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안지선(Ji-Seon An),오한진(Han-Jin Oh),윤원(Won Yun),이지환(Ji-Hwan Lee),곽우기(Woo-Gi Kwak),이창희(Chang-Hee Lee),류수동(Shu-Dong Liu),조진호(JinHo Cho) 충북대학교 동물생명과학연구소 2018 동물생명과학연구 Vol.10 No.-
The objective of this study was to investigate the effects of Essential oils (EO) on the storage and improvement of quality of dog food. These treatments were as follows : CON (ether extract (EE)/ crude protein (CP) ratio 〈 0.5), and TRT (EE/CP ratio > 0.5). In the test for antioxidant effect, EO was sprayed to the food surface to observe the delay of corruption by external observation. In the test for quality improvement, the sample was diluted and the microorganism was observed. With regard to decayed time, storage time was improved in EO treatment than CON on 1.1 days. Lactobacillus content was significantly increased (P 〈 0.05) in EO treatment than CON. Salmonella and E. coli contents were significantly decreased (P 〈 0.05) in EO treatment than CON. In conclusion, antioxidant function and storage stability of the feeds with the EE/CP ratio above 0.5 were improved by EO supplementation on dog diets. The quality of the feed was improved by controling contents of pathogen microorganisms.
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EMBRYOS OF EARLY CRETACEOUS CHORISTODERA (REPTILIA) FROM THE JEHOL BIOTA IN WESTERN LIAONING, CHINA
Qiang Ji,Shu-an Ji,Junchang Lu,Hailu You,Chong-xi Yuan 한국고생물학회 2006 고생물학회지 Vol.22 No.1
Choristodera는 잘 알려지지 않은 그룹이지만 매우 독특한 해양 파충류로 후기 트라이아스기에서부터 후기 올리고세까지 아시아, 북미, 유럽에 걸쳐 산출된다. 비록 수천 개의 choristodera 표본이 중국 전기 백악기 Jehol biota에서 수집되었지만 바다거북처럼 난태생이였는지 Pachypleurosauria (해양파충류) Keichousaurus hui처럼 태생이었는지 아직 확실하게 밝혀지지 않았다. 우리는 이 논문에서 중국 서부 요녕성의 Jiufotang층에서 산출된 가죽질의 알껍데기가 함께 잘 보존된 Hyphalosaurus baitaigouensis (파충류, Choristodera)를 보고한다. 이것은 Hyphalosaurus baitai-gouensis 같은 choristoderian 파충류가 남중국 Guizhou에서 산출된 후기 트라이아스기 Keichousaurus hui처럼 태생이었을 것임을 지시하는 직접적인 첫 번째 증거다. The Choristodera is a poorly known clade, but very a distinctive group of aquatic reptiles, which has been found from the Late Triassic to the Late Oligocene in Asia, North America and Europe. Although thousands of choristoderian specimens have been collected from the Early Cretaceous non-marine beds in Liaoning, China, no direct evidences have been found to determine whether they were oviparous like sea turtles or viviparous like Keichousaurus hui of Pacbypleurosauria (marine reptiles). Here we report a well-preserved specimen of Hyphalosaurus baitaigouensis with leathery embryonic eggs from the Jiufotang Formation in western Liaoning, China. It provides the first direct evidence to indicate that choristoderian reptiles are most likely viviparous like Late Triassic Keichousaurus hui from Guizhou, Southern China.
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Chao Du,Ju-Hua Ni,Ya-Qiong Jin,Jun-Juan Qi,Zhen-Xing Ji,Shu-Yan Li,Guo-Shun An,Hong-Ti Jia 한국분자세포생물학회 2012 Molecules and cells Vol.34 No.2
MyoD and myogenin (Myog) recognize sets of distinct but overlapping target genes and play different roles in skeletal muscle differentiation. MyoD is sufficient for near-full expression of early targets, while Myog can only partially enhance expression of MyoD-initiated late muscle genes. However, the way in which Myog enhances the expression of MyoD-initiated late muscle genes remains unclear. Here, we examine the effects of Myog on chromatin remodeling at late muscle gene promoters and their activation within chromatin environment. Chromatin immunoprecipitation (ChIP) assay showed that Myog selectively bound to the regulatory sequences of late muscle genes. Overexpres-sion of Myog was found to overcome sodium butyrate-inhibited chromatin at late muscle genes in differ-entiating C2C12 myoblasts, shifting the transcriptional activation of these genes to an earlier time period. Furthermore, overexpression of Myog led to increased hyperacetylation of core histone H4 in differentiating C2C12 myoblasts but not NIH3T3 fibroblasts, and hyperacetylated H4 was associated directly with the late muscle genes in differentiating C2C12, indicating that Myog can induce chromatin remodeling in the presence of MyoD. In addition, co-immunopre-cipitation (CoIP) revealed that Myog was associated with the nuclear protein Brd4 in differentiating C2C12 myoblasts. Together, these results suggest that Myog enhances the expression of MyoD-initiated late muscle genes through MyoD-dependent ability of Myog to induce chromatin remodeling, in which Myog-Brd4 interaction may be involved.
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