduring sloshing process, a predictive empirical model along with a regime map was proposed on thebasis of experiments using single-sized spherical solid particles, and then was extended for coveringmore complex particle conditions (e.g. non-spherical, mixed-sized and mixed-density spherical particleconditions). To obtain more comprehensive understandings and verify the applicability and reliability ofthe predictive model under more realistic conditions (e.g. large-scale 3-dimensional condition), furtherexperimental and modeling studies are also being prepared under other more complicated actualconditions

Impact of Isovalent and Aliovalent Doping on Mechanical Properties of Mixed Phase BiFeO₃

Heo, Yooun,Hu, Songbai,Sharma, Pankaj,Kim, Kwang-Eun,Jang, Byung-Kweon,Cazorla, Claudio,Yang, Chan-Ho,Seidel, Jan American Chemical Society 2017 ACS NANO Vol.11 No.3

<P>In this study, we report the effect of doping in morphotropic BiFeO3 (BFO) thin films on mechanical properties, revealing variations in the elasticity across the competing phases and their boundaries. Spectroscopic force-distance (F-D) curves and force mapping images by AFM are used to characterize the structure and elastic properties of three BFO thin-film candidates (pure-BFO, Ca-doped BFO, La-doped BFO). We show that softening behavior is observed in isovalent La-doped BFO, whereas hardening is seen in aliovalent Ca-doped BFO. Furthermore, quantitative F-D measurements are extended to show threshold strengths for phase transitions, revealing their dependence on doping in the system. First-principles simulation methods are also employed to understand the observed mechanical properties in pure and doped BFO thin films and to provide microscopic insight on them. These results provide key insight into doping as an effective control parameter to tune nanomechanical properties and suggest an alternative framework to control coupled ferroic functionalities at the nanoscale.</P>

Thrombospondin-2 Couples Pressure-Promoted Chondrogenesis through NF-κB Signaling

Niu Jing,Feng Fan,Zhang Songbai,Zhu Yue,Song Runfang,Li Junrong,Zhao Liang,Wang Hui,Zhao Ying,Zhang Min 한국조직공학과 재생의학회 2023 조직공학과 재생의학 Vol.20 No.5

BACKGROUND: Our previous studies found that the mechanical stimulation promote chondrogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), along with up-regulation of thrombospondin-2 (TSP-2). The aim of this study was to explore the effect of thrombospondin-2 (TSP-2) on the mechanical pressure-stimulated chondrogenic differentiation of BMSCs and the possible role of NF-jB signaling in the mechano-chemical coupling regulation toward chondrogenesis. METHODS: Rat BMSCs were isolated, cultured and identified. The time-dependent expressions of TSP-2 and Sox9 in BMSCs under a dynamic mechanical pressure of 0–120 kPa at 0.1 Hz for 1 h were tested by qPCR and Western blotting. The role of TSP-2 in chondrogenic differentiation of BMSCs under mechanical pressure was validated by using small interfering RNA. The impact of TSP-2 and mechanical pressure on chondrogenesis were detected and the downstream signaling molecules were explored using Western blotting. RESULTS: Mechanical pressure stimulation of 0–120 kPa for 1 h significantly upregulated the expression of TSP-2 in BMSCs. The expression of the chondrogenesis markers Sox9, Aggrecan, and Col-II were all upregulated under dynamic mechanical pressure or TSP-2 stimulation. Additional exogenous TSP-2 may potentiate the chondrogenic effect of mechanical stimulation. After knock down TSP-2, the upregulation of Sox9, Aggrecan and Col-II under mechanical pressure was inhibited. The NF-jB signaling pathway responded to both dynamic pressure and TSP-2 stimulation, and the cartilage-promoting effect was blocked by an NF-jB signaling inhibitor. CONCLUSION: TSP-2 plays an essential role in the chondrogenic differentiation of BMSCs under mechanical pressure. NF-jB signaling is involved in the mechano-chemical coupling of TSP-2 andmechanical pressure for the chondrogenic differentiation of BMSCs.