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Shim, Yoon Bo,Park, Jang Su,Kang, Shin Won,Kim, Andre,Jeong, In Chul 한국생화학회 1978 Vol. No.
The interaction of cytochrome c with binary phospholipid mixtxres was investigated by solid-state ²H- and ^(31)P-NMR. To examine the effect of the interaction on the glycerol backbones, the glycerol moieties of phosphaddylcholine (PC), phosphafidylethanolamine (PE), phosphatidylglycerol (PG), and cardiolipin (CL) were specifically deuterated. On the binding of cytochrome c to the binary mixed bilayers, no changes in the quadrupole splittings of each of the components were observed for the PGPG, PE/CL and PFJ PG 6posomes. In contrast, the splittings of CL decreased on binding of protein to the PGCL liposomes, although those of PC did not change at all. This showed that cytochrome c specifically interacts with CL in PGCL bilayers, and penetrates into the lipid bilayer to some extent so as to perturb the dynamic structure of the glycerol backbone. This is distinctly different from the mode of interaction of cytochrome c with other binary mixed bilayers. In the ^(31)P-NMR spectra, line broadening and a decrease of the chemical shift anisotropy were observed on the binding of cytochrome c for all binary mixed bilayers that were examined. These changes were more significant for the PG CL bilayers. Furthermore, the line broadening is more significant for PC than for CL in PGCL bilayers. Therefore, it can be concluded that with the polar head groups, not only CL but also PC are involved in the interaction with cytochrome c.
FSR1 is essential for virulence and female fertility in Fusarium verticillioides and F. graminearum.
Shim, Won-Bo,Sagaram, Uma Shankar,Choi, Yoon-E,So, Jinny,Wilkinson, Heather H,Lee, Yin-Won APS Press 2006 Molecular plant-microbe interactions Vol.19 No.7
<P>Fusarium verticillioides (teleomorph Gibberella moniliformis) and F. graminearum (teleomorph G. zeae) are well known to cause devastating diseases on cereal crops. Despite their importance, our understanding of the molecular mechanisms involved in these host-pathogen interactions is limited. The FSR1 locus in F. verticillioides was identified by screening REMI mutants for loss of virulence in maize stalk rot inoculation studies. FSR1 encodes an 823-codon open reading frame interrupted by two introns. The Fsr1 protein shares 60% sequence identity with the Sordaria macrospora Pro11, a multimodular protein with four putative protein-protein binding domains (caveolin-binding domain, coiled-coil structure, calmodulin-binding motif, and seven-WD40 repeats), which plays a regulatory role in cell differentiation and ascocarp development. Our data demonstrate that FSR1 is essential for female fertility and virulence in F. verticillioides. Significantly, targeted disruption of the FSR1 ortholog in F. graminearum (FgFSR1) reduced virulence on barley and deterred perithecia formation. Cross-complementation experiments demonstrated that the gene function is conserved in the two Fusarium species. FSR1 is expressed constitutively, and we hypothesize that Fsr1 regulates virulence by acting as a scaffold for a signal transduction pathway. A survey of available genome databases indicates Fsr1 homologs are present in a number of filamentous fungi and animal systems but not in budding yeast or plants. A maximum likelihood analysis of this gene family reveals well-supported monophyletic clades associated with fungi and animals.</P>
Shim, Jang Bo,Lee, Suk,Cho, Sam Ju,Lee, Sang Hoon,Kim, Juree,Cho, Kwang Hwan,Min, Chul Kee,Huh, Hyun Do,Lee, Rena,Yang, Dae Sik,Park, Young Je,Yoon, Won Seob,Kim, Chul Yong,Kwon, Soo Il science press 2010 Chinese physics. C Vol.34 No.11
<P>This study compares and analyzes stereotactic radiotherapy using tomotherapy and linac-based fractionated stereotactic radiotherapy in the treatment of intra-cranial tumors, according to some cases. In this study, linac-based fractionated stereotactic radiotherapy and tomotherapy treatment were administered to five patients diagnosed with intra-cranial cancer in which the dose of 18–20 Gy was applied on 3–5 separate occasions. The tumor dosing was decided by evaluating the inhomogeneous index (II) and conformity index (CI). Also, the radiation-sensitive tissue was evaluated using low dose factors <I>V</I><SUB>1</SUB>, <I>V</I><SUB>2</SUB>, <I>V</I><SUB>3</SUB>, <I>V</I><SUB>4</SUB>, <I>V</I><SUB>5</SUB>, and <I>V</I><SUB>10</SUB>, as well as the non-irradiation ratio volume (NIV). The values of the II for each prescription dose in the linac-based non-coplanar radiotherapy plan and tomotherapy treatment plan were (0.125±0.113) and (0.090±0.180), respectively, and the values of the CI were (0.899±0.149) and (0.917±0.114), respectively. The low dose areas, <I>V</I><SUB>1</SUB>, <I>V</I><SUB>2</SUB>, <I>V</I><SUB>3</SUB>, <I>V</I><SUB>4</SUB>, <I>V</I><SUB>5</SUB>, and <I>V</I><SUB>10</SUB>, in radiation-sensitive tissues in the linac-based non-coplanar radiotherapy plan fell into the ranges 0.3%−95.6%, 0.1%−87.6%, 0.1%−78.8%, 38.8%-69.9%, 26.6%-65.2%, and 4.2%−39.7%, respectively, and the tomotherapy treatment plan had ranges of 13.6%−100%, 3.5%−100%, 0.4%−94.9%, 0.2%−82.2%, 0.1%−78.5%, and 0.3%−46.3%, respectively. Regarding the NIV for each organ, it is possible to obtain similar values except for the irradiation area of the brain stem. The percentages of NIV 10%, NIV20%, and NIV30%for the brain stem in each patient were 15%−99.8%, 33.4%−100%, and 39.8%−100%, respectively, in the fractionated stereotactic treatment plan and 44.2%-96.5%, 77.7%-99.8%, and 87.8%−100%, respectively, in the tomotherapy treatment plan. In order to achieve higher-quality treatment of intra-cranial tumors, treatment plans should be tailored according to the isodose target volume, inhomogeneous index, conformity index, position of the tumor upon fractionated stereotactic radiosurgery, and radiation dosage for radiation-sensitive tissues.</P>
( Bo Young Lee ),( Jae Young Jang ),( Soung Won Jeong ),( Gene Hyun Bok ),( Jeong Ho Ham ),( Joo Young Cho ),( Joon Seong Lee ),( Chan Sup Shim ) 대한소화기기능성질환·운동학회(구 대한소화관운동학회) 2011 Gut and Liver Vol.5 No.2
We report two cases of adrenal abscesses that occurred following a Histoacryl(R) (N-butyl-2-cyanocrylate) injection for variceal bleeding. Patients had been diagnosed with alcoholic liver cirrhosis and gastric varices bleeding and received a Histoacryl(R) injection for the variceal bleeding. Patients had fever and abdominal tenderness and were diagnosed with an adrenal abscess at 2 months following the Histoacryl(R) injection. One patient received open drainage and the other underwent percutaneous drainage. When a patient has previously been injected with Histoacryl(R) for the treatment of variceal bleeding and presents with fever, an evaluation for an unusual complication such as adrenal abscess is recommended. (Gut Liver 2011;5:242-244)
Anti-inflammatory and radical scavenging properties of Verbena officinalis
Shim, Hwan-Ki,Kim, Seong-Yeol,Kim, Bo-Rim,Cho, Jae-Park,Park, Yae-Jeong,Ji, Won-Geun,Cha, Dong-Seok,Jeon, Hoon Kyung Hee Oriental Medicine Research Center 2010 Oriental pharmacy and experimental medicine Vol.10 No.4
Verbena officinalis (Verbenaceae) has been used as herbal medicine or health supplement in both Western and Eastern countries for centuries. In the present study, we examined the anti-inflammatory and antioxidant activities of the methylene chloride fraction of V. officinalis (VMC). To elucidate the anti-inflammatory properties of VMC, we investigated the inhibition effects of nitric oxide production in interferon-gamma (IFN-$\gamma$) and lipopolysaccharide-stimulated mouse peritoneal macrophages. VMC suppressed nitric oxide production, inducible nitric oxide synthase and cyclooxygenase-2 expression dose-dependently without notable cytotoxicity. In various radical scavenging assays, VMC exhibited strong scavenging effect on DPPH radical, superoxide radical, nitric oxide radical and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) radical. VMC also showed potent reducing power. These findings strongly suggest that VMC may be beneficial in oxidative stress-mediated inflammatory disorders.
Shim, Yoon Bo,Won, Mi Sook,Jeong, Gi ho 부산대학교 1981 자연과학논문집 Vol.32 No.-
Copper, lead and cadmium in natural water determined by anodic stripping voltammetry with solvent extraction. A glassy carbon electrode was also used. 50㎖ of sample solution was extracted three times with 10㎖ of 0.01% dithizone-chloroform. When the chloroform layer was back extracted with 10㎖ 0.1 N HCl containing excess mercuric ions, the free metal ions come into aqueous layer. 5㎖ of this aqueous solution contained residual mercuric ion was not stripped into chloroform layer but copper, lead and cadmium ions were back extracted in aqueous layer. And then the anodic stripping curve was recorded. The concentration of copper, lead can be determined up to 4 ppb and that of cadmium up to 6ppb within an error of 10%.