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류시환 ( Si Hwan Ryu ),최재근 ( Jae Keun Choi ),김문종 ( Moon Jong Kim ),한정헌 ( Jeong Heon Han ),왕승현 ( Seung Hyun Wang ),김희연 ( Hee Yeon Kim ),김기선 ( Ki Sun Kim ),남궁민 ( Min Namgung ),박종열 ( Jong Yeol Park ),박기진 ( 강원대학교 농업생명과학연구원 2022 강원 농업생명환경연구 Vol.34 No.0
The main procedures of maize breeding are inbred line development and hybrid selection. Inbred line development is an essential procedure for the creation of good hybrids, but in South Korea, inbred line development is completely dependent on conventional breeding methods. This conventional inbred line development system is a tedious and time-consuming procedure. Doubled haploid technology in maize is a rapid inbred line development method and many foreign maize research institutes are actively using this technology. In this study, we aimed to set up and utilize doubled haploid technology in our breeding program. Inducer lines, which can produce haploid seeds, are a prerequisite for doubled haploid breeding. The Maize Research Institute secured the right to use tropically adapted haploid inducer lines (TAILs). The number of days to silking for TAILs was 72 and the flowering time was appropriate for crossing with other populations. When 48 maize populations were crossed with the inducer, the average haploid induction rate was 6.0%. Haploid induction rate for 2014 through 2017 were 3.9, 4.8, 6.7, and 8.3%, respectively. When we compare the chromosome doubling stage, the inbred line production rate of seedlings was higher than that of germinated seeds. Agronomic characteristics of inbred lines developed by doubled haploid technology were tested and those lines will be good resources as parent plants for making hybrids. Future work will aim to increase induction rate and the selection efficiency of haploid seeds, but our breeding efficiency and capacity in South Korea will be greatly improved by this technology.
용매열처리에 따른 PEDOT:PSS 암모니아 가스 감지막 특성 변화
노왕규 ( Wang Gyu Noh ),염세혁 ( Se-hyuk Yeom ),이왕훈 ( Wanghoon Lee ),신한재 ( Han Jae Shin ),계지원 ( Ji Won Kye ),곽기섭 ( Giseop Kwak ),김세현 ( Se Hyun Kim ),류시옥 ( Si Ok Ryu ),한동철 ( Dong Cheul Han ) 한국센서학회 2017 센서학회지 Vol.26 No.2
Poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) has been extensively studied as the active material in ammonia gas sensor because of its fast response time, high conductivity and environmental stability. It is well known that a post annealing process for organic devices based on PEDOT:PSS significantly increases the device performance. In this study, we propose the solvent annealing of PEDOT:PSS and investigated its effects. As a results, post solvent annealing on PEDOT:PSS lead to the surface chemical and physical properties change. These changes result in improved conductivity of the PEDOT:PSS. In additional, ammonia sensitivity of solvent annealed PEDOT:PSS become higher than pristine polymer film. The enhancement is mainly caused by the depletion of gas barrier PSS and structural re-forming PEDOT networks. We believe that the post solvent annealing is a promising method to achieve highly sensitivity PEDOT:PSS films for applications in efficient, low-cost and flexible ammonia gas sensor.
Occurrence, evolution, and functions of DNA phosphorothioate epigenetics in bacteria
Tong, Tong,Chen, Si,Wang, Lianrong,Tang, You,Ryu, Jae Yong,Jiang, Susu,Wu, Xiaolin,Chen, Chao,Luo, Jie,Deng, Zixin,Li, Zhiqiang,Lee, Sang Yup,Chen, Shi National Academy of Sciences 2018 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.115 No.13
<P><B>Significance</B></P><P>Phosphorothioate (PT) modification of the DNA sugar-phosphate backbone is an important microbial epigenetic modification governed by DndABCDE, which together with DndFGH, constitutes a restriction-modification system. We show that up to 45% of 1,349 identified bacterial <I>dnd</I> systems exhibit the form of solitary <I>dndABCDE</I> without the restriction counterparts of <I>dndFGH</I>. The combination of epigenomics, transcriptome analysis, and metabolomics suggests that in addition to providing a genetic barrier against invasive DNA, PT modification is a versatile player involved in the epigenetic control of gene expression and the maintenance of cellular redox homeostasis. This finding provides evolutionary and functional insights into this unusual epigenetic modification. Our results imply that PT systems might evolve similar to other epigenetic modification systems with multiple cellular functions.</P><P>The chemical diversity of physiological DNA modifications has expanded with the identification of phosphorothioate (PT) modification in which the nonbridging oxygen in the sugar-phosphate backbone of DNA is replaced by sulfur. Together with DndFGH as cognate restriction enzymes, DNA PT modification, which is catalyzed by the DndABCDE proteins, functions as a bacterial restriction-modification (R-M) system that protects cells against invading foreign DNA. However, the occurrence of <I>dnd</I> systems across a large number of bacterial genomes and their functions other than R-M are poorly understood. Here, a genomic survey revealed the prevalence of bacterial <I>dnd</I> systems: 1,349 bacterial <I>dnd</I> systems were observed to occur sporadically across diverse phylogenetic groups, and nearly half of these occur in the form of a solitary <I>dndBCDE</I> gene cluster that lacks the <I>dndFGH</I> restriction counterparts. A phylogenetic analysis of 734 complete PT R-M pairs revealed the coevolution of M and R components, despite the observation that several PT R-M pairs appeared to be assembled from M and R parts acquired from distantly related organisms. Concurrent epigenomic analysis, transcriptome analysis, and metabolome characterization showed that a solitary PT modification contributed to the overall cellular redox state, the loss of which perturbed the cellular redox balance and induced <I>Pseudomonas fluorescens</I> to reconfigure its metabolism to fend off oxidative stress. An in vitro transcriptional assay revealed altered transcriptional efficiency in the presence of PT DNA modification, implicating its function in epigenetic regulation. These data suggest the versatility of PT in addition to its involvement in R-M protection.</P>
한국 영상매체 접촉과 관광지 이미지, 방문의도 간의 관계
왕명명(Ming Ming Wang),류시영(Si Young Ryu),송운강(Woon Gang Song) 한국관광연구학회 2011 관광연구저널 Vol.25 No.1
The purpose of this study is to explore the relationship between visual media`s contact and intention to visit after watching Korean dramas and movies. Korean Wave became one of the important research project in tourism field. From the literature review, the authors propose a new model of destination image formation process including the familiarity. In order to analyze the new model, data were collected from 335 students. This study carried out descriptive statistical analysis, reliability analysis, factor analysis and path analysis to test the model. The findings of this study are as follows: 1) the visual media contact frequency had positive influence on familiarity, 2) The familiarity had positive influence on cognitive image, affectional image and overall image, 3) The cognitive image had positive influence on affectional image and on overall image, and affectional image also had positive influence on overall image, 4) cognitive image, affectional image and the overall image showed the positive influence on potential tourists`s intention to visit. Based on the results of this study, several points were made from the perspectives of international tourism marketing. The limitations of the study and the future research directions are also discussed.
Ren, Haibo,Zhao, Wei,Wang, Liyou,Ryu, Si Ok,Gu, Cuiping Elsevier 2015 JOURNAL OF ALLOYS AND COMPOUNDS Vol.653 No.-
<P><B>Abstract</B></P> <P>Porous flower-like tin oxide (SnO<SUB>2</SUB>) structures were obtained using a hydrothermal method combined with a subsequent calcination and acid-washing process. The morphologies and crystal structures of the products were characterized by field emission scanning electron microscopy, transmission electron microscopy, energy dispersive spectroscopy, X-ray diffraction, X-ray photoelectron spectroscopy, and Brunauer–Emmett–Teller N<SUB>2</SUB> adsorption-desorption analyses. The process of inducing porosity begins with a flower-like nickel tin sulfur precursor. Thermal decomposition of this flower-like nickel tin sulfur precursor leads to an intimate mixture of porous flower-like NiO/SnO<SUB>2</SUB> hybrids. Porous SnO<SUB>2</SUB> flowers were obtained after removing the cubic phase NiO by an acid-washing process. Furthermore, the gas sensing properties of the as-prepared porous SnO<SUB>2</SUB> flowers to VOCs, such as ethanol, formaldehyde, benzene, toluene, and acetone, were investigated. The porous SnO<SUB>2</SUB> flowers showed a good response and reversibility to some organic vapors, such as ethanol and formaldehyde. The sensing responses to 100 ppm ethanol and formaldehyde were 42.4 and 24.8, respectively. The sensors also exhibited a good response to benzene, toluene, methanol, and 2-propanol. The relationship between the gas-sensing properties and the microstructure of the as-prepared flower-like SnO<SUB>2</SUB> structures was also examined.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Flower-like nickel tin sulfur structures were synthesized by hydrothermal method. </LI> <LI> Porous SnO<SUB>2</SUB> microflowers were obtained by treatment of the precursor. </LI> <LI> Porous SnO<SUB>2</SUB> microflowers exhibited excellent sensing properties to some gases. </LI> <LI> Excellent gas-sensing properties were attributed to the special 3D structures. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>