Comparative Evaluation of Hepatoprotective Activities of Geniposide, Crocins and Crocetin by CCl4-Induced liver Injury in Mice

( Ping Chen ),( Yang Chen ),( Ya Rong Wang ),( Shi Ning Cai ),( Liang Deng ),( Jia Liu ),( Hao Zhang ) 한국응용약물학회 2016 Biomolecules & Therapeutics(구 응용약물학회지) Vol.24 No.2

Iridoid glycosides (mainly geniposide) and crocetin derivatives (crocins) are the two major active constituents in Gardenia jasminoides Ellis. In the present study, geniposide, crocins, crocin-1 and crocetin were separated from gardenia chromatographically. Then, mice were orally administrated with geniposide (400 mg/kg b.w.), crocins (400 mg/kg b.w.), crocin-1 (400 mg/kg b.w.) and crocetin (140 mg/kg b.w.) once daily for 7 days with CCl4. Hepatoprotective properties were evaluated by biochemical parameters: Administration of geniposide, crocins, crocin-1and crocetin significantly lowered serum alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP) levels in CCl4-treated mice. The reduced glutathione (GSH) levels and antioxidant enzymes (SOD and CAT) activities were also increased by geniposide, crocins, crocin-1 and crocetin. Histopathological examination of livers showed that these components reduced deformability, irregular arrangement and rupture of hepatocyte in CCl4-treated mice. These biochemical results and liver histopathological assessment demonstrated that geniposide, crocetin derivatives and crocetin show comparative beneficial effects on CCl4-induced liver damage via induction of antioxidant defense. Therefore, contents of geniposide and crocetin derivatives should be both considered for hepatoprotective efficacy of Gardenia jasminoides Ellis.

Research on Automatic Compensation Technology for Eccentricity of Grinding Wheel

Shi-Ping Chen,Zhenzhong Wang,Hui Yu,Long-Qiao Lin 한국정밀공학회 2018 International Journal of Precision Engineering and Vol.19 No.8

To ensure the installation precision of grinding wheel in precision grinding, the attitude of spindle axis must be adjusted to coincide with the geometric center of grinding wheel before the process. A special device that combined with piezoelectric actuators with threedegrees- of-freedom is designed. The corresponding method of automatic compensation technology for eccentricity of grinding wheel is developed. In order to calculate accurately eccentric angle and the eccentricity of the grinding wheel, high accuracy detection algorithm for eccentric signal of grinding wheel is given by the principle of rotary roundness error minimization firstly. Then a decomposition algorithm is proposed to decompose the eccentricity into three directions and the eccentricity can be eliminated by the coordinated control of the three piezoelectric actuators. Experimental results show that the average accuracy of the system is about 5.48 μm. Compared with the sine fitting algorithm, the algorithm presented for adjusting the eccentricity of grinding wheel in this paper is more stable and the accuracy is improved by about 78.2%, which shows that the proposed approach works successfully.

QTLDissection of Agronomic and Domestication Traits Using Introgression Lines Carrying Wild Rice (Oryza rufipogon Griff.) Segments in Cultivated Rice (O. sativa L.) Background

Ping-Rong Yuan,Hyun-Jung Kim,Qiong-Hua Chen,Hong-Guang Ju,Seung-Joon Lee,Shi-Dong Ji,안상낙 한국작물학회 2009 Journal of crop science and biotechnology Vol.12 No.4

The nearly isogenic nature of the introgression lines (ILs) provides a relative advantage over other segregating populations in therapid implementation of pyramiding approach through crosses and marker analysis. A set of 126 ILs carrying various introgressedsegments from a presumed wild progenitor, Oryza rufipogon Griff. Acc. W1944 in the background of an elite Korea japonica cultivar(O. sativa L.), Hwayeongbyeo, was constructed using the marker assisted selection (MAS) technique combined with repeatedbackcrosses. The 126 ILs have different W1944 segments on each chromosome, with 100% coverage of wild segments on chromosome1, while for chromosome 10, the coverage was only 33.3%. The mean number of homozygous and heterozygous donor segmentswere 3 (ranging from 0 to 7) and 4.7 (ranging from 1 to 14.5), respectively, and 31.6% of introgressed segments had sizes ofless than 10.5 cM. A total of 41 quantitative trait loci (QTLs) and two loci associated with pericarp coloration were identified. Thenumber of QTLs per trait ranged from 2 to 6. Phenotypic variance associated with each QTL varied from 9.1 to 52.2%, with an averageof 17.1%. For 11 (26.8%) of the QTLs detected in this study, the O. rufipogon-derived alleles contributed the desired agronomiceffect despite the overall inferior characteristics of the wild phenotype. Favorable alleles from the O. rufipogon accession were identifiedfor panicle number, panicle length, days to heading, secondary branches, spikelets per panicle, and 1,000-grain weight.

A Generalized Markov Chain Model for IEEE 802.11 Distributed Coordination Function

( Ping Zhong ),( Jianghong Shi ),( Yuxiang Zhuang ),( Huihuang Chen ),( Xuemin Hong ) 한국인터넷정보학회 2012 KSII Transactions on Internet and Information Syst Vol.6 No.2

To improve the accuracy and enhance the applicability of existing models, this paper proposes a generalized Markov chain model for IEEE 802.11 Distributed Coordination Function (DCF) under the widely adopted assumption of ideal transmission channel. The IEEE 802.11 DCF is modeled by a two dimensional Markov chain, which takes into account unsaturated traffic, backoff freezing, retry limits, the difference between maximum retransmission count and maximum backoff exponent, and limited buffer size based on the M/G/1/K queuing model. We show that existing models can be treated as special cases of the proposed generalized model. Furthermore, simulation results validate the accuracy of the proposed model.

Mapping QTLs for Grain Quality Using an Introgression Line Population from a Cross between Oryza sativa and O. rufipogon

Ping-Rong Yuan,Shi-Dong Ji,안상낙,김현정,Qiong-Hua Chen,Hong-Guang Ju 한국작물학회 2010 Journal of crop science and biotechnology Vol.13 No.4

Quantitative trait loci (QTL) associated with four milling recovery properties, two chemical properties, six paste properties of grain, and six textural parameters of cooked rice were identified using an introgression line (IL) population of rice developed from an interspecific cross over two years. The IL population consisted of 121 lines from a cross between wild rice (O. rufipogon Griff.) and a japonica cultivar. A total of 28 QTLs were identified for the 14 traits using inter mapping. Of these, 10 were significant over two years indicating that these QTLs are stable across years and environments. For eight (21%) of the QTLs identified, the O. rufipogonderived alleles contributed a desirable effect on amylose content, protein content, minimum viscosity, final viscosity, and consistency. Among these, pc3 for protein content and ac7 for amylose content were significant in both years and showed an R² value of 25.5and 30.9%, respectively. The markers closely associated with these useful alleles can be used to trace the inheritance of specific chromosome segments in the IL population and also offer a starting point for map-based cloning of genes underlying these traits.

Investigation of Porous Structure of Aerogel Prepared from Nanofibrillated Cellulose

Chen Gong,Yu Shi,Jian-Ping Ni,Xiao-Bo Yang,Yan-Zhao Liu,Chao Tian 한국펄프·종이공학회 2016 펄프.종이기술 Vol.48 No.6

Cellulose aerogels were prepared through changing the concentration of TEMPO-oxidized cellulose suspension and applying high-pressure homogenization, followed by regular or rapid freeze drying methods. The prepared cellulose aerogel was studied by scanning electron microscopy, BET analysis (nitrogen gas adsorption), and mercury intrusion porosimetry to investigate the effect of variables on the porous properties. In the present study, the porous structure of cellulose aerogel was well obtained by varying concentration of NFC and applying rapid freeze drying approach. The specific surface area of prepared cellulose aerogels varied linearly with adjusting concentrations of NFC, and the highest surface area of cellulose aerogel was attained at 2.0% concentration of NFC in this study. Compared to regular freezing in the freezer, rapid freezing in the liquid nitrogen prepared cellulose aerogel has a lower BET value but more uniform structure with relatively narrow pore size distribution.

Endophytic Trichoderma gamsii YIM PH30019: a promising biocontrol agent with hyperosmolar, mycoparasitism, and antagonistic activities of induced volatile organic compounds on root-rot pathogenic fungi of Panax notoginseng

Chen, Jin-Lian,Sun, Shi-Zhong,Miao, Cui-Ping,Wu, Kai,Chen, You-Wei,Xu, Li-Hua,Guan, Hui-Lin,Zhao, Li-Xing The Korean Society of Ginseng 2016 Journal of Ginseng Research Vol.40 No.4

Background: Biocontrol agents are regarded as promising and environmental friendly approaches as agrochemicals for phytodiseases that cause serious environmental and health problems. Trichoderma species have been widely used in suppression of soil-borne pathogens. In this study, an endophytic fungus, Trichoderma gamsii YIM PH30019, from healthy Panax notoginseng root was investigated for its biocontrol potential. Methods: In vitro detached healthy roots, and pot and field experiments were used to investigate the pathogenicity and biocontrol efficacy of T. gamsii YIM PH30019 to the host plant. The antagonistic mechanisms against test phytopathogens were analyzed using dual culture, scanning electron microscopy, and volatile organic compounds (VOCs). Tolerance to chemical fertilizers was also tested in a series of concentrations. Results: The results indicated that T. gamsii YIM PH30019 was nonpathogenic to the host, presented appreciable biocontrol efficacy, and could tolerate chemical fertilizer concentrations of up to 20%. T. gamsii YIM PH30019 displayed antagonistic activities against the pathogenic fungi of P. notoginseng via production of VOCs. On the basis of gas chromatography-mass spectrometry, VOCs were identified as dimethyl disulfide, dibenzofuran, methanethiol, ketones, etc., which are effective ingredients for antagonistic activity. T. gamsii YIM PH30019 was able to improve the seedlings' emergence and protect P. notoginseng plants from soil-borne disease in the continuous cropping field tests. Conclusion: The results suggest that the endophytic fungus T. gamsii YIM PH30019 may have a good potential as a biological control agent against notoginseng phytodiseases and can provide a clue to further illuminate the interactions between Trichoderma and phytopathogens.

Overexpression of RUNX3 Inhibits Malignant Behaviour of Eca109 Cells in Vitro and Vivo

Chen, Hua-Xia,Wang, Shuai,Wang, Zhou,Zhang, Zhi-Ping,Shi, Shan-Shan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.4

Runt-related transcription factor 3 (RUNX3) is a tumor suppressor gene whose reduced expression may play an important role in the development and progression of esophageal squamous cell cancer (ESCC). The aim of this study was to investigate the clinical relevance of RUNX3 in ESCC patients and effects of overexpression on biological behaviour of Eca109 cells in vitro and in vivo. Immunohistochemistry was performed to detect the clinical relevance of RUNX3 and lymph node metastasis in 80 ESCC tissues and 40 non-cancerous tissues using the SP method. RT-PCR and Western blotting were applied to assess the RUNX3 level and verify the Eca109 cell line with stable overexpression. Localization of RUNX3 proteins was performed by cell immunofluorescence. CCK-8 and Scrape motility assays were used to determine proliferation and migration and the TUNEL assay to analyze cell apoptosis. Invasive potential was assessed in cell transwell invasion experiments. In nude mice, tumorigenesis in vivo was determined. Results showed decreased expression of RUNX3 in esophageal tissue to be significantly related to lymph node metastasis (LNM) (P<0.01). In addition, construction of a recombinant lentiviral vector and transfection into the human ESCC cell line Eca109 demonstrated that overexpression could inhibit cell proliferation, migration and invasion, and induce apoptosis. The in vivo experiments in mice showed tumorigenicity and invasiveness to be significantly reduced. Taken together, our studies indicate that underexpression of RUNX3 in human ESCC tissue is significantly correlated with progression. Restoration of RUNX3 expression significantly inhibits ESCC cells proliferation, migration, invasion and tumorigenesis.

Facile Synthesis of Graphene–Enwrapped Ag3PO4 Composites with Highly Efficient Visible Light Photocatalytic Performance

Shi Lei,Da Chen,Wenting Xie,Jing Zhang,Guangxing Ping,Meiqiang Fan,Laishun Qin,Liqun Bai,Zhi Chen,Chunju Lv,Kangying Shu 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.1

In this work, thermally exfoliated graphene nanosheets (GNS) were employed to prepare novel Ag3PO4–GNS composite photocatalysts by a facile chemical precipitation approach. The asprepared Ag3PO4–GNS composite photocatalysts were characterized by X-ray diffraction (XRD) pattern, field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), Raman spectroscopy, thermogravimetric (TG) analysis, ultraviolet-visible diffuse re-flectance spectroscopy (DRS) and photoluminescence (PL) spectra. It was found that the Ag3PO4 particles were well deposited on the surfaces of GNS. Compared with bare Ag3PO4 and Ag3PO4–rGO composite, the Ag3PO4–GNS composite exhibited enhanced photocatalytic activity for the photodegradation of rhodamine B (RhB) under visible light irradiation. The photocatalytic degradation rate of Ag3PO4–GNS composite was 1.7 times that of bare Ag3PO4 and about 1.3 times that of Ag3PO4–rGO for the degradation of RhB. Furthermore, the photocatalytic stability of Ag3PO4–GNS composite was also greatly enhanced. This enhanced photocatalytic activity and stability could be ascribed to the positive synergetic effects between the Ag3PO4 particles and GNS sheets, which could provide a greater number of active adsorption sites, suppress charge recombination and reduce the serious photocorrosion of Ag3PO4. Moreover, the photocatalytic degradation of RhB over Ag3PO4–GNS composites was also optimized, suggesting that the optimal amount of GNS in the composites was 11.4 wt.%. This work shows a great potential of Ag3PO4–GNS composite for environmental treatment of organic pollutants.

Triterpenoids from Schisandra henryi with Cytotoxic Effect on Leukemia and Hela Cells In Vitro

Chen, Ye-Gao,Wu, Zheng-Cai,Lv, Yu-Ping,Gui, Shi-Hong,Wen, Jin,Liao, Xin-Rong,Yuan, Li-Ming,Halaweish, Fathi The Pharmaceutical Society of Korea 2003 Archives of Pharmacal Research Vol.26 No.11

Four known lanostane triterpenoids, schiprolactone A (1), schisanlactone B (2), nigranoic acid (3) and schisandronic acid (4) Were isolated from the stems of Schisandra henryi for the first time. Their structures were characterized by IR, MS and NMR techniques. Compounds 1, 2 and 4 showed moderate cytotoxic activity against Leukemia cells in vitro. Cytotoxic activity of compounds 1-4 showed $IC_{50}$ of 0.0097, 0.01, 0.097 and 0.0099 $\mu$ mol/mL respectively toward Leukemia cells and $IC_{50}$ of 0.097, 0.1, 0.097 and 0.099 $\mu$mol/mL toward Hela cells respectively. It is the first report that these compounds possess cytotoxic activity on Leukemia and Hela cells.