Surgical Management of Coronary Artery Fistulas in Children

Song Youngkwan,Choi Eun Seok,Kim Dong-Hee,Kwon Bo Sang,Park Chun Soo,Yun Tae-Jin 대한심장혈관흉부외과학회 2024 Journal of Chest Surgery (J Chest Surg) Vol.57 No.1

Background: This study investigated the surgical outcomes associated with coronary artery fistulas (CAFs) in children. Methods: We retrospectively reviewed the medical records of 23 pediatric patients who underwent surgical closure of CAFs between 1995 and 2021. At presentation, 7 patients (30.4%) exhibited symptoms. Associated cardiac anomalies were present in 8 patients. Fourteen fistulas originated from the right coronary artery and 9 from the left. The most common drainage site was the right ventricle, followed by the right atrium and the left ventricle. The median follow-up duration was 9.3 years (range, 0.1–25.6 years) Results: The median age and body weight at repair were 3.1 years (range, 0–13.4 years) and 14.4 kg (range, 3.1–42.2 kg), respectively. Cardiopulmonary bypass was used in 17 cases (73.9%), while cardioplegic arrest was employed in 14 (60.9%). Epicardial CAF ligation was utilized in 10 patients (43.5%), the transcoronary approach in 9 (39.1%), the endocardial approach in 2 (8.7%), and other methods in 2 patients (8.7%). The application of cardioplegic arrest during repair did not significantly impact the duration of postoperative intensive care unit stay or overall hospital stay. One in-hospital death and 1 late death were recorded. The overall survival rate was 95.7% at 10 years and 83.7% at 15 years. A residual fistula was detected in 1 patient. During the follow-up period, no surviving patient experienced cardiovascular symptoms or coronary events. Conclusion: Surgical repair of CAF can be performed safely with or without cardioplegic arrest, and it is associated with a favorable prognosis in children.

가족체계와 여가유형에 따른 청소년의 가족여가 만족도

이영관(YoungKwan Lee),송진숙(JinSuk Song) 한국생활과학회 2007 한국생활과학회지 Vol.16 No.4

대전지역 중학생이 지각한 가족의 건강성 및 가족여가 태도에 따른 청소년의 가족여가 만족도

송진숙(JinSuk Song),이영관(YoungKwan Lee) 한국가정과교육학회 2007 한국가정과교육학회지 Vol.19 No.2

본 연구는 대전광역시 2곳의 중학교에 다니는 1학년과 2학년의 200명을 대상으로 하였고, 분석에 사용된 통계방법은 SPSS 통계프로그램으로 빈도, 백분율, t검증, 일원변량분석, 상관계수, 중다회귀분석, 단계적 회귀분석을 실시하였다. 가족의 건강성과 가족여가 태도가 청소년들의 가족여가만족도에 미치는 영향요인을 분석한 결과 첫째, 성별에 따른 가족여가 만족도는 여학생이 남학생보다 높게 나타났다. 둘째, 가정의 소득수준이 높을 때, 가족여가 만족도가 높은 것으로 나타났다. 셋째, 가족여가 태도와 가족여가 만족도는 관련성이 있는 것으로 나타났다. 넷째, 가족의 건강성과 가족여가 만족도는 관련성이 있는 것으로 나타났다. 다섯째, 가족의 여가 만족도를 가장 잘 설명하는 변인은 가족의 여가태도 중 감정적 태도로 나타났다. 그러므로 가족여가 만족도를 높이기 위해서는 가족구성원들이 가족여가의 필요성과 효과를 인식하고 실천하는 것이 가장 중요하다고 본다. The purpose of this study was to analyze a casual relationship of satisfaction of family leisure in middle school students and related variables(student s gender, parent s income, father s job, healthy family, and family leisure attitude). Subjects were 200 middle school students. Instrumentation were questionnaires from the family leisure satisfaction scale, healthy family scale, and family leisure attitude scale. Data analysis was by mean, percentile, t-test, oneway-ANOVA, Pearson correlation, multiple regression, and stepwise multiple regression. The major findings of this study were as follow; First there was a significant different between student s gender and satisfaction of family leisure. Second, there was a significant different between family income and satisfaction of family leisure. Third, there was a significant relationship between healthy family and satisfaction of family leisure. Fourth, there was a significant relationship between family leisure attitude and satisfaction of family leisure. Fifth, emotional attitude in family leisure attitude was the most predicting variable in satisfaction of family leisure.

Expression, glycosylation, and function of anti-colorectal cancer monoclonal antibody CO17-1A in insect cells

Eun-Yi Oh,Mira Song,Mi-Hyun Ahn,Da-Young Park,Kyung-Jin Lee,Yangkang So,Zhe Lu,Kwang-Wok Min,Seul-Ki Lee,Jung-Hwan Lee,Doo-Byoung Oh,Youngkwan Kim,Kisung Ko 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1

Insect cell expression system using baculovirus has several benefits from high capacity, flexibility, and safety to humans and glycosylation capability. Thus, the baculovirus insect cell system has been widely used for production of recombinant protein. The HC and LC genes of the mAb CO17-1A were cloned under the control of two different promoters, P10 and PPH, respectively, on baculovirus expression pFastBacTM Dual vector. The gene expression cassettes carrying HC and LC genes were transferred into a parent bacmid in DH10Bac. Immunoflouorescence analyses and Western blot confirmed expression and secretion of mAb CO17-1A in the virus infected insect cells. The optimum condition for mAb expression was optimized at 24, 48 and 72hr after the virus infection with MOI ranging (0.2, 1 and 5). HPLC analysis revealed that the insect-derived mAb CO17-1A had insect specific glycan structures. Cell ELISA showed that the purified mAb from insect cell cultured media had a specific binding activity to SW948 cell. These results indicated that the baculovirus insect cell system is able to express, assemble, and secrete functional full size monoclonal antibody with insect specific glycosylation.

Effect of ER signal peptides (KDEL) on expression and function of monoclonal antibody in plant

Da-Young Park,Eun-Yi Oh,Zhe Lu,Yangkang So,Mi-Hyun Ahn,Mira Song,Kyung-Jin Lee,Doo-Byoung Oh,Youngkwan Kim,Kisung Ko 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1

Plant genetic engineering led to the production of plant-derived mAb (mAbP), which provides a safe and economically feasible alternative to the current animal expression systems. In this study, the expression level of mAbP SO57 between with and without Lys-Asp-Glu-Leu (KDEL) was compared in transgenic plant. PCR and PT-PCR analyse showed stable gene insertion transcription of heavy and light chain genes of mAbP SO57 with or without KDEL in plant, respectively. We validated expression of mAbP SO57 by western blot. Western blot showed the significantly higher expression level of mAbP SO57 with KDEL compared to without KDEL. Flow cytometry (FACS) analysis showed that the Fc domains of both purified mAbP and mammalian-derived mAb (mAbM) evidenced similar binding activity to the FcγRI receptor (CD64). High performance liquid chromatography (HPLC) analysed, glycosylation patterns of mAbP SO57 with or without KDEL. The mAbP SO57 with KDEL had glycan profile with both oligomannose type (47.6%) and golgi type (52.4%), while the mAbP SO57 without KDEL had only golgi type (100%) glycans. Neutralizing analysis with rabies virus CVS-11 showed the similar neutralizing activity between mAbP SO57 with and without KDEL. These results suggest the potential of mAbP SO57 for rabies immunotherapy, regardless of plant specific glycan structures.

Expression of Anti-Colorectal Cancer Immunotherapeutic Monoclonal Antibody in Transgenic Plant

Yangkang So,Zhe Lu,Eun-Yi Oh,Da-Young Park,Kyung-Jin Lee,Mira Song,Seung Han Oh,Arshad Jamal,Mi-Hyun Ahn,Yeon Soo Han,Doo-Byoung Oh,Youngkwan Kim,Kisung Ko 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1

The mAb CO17-1A recognizes the colorectal antigen GA733-2E, which highly is expressed on the surface membrane of human colorectal carcinoma cells. We have successfully developed plant system for production of anti-colorectal cancer monoclonal antibody, mAb CO17-1A. Plant expression system for mAb production has several advantages in terms of the lack of animal pathogenic contaminants, low cost, ease of scale-up for agricultural production, and efficient post-translational glycosylation modifications compared to the conventional fermentation methods. KDEL (Lys-Asp-Glu-Leu), ER retention signal attached to C-terminus of protein might induce high protein expression and the glycoprotein carrying oligomannose glycans. In this study, the expression level of mAb CO17-1A was compared between mAbs fused with and without KDEL in plant. PCR analysis showed stable gene insertion of heavy and light chain genes of mAb CO17-1A with or without KDEL. We validated the expression of mAb CO17-1A by western blot and its subcellular localization under the confocal microscopy. Western blot showed the significantly increased expression level of mAb CO17-1A with KDEL compared to without KDEL. Cell ELISA with human colorectal carcinoma cell line confirmed that expression of the mAb with KDEL was significantly higher than that of mAb without KDEL. The glycosylation analysis revealed that mAb CO17-1A without KDEL had plant specific glycans structure while mAb CO17-1A with KDEL had mainly oligomannose type glycans. Taken together, these studies suggest that KDEL affects expression of mAb and its glycan structures.

Expression of Anti-Colorectal Cancer Monoclonal Antibody CO17-1A in Insect Cells

Eun-Yi Oh,Kyung-Jin Lee,Da-young Park,Yangkang So,Zhe Lu,Mira song,Mi-Hyun Ahn,Doo-Byoung Oh,Youngkwan Kim,Kisung Ko 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1

Insect cell expression system using baculovirus has several benefits from high capacity, flexibility, safety to humans and glycosylation capability. Thus, the baculovirus insect cell system has been widely used for production of recombinant protein. In this study, the insect cell expression system was designed to produce anti-cancer mAb CO17-1A, which recognizes the antigen GA733 highly expressed on the surface membrane of colorectal carcinoma cells. The heavy (HC) and light chain (LC) genes of the mAb CO17-1A were cloned under the control of two different promoters, P10 and PPH, respectively, on baculovirus expression pFastBacTM Dual vector. The gene expression cassettes carrying HC and LC genes were transferred into a parent bacmid in E.coli (DH10Bac). The bacmid was transfected to Sf9 insect cells to generate the baculovirus expressing mAb CO17-1A. Western blot and immunoflouorescence analyses confirmed expression and secretion of mAb CO17-1A in the virus infected insect cells. The optimum condition for mAb expression was optimized at 24, 48 and 72hr after the virus infection with MOI (optimum virus concentration) ranging (0.2, 1 and 5). Expression of mAb CO17-1A in insect cells significantly increased at both 48 and 72hr after transfection with the MOI 1. HPLC chromatography revealed that the insect-derived mAb CO17-1A had insect specific glycan structures. Cell ELISA showed that the purified mAb from insect cell cultured media had a specific binding activity to SW948 human colorectal cancer cell. These results indicated that the baculovirus insect cell system is able to express, assemble, and secrete functional full size monoclonal antibody with insect specific glycosylation.

Effect of ER signal peptides (KDEL) on expression and function of anti-virus monoclonal antibody in plant

Da-Young Park,Yangkang So,Kyung-Jin Lee,Zhe Lu,Eun-Yi Oh,Kwang-Wok Min,Seul-Ki Lee,Jung-Hwan Lee,Mi-Hyun Ahn,Mira Song,Doo-Byoung Oh,Youngkwan Kim,Kisung Ko 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1

Plant genetic engineering has led to the production of plant-derived mAb (mAbP), which provides a safe and economically feasible alternative to the current antibody expression systems. In this study, the expression levels of mAbP SO57 with or without ER-signal peptides (Lys-Asp-Gly-Leu;KDEL) in transgenic tobacco plants were analysed in transgenic plant. PCR and Reverse Transcription-PCR analyses showed existence of heavy and light chain genes of mAb with or without KEDL and their transcription in plant, respectively. Western blot showed that the expression levels of mAbP SO57 with KDEL were significantly higher than that without KDEL. Flow cytometry analysis showed that the Fc domains of both purified mAbP and mammalian-derived mAb have similar binding activity to the FcγRI receptor. High-performance liquid chromatography analysis showed that the mAbP SO57 with KDEL had glycan profile with both oligomannose and golgi type, whereas the mAbP SO57 without KDEL had only golgi type glycans. Neutralizing analysis with rabies virus CVS-11 showed the similar neutralizing activity between mAbP SO57 with and without KDEL. These results suggest that the potential of mAbP SO57 for rabies immunotherapy is regardless of plant specific glycan structures.

Glycomodification and charaterization of anti-colorectal cancer immunotherapeutic monoclonal antibody in plant

Yangkang So,Seul-Ki Lee,Da-Young Park,Zhe Lu,Eun-Yi Oh,Kwang-Wook Min,Jung-Hwan Lee,Mi-Hyun Ahn,Mira Song,Kyung-Jin Lee,Doo-Byoung Oh,Youngkwan Kim,Kisung Ko 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1

The mAb CO17-1A recognizes the colorectal antigen GA733-2E, which highly is expressed on the surface membrane of human colorectal carcinoma cells. The higher yield of CO17-1A mAb reflect the use of the ER retrieval motif KDEL(Lys-Asp-Glu-Leu), In this study, the expression level of mAb CO17-1A was compared between mAbs fused with and without KDEL in plant. Western blot showed that the expression levels of mAb CO17-1A with KDEL were significantly higher than that without KDEL Cell ELISA with human colorectal carcinoma cell line confirmed that expression of the mAb with KDEL was significantly higher than that of mAb without KDEL. Flow cytometry analysis showed that the Fc domains of both purified mAbP (with and without KDEL) and mammalian-derived mAb have similar binding activity to the FcγRI receptor. The glycosylation analysis revealed that mAb CO17-1A without KDEL had plant specific glycans structure while mAb CO17-1A with KDEL had mainly oligomannose type glycans. Taken together, these studies suggest that KDEL affects expression of mAb and its glycan structures.