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A Novel Recombined Potato virus Y Isolate in China
Shuxin Han,Yanling Gao,Guoquan Fan,Wei Zhang,Cailing Qiu,Shu Zhang,Yanju Bai,Junhua Zhang,Carl Spetz 한국식물병리학회 2017 Plant Pathology Journal Vol.33 No.4
This study reports the findings of a distinct Potato virus Y (PVY) isolate found in Northeast China. One hundred and ten samples (leaves and tubers) were collected from potato plants showing mosaic symptoms around the city of Harbin in Heilongjiang province of China. The collected tubers were planted and let to grow in a greenhouse. New potato plants generated from these tubers showed similar symptoms, except for one plant. Subsequent serological analyses revealed PVY as the causing agent of the disease. A novel PVY isolate (referred to as HLJ-C-44 in this study) was isolated from this sample showing unique mild mosaic and crisped leaf margin symptoms. The complete genome of this isolate was analyzed and determined. The results showed that HLJ-C-44 is a typical PVY isolate. Phylogenetic analysis indicated that this isolate belongs to the N-Wi strain group of PVY recombinants (PVYN-Wi) and also shared the highest overall sequence identity (nucleotide and amino acid) with other members of this strain group. However, recombination analysis of isolate HLJ-C-44 revealed a recombination pattern that differed from that of other PVYN-Wi isolates. Moreover, biological assays in four different potato cultivars and in Nicotiana tabacum also revealed a different phenotypic response than that of a typical PVYN-Wi isolate. This data, combined, suggest that HLJ-C-44 is a novel PVY recombinant with distinct biological properties.
Making Line Undercut Structure by Incremental Laser Shock Forming
Shuxin You,Xiangying Wang,Zhong Ji,Chao Zheng,Guofang Zhang,Ren Liu 한국정밀공학회 2019 International Journal of Precision Engineering and Vol.20 No.8
A novel technology to plastically deform line undercut structures by pulsed laser, named as incremental laser shock forming (ILSF) of sheet metal, is presented in this paper and is investigated by experiments and numerical simulations. The deformation of a line undercut structure can be considered as the accumulation of several point undercuts, the shape evolution during ILSF of copper foils was observed, and the thickness distribution on a cross-section was measured. The influences of laser energy E, forming height H, and laser spot overlap rate η on the process were studied and a process window was established to avoid deformation failure. The results show that the plastic deformation at η = 0% is less uniform and more vulnerable than that at η = 50%. Three typical failure modes were concluded and discussed. Finite element method simulation was conducted to verify the feasibility of forming line undercut structure by ILSF, and to analyze its deformation mechanism.
Viscoelastic characterization of the mucus from the skin of loach
Shuxin Huang 한국유변학회 2021 Korea-Australia rheology journal Vol.33 No.1
The epidermal mucus secreted by fish is a natural barrier between fish and water, which is a slippery and slightly thick fluid with some biological functions. The present work is to characterize the viscoelastic property of a loach skin mucus published recently by using a viscoelastic model with four hypotheses. The experimental steady shear viscosity with the maximum shear rate of 10 s1 for the mucus was fitted to obtain the parameters in the model. The other four steady shear viscosity curves with the maximum shear rate of 0.004, 0.01, 0.1, and 1.0 s1, respectively, and the start-up experiment at the shear rate of 0.1 s1 were predicted. The phenomenon that the viscosity at the low shear rate in the decreasing shear rate regime is higher than that in the increasing shear rate regime can be illuminated by a structural irreversibility hypothesis, which is based on the varying linear viscoelastic property of the mucus induced by shear rate. The theoretical description on the unusual viscosity curves of the loach mucus provides a base for quantitative analysis on the mucus effect on the swimming of fish, and could promote the understanding on the swimmingrelated function of skin mucus in fish.