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Seyed Nezamedin Hosseini,Masoud Zendehzaban,Shahram Sharifnia 한국화학공학회 2013 Korean Journal of Chemical Engineering Vol.30 No.3
Photocatalytic degradation of ammonia on supported TiO2 nanoparticles was investigated. The TiO2 nanoparticles used as photocatalyst were coated on light expanded clay aggregate granules (LECA), which is a porous and light weight support. Photocatalytic reaction activity of prepared catalyst was determined by ammonia degradation from water synthetically polluted with ammonia. Experiment results showed significantly high photocatalytic activity for the immobilized catalysts. The ammonia was removed more than 85% within 300 min of the process with optimum calcinations temperature 550 oC and pH 11. Kinetics of the photocatalytic reaction followed a pseudo-first order model. XRF, XRD and SEM analyses revealed a rather uniform coating of TiO2 on the support. By using floated TiO2/LECA as a photocatalyst in aqueous solution of NH3, the ammonia was photodegraded into N2 and H2 gases, while NO2− and NO3− were formed at very low concentrations.
Maryam Gazor,Seyed Siamak Ashraf Talesh,Alireza kavianpour,Maryam Khatami,Amin Javidanbardan,Seyed Nezamedin Hosseini 한국생물공학회 2018 Biotechnology and Bioprocess Engineering Vol.23 No.1
In biotechnological processes, often cell disruption has been an inevitable step as current host cells express most of the desired products intracellularly. Thus, an appropriate cell disruption technique must be selected considering different factors including the target product, process scale, and cell wall structure. In the current study, as a novel method, the efficacy of cell disruption via laser was tested qualitatively and quantitatively in batch and continuous systems, respectively. Laser-induced cell lysis can be a clean, rapid and convenient alternative to the other conventional disruption techniques. Our investigations in the continuous system with a flow rate of 800 μL/sec proved efficient (~ 90%) Pichia pastoris cell disruption at the wavenumber 1,064 nm with the energy input of 284 mW after four complete rounds of circulation. The main mechanism of cell disruption is assumed to be thermolysis via instant heat increase in the laser-treated spot. The results of the current study showed that continuous laser system could be applied in laboratory and industry scale for cell disruption.