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RISS 인기검색어
- 검색키워드
- 저자 : Shaheen Amna Kashif (검색결과 4 건)
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Amna, Kashif Shaheen,Park, So Yeon,Choi, Min,Kim, Sang Yeon,Yoo, Ah Young,Park, Jae Kweon The Korean Society for Marine Biotechnology 2018 한국해양바이오학회지 Vol.10 No.1
The aim of this study is to elucidate the biochemical properties of manno-oligosaccharides (MOS) hydrolyzed by extracellular enzyme of Bacillus N3. We strived to characterize the biochemical properties of MOS since N3 can effectively hydrolyzed natural polymannans such as galactomannan (GM) and konjac (glucomannan, KM), respectively. The hydrolysis of GM and KM was applied by the strain N3 in terms of reducing sugars and the highest production of reducing sugars was estimated to be about 750 mg/L and 370 mg/L respectively, which were quantified after 7 days of cultivation in the presence of both substrates. Hydrolysates derived from the hydrolysis of KM showed the significant antioxidant activity based on DPPH and ABTS radical scavenging activity with increasing of tyrosinase inhibitory activity. On the other hand, hydrolysates derived from the hydrolysis of GM showed only ABTS radical scavenging activity without showing significant changes on tyrosinase inhibitory activity. Our data suggest that those biological characteristics may be depend on the primary structure and the size of MOS, which may be useful as potent additives for diet foods.
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Kashif Shaheen Amna,So Yeon Park,Min Choi,Sang Yeon Kim,Ah Young Yoo,Jae Kweon Park 한국해양바이오학회 2018 한국해양바이오학회지 Vol.10 No.1
The aim of this study is to elucidate the biochemical properties of manno-oligosaccharides (MOS) hydrolyzed by extracellular enzyme of Bacillus N3. We strived to characterize the biochemical properties of MOS since N3 can effectively hydrolyzed natural polymannans such as galactomannan (GM) and konjac (glucomannan, KM), respectively. The hydrolysis of GM and KM was applied by the strain N3 in terms of reducing sugars and the highest production of reducing sugars was estimated to be about 750 ㎎/L and 370 ㎎/L respectively, which were quantified after 7 days of cultivation in the presence of both substrates. Hydrolysates derived from the hydrolysis of KM showed the significant antioxidant activity based on DPPH and ABTS radical scavenging activity with increasing of tyrosinase inhibitory activity. On the other hand, hydrolysates derived from the hydrolysis of GM showed only ABTS radical scavenging activity without showing significant changes on tyrosinase inhibitory activity. Our data suggest that those biological characteristics may be depend on the primary structure and the size of MOS, which may be useful as potent additives for diet foods.
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( Shaheen Amna Kashif ),( Sang Hun Jung ),( Jae Kweon Park ) 한국키틴키토산학회 2020 한국키틴키토산학회지 Vol.25 No.4
Chitosan (CTSN) and β-glycerophosphate (GP) based hydrogels were prepared and characterized to elucidate the chemical interactions involved in the formation of these hydrogels depending upon the molecular weight of chitosan. Characterization of nanostructure of the resulting hydrogels was investigated by Fourier Transform-Infrared Spectroscopy (FT-IR). We observed that the major interaction which might be responsible for the formation of these hydrogels is between phosphate groups (PO<sub>4</sub> <sup>3-</sup> ) of GP and amine groups of chitosan. Whereas, the addition of hyaluronic acid (HA) which may act as a crosslinker has a negative effect on available free phosphate groups in the hydrogel confirmed by FT-IR analysis (971.9 cm <sup>-1</sup> peak). Decisively, the outcomes of spectrophotometric analysis also coincide with the findings of FT-IR for the CTSN/GP/HA thermo-hydrogel system. This study demonstrates the standard protocol to provide a practical approach for the analysis of interactions involved in the formation of CTSN/GP/HA hydrogels that could be potently useful in pharmacological and pharmaceutical applications.
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Enzymatically Hydrolyzed Water-Soluble Chitosan as a Potent Anti-Microbial Agent
Shaheen Amna Kashif,박제권 한국고분자학회 2019 Macromolecular Research Vol.27 No.6
Biological activities of chitosan depend upon its degree of polymerization (DP), fraction of acetylation (FA), and possibly on their pattern of acetylation (PA). Active molecular weight chitosan (AMC) was obtained from water soluble chitosan (WSC) by enzymatic hydrolysis over 600 h of time period. By the end of hydrolysis WSC was hydrolyzed to AMC with final concentration of 14 mM of reducing sugars with lowest viscosity (1.99 mm2/sec). Matrix assisted laser desorption/ionizationtime of flight mass spectrometer (MALDI-TOF) analysis has shown homo- and hetero- oligomers in the reaction mixture. Intense infra-red (IR) bands appeared through 500-3700cm-1 were assigned to be asymmetric and symmetric stretching of common sugars. WSC-AMC has inhibited 80% growth of Candida albicans and Penicillium italicum with highest zone of inhibition of 22 and 25 mm respectively. Mechanism of action for cellular growth inhibition involves the reactive oxygen species (ROS) elimination beyond the normal levels along with inactivation of β-lactamase like enzymes. Our results demonstrated that after hydrolysis the biological activities of WSC improved along with its flow properties and other biological activities.
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