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한국 금잔디(Zoysia Japonica Steud)의 콜히친 처리에 의한 돌연변이유기
임용표,Skogley, Conrad R,Yeam, Do Yi 한국유전학회 1990 Genes & Genomics Vol.12 No.3
Colchicine-induced mutants of Zoysia japonica STEUD here investigated to compare morphological and cytological characters. The pregerminated seeds were treated with colchicine in the combination of various concentrations and soaking durations. From the treatment, nine mutants, different from another in morphology, were selected. The diploid and tetraploid gametes were observed together in the colchicine-treated clone 11-4. When the twenty five subclones isolated from the node of mixoploid clone 11-4 were transplanted, the significant variations were observed in the cotton hair number, stomata number, internode length, rhizome diameter, leaf width, and total rhizome length. It seemed that these morphological variations were related to the various polyploid levels as tetraploid, octaploid, or mixoploid. From these studies, it has been shown that colchcine treatment significantly increases the genetic variability in Zoysiagrass, and that chlchicine be a valuable agent for induction of genetic variability in Zoysia genetic and breeding programs.
A Simple Spectrophotometric Determination of Nitrate in Water.Resin, and Soil Extract
Yang, J.E.,Skogley, E.O. Shaff, B.E.,Kim, J.J. 江原大學校 附設 環境硏究所 1998 環境硏究 Vol.15 No.-
Nitrate is one of the most important chemicals in agricultural and environmental management. Most methods for NO_(3) analysis. however, require expensive equipment or compliccated procdures. The objective of this study was to develop a simple rapid accourate procedure for NO_(3) analysis that can be conducted in laberatories worldwide whithout the need for specialized equipment We vased our studies on a colorimetric method that involves electrophilic aromatic substitution(nitration) between nitronium and salicylate. The simpligied procedure was tested for NO_(3) analysis of water, soil, and resin extracts. Only 1 ml. of sample containing 0 to 4 mg NO_(3)-N I is required and a single solution (TRI solution made as a mixture of sodium salicylate 2-hydrocybenzoic acid monosodium salt, NaCl and anmonium sulfamate sulfamic acid monoammonium salt is used for color develpment, The detection limit is 0.1㎍NO_(3)-N with ilnearity up to 4㎍ NO_(3) in a final volume of 11 ml Evuporation of the sample may be done in serveral wats to remove water fromreactants as long as the temperature for requried neutralization with 2 M Na()ll prior to analysis and soil extracts from 1M KCl provided more uniform results than from 2 M KCl Results from all types of solution samples were highly signigicatly correlated wuth those by the automated Cd-reduction method. This simple reproducible and it requires only inexpensive equipment.