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- 저자 : Seyed Siamak Ashraf Talesh (검색결과 2 건)
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Maryam Gazor,Seyed Siamak Ashraf Talesh,Alireza kavianpour,Maryam Khatami,Amin Javidanbardan,Seyed Nezamedin Hosseini 한국생물공학회 2018 Biotechnology and Bioprocess Engineering Vol.23 No.1
In biotechnological processes, often cell disruption has been an inevitable step as current host cells express most of the desired products intracellularly. Thus, an appropriate cell disruption technique must be selected considering different factors including the target product, process scale, and cell wall structure. In the current study, as a novel method, the efficacy of cell disruption via laser was tested qualitatively and quantitatively in batch and continuous systems, respectively. Laser-induced cell lysis can be a clean, rapid and convenient alternative to the other conventional disruption techniques. Our investigations in the continuous system with a flow rate of 800 μL/sec proved efficient (~ 90%) Pichia pastoris cell disruption at the wavenumber 1,064 nm with the energy input of 284 mW after four complete rounds of circulation. The main mechanism of cell disruption is assumed to be thermolysis via instant heat increase in the laser-treated spot. The results of the current study showed that continuous laser system could be applied in laboratory and industry scale for cell disruption.
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Reza Mahdavi,Seyed Siamak Ashraf Talesh 한국화학공학회 2021 Korean Journal of Chemical Engineering Vol.38 No.6
The selective sono/photocatalytic degradation of methyl orange (MO) in a mixture of methylene blue (MB) by synthesized ZnO/Al2O3 nanocomposites via ultrasound-assisted sol-gel method was studied. The synthesized samples were analyzed by XRD, TEM, EDX, BET, FTIR, UV-vis and Zeta potential analysis. The results showed that the ZnO nanoparticles were hexagonal wurtzite structures that changed to a flake-like quadrilateral morphology by enhancing the Al2O3 dosage. To optimize the selective removal efficiency of MO, response surface methodology (RSM) based on the central composite design (CCD) was applied to study the influence of Al2O3 dosage, catalyst amount, dye concentration and reaction time. A reliability prediction based on a second-order model was achieved with a high coefficient of determination (R2) and adjusted R2 of 0.9914 and 0.9834 for the optimized removal process, respectively. The MO optimal conditions of selective removal efficiency were found at 6.39 wt% of Al2O3 dosage, 32.2mg catalyst amount, 7mg/L of dye concentration and reaction time of 150min to achieve 91.42% removal of MO dye. In the optimal conditions of selective removal, the advanced sono/photocatalytic technique was used to investigate the complete degradation of the adsorbed MO on the catalyst surface. The FTIR spectrum of the MO degradation shows complete removal of some bands compared to the initial control dye, which confirms the degradation of MO after ultrasonic (US) and ultraviolet (UV) irradiation. The use of ZnO/Al2O3 nanocomposites in sonocatalysis process leads to producing electron/hole pairs, whereby the production of reactive oxygen species, MO selected molecules were oxidized quickly.
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