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Shou-Lu Lee 한국사회복지학회 2017 Asian Social Work and Policy Review Vol.11 No.2
In the past two decades, intra-Asia cross-border marriage immigration has become a significant phenomenon. This study collected questionnaire data from Bachelor of Social Work students (n = 1,232) in Taiwan to explore the influences of multicultural education, intergroup contact experience, and demographic characteristics on students’ attitudes toward female Southeast Asian marriage migrants. Regression analysis revealed that students’ attitudes are improved by direct contact with female Southeast Asian marriage migrants but are not correlated with students’ demographic characteristics. Although pre-college multicultural education improves students’ attitudes, college-level multicultural education adversely affects the attitudes of students who perform poorly in multicultural courses. These findings have valuable implications for social work educators.
Shou-Lu Lee 한국사회복지학회 2014 Asian Social Work and Policy Review Vol.8 No.3
This study is the first to explore the effect of social worker manpower on family violence incidents. In addition, it is the first study to investigate the geography of family violence in Taiwan. Using data from the County-Level Important Indicator Query System, this study performs panel regressions to analyze the determinants of county-level family violence incidents between 2005 and 2011. The empirical results clearly indicate that family violence incidents tend to be higher in counties that suffer economic disadvantage and in which there is an inadequate deployment of social worker manpower. The findings have important implications for combating family violence.
( Ming Shou Lu ),( Soo Jin Lee ),( Bo Rim Kim ),( Chang Hun Park ),( Min Kyu Oh ),( Kyung Moon Park ),( Sang Yup Lee ),( Jin Won Lee ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.5
2,3-Butanediol (2,3-BDO) is an organic compound with a wide range of industrial applications. Although Escherichia coli is often used for the production of organic compounds, the wild-type E. coli does not contain two essential genes in the 2,3-BDO biosynthesis pathway, and cannot ferment 2,3-BDO. Therefore, a 2,3-BDO biosynthesis mutant strain of Escherichia coli was constructed and cultured. To determine the optimum culture factors for 2,3-BDO production, experiments were conducted under different culture environments ranging from strongly acidic to neutral pH. The extracellular metabolite profiles were obtained using high-performance liquid chromatography (HPLC), and the intracellular metabolite profiles were analyzed by ultra-performance liquid chromatography and quadruple time-of-flight mass spectrometry (UPLC/ Q-TOF-MS). Metabolic flux analysis (MFA) was used to integrate these profiles. The metabolite profiles showed that 2,3-BDO production favors an acidic environment (pH 5), whereas cell mass favors a neutral environment. Furthermore, when the pH of the culture fell below 5, both the cell growth and 2,3-BDO production were inhibited.
Dynamic Modeling of Lactic Acid Fermentation Metabolism with Lactococcus Lactis
Euh Lim Oh,Ming Shou Lu,Chang Hun Park,Han Bin Oh,Sang Yup Lee,Jin Won Lee 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.2
A dynamic model of lactic acid fermentation using Lactococcus lactis was constructed, and a metabolic flux analysis (MFA) and metabolic control analysis (MCA) were performed to reveal an intensive metabolic understanding of lactic acid bacteria (LAB). The parameter estimation was conducted with COPASI software to construct a more accurate metabolic model. The experimental data used in the parameter estimation were obtained from an LC-MS/MS analysis and time-course simulation study. The MFA results were a reasonable explanation of the experimental data. Through the parameter estimation, the metabolic system of lactic acid bacteria can be thoroughly understood through comparisons with the original parameters. The coefficients derived from the MCA indicated that the reaction rate of L-lactate dehydrogenase was activated by fructose 1,6-bisphosphate and pyruvate, and pyruvate appeared to be a stronger activator of L-lactate dehydrogenase than fructose 1,6-bisphosphate. Additionally, pyruvate acted as an inhibitor to pyruvate kinase and the phosphotransferase system. Glucose 6-phosphate and phosphoenolpyruvate showed activation effects on pyruvate kinase. Hexose transporter was the strongest effector on the flux through L-lactate dehydrogenase. The concentration control coefficient (CCC) showed similar results to the flux control coefficient (FCC).
( Kim Bo Rim ),( Soo Jin Lee ),( Joo Hong Park ),( Ming Shou Lu ),( Min Kyu Oh ),( Young Rok Kim ),( Jin Won Lee ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.9
2, 3-Butanediol (2, 3-BD) is a major metabolite produced by Klebsiella pneumoniae KCTC2242, which is a important chemical with wide applications. Three genes important for 2, 3-BD biosynthesis acetolactate decarboxylase (budA), acetolactate synthase (budB), and alcohol dehydrogenase (budC) were identified in K. pneumoniae genomic DNA. With the goal of enhancing 2, 3-BD production, these genes were cloned into pUC18K expression vectors containing the lacZ promoter and the kanamycin resistance gene to generate plasmids pSB1-7. The plasmids were then introduced into K. pneumoniae using electroporation. All strains were incubated in flask experiments and 2, 3-BD production was increased by 60% in recombinant bacteria harboring pSB04 (budA and budB genes), compared with the parental strain K. pneumoniae KCTC2242. The maximum 2, 3-BD production level achieved through fedbatch fermentation with K. pneumoniae SGJSB04 was 101.53 g/l over 40 h with a productivity of 2.54 g/l.h. These results suggest that overexpression of 2, 3-BD synthesisrelated genes can enhance 2,3-BD production in K. pneumoniae by fermentation.