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대한민국 자동차 번호판에서 블록 이진화 기법을 이용한 문자 인식 기법
장인태, 이슬기, 우효정, 안재형 충북대학교 컴퓨터정보통신연구소 2012 컴퓨터정보통신연구 Vol.20 No.1
In this paper, the image binarization method using standard specifications of the vehicle license plate to recognize characters on the plates is proposed. The global binarization method that had a threshold over entire image pixels cannot acuratelly binarize in case that local average brightness are varied to each plates characters regions. The proposed method uses the characters regions in the standard specifications of the korean vehicle license plates as binarization blocks. For installed cameras on national roads and parking areas, the computer simulation results showed average characters recognition rate about 5% higher than the otzu global binarization method.
표정인식을 위한 PCA와 템플릿 정합을 사용한 얼굴 요소 추출
우효정, 이슬기, 김동우, 류성필, 안재형 충북대학교 산업과학기술연구소 2013 산업과학기술연구 논문집 Vol.27 No.2
This paper proposed an extracting algorithm of human facial components for the recognition of facial expressions. First face image is acquired using the Haar-like feature mask from an input image. The face image is divided into two images. One is the upper image including eye and eyebrow. The other is the lower image including mouth and jaw. The extraction of facial components, such as eye and mouth, begins getting eye image and mouth image. Then eigenfaces are produced by the PCA training process with learning images. An eigeneye and an eigenmouth are produced from eigenfaces. Result eye image is obtained by template matching the upper image with the eigeneye, and the mouth image is obtained by template matching the lower image with the eigenmouth. The simulation results show that the proposed method has superior extraction ratio than previous method.
Bioconverted Orostachys japonicas Extracts Suppress Angiogenic Activity of Ms-1 Endothelial Cells
Lee, Seul Gi,Kim, Jin Soo,Lee, Han-Saem,Lim, Yu-Mi,So, Jai-Hyun,Hahn, Dongyup,Ha, Yu Shin,Nam, Ju-Ock Molecular Diversity Preservation International 2017 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.18 No.12
<P>Orostachys japonicus A. Berger (), known as Wa-song in Korea, has been reported to exert various biological effects, such as anti-tumor, anti-oxidant, and anti-febrile effects. However, the anti-angiogenic effects of O. japonicus extracts remain to be investigated. In the present study, we demonstrated the anti-angiogenic effects of bioconverted O. japonicus extract (BOE) in Ms-1 mouse endothelial cells and compared them with the bioactivities of O. japonicus extract (OE). BOE, but not OE, were found to exert anti-angiogenic effects, including inhibition of cell migration, cell adhesion, tube formation of Ms-1 cells, and blood vessel formation of matrigel plug assay in vivo. Furthermore, protein levels of phosphorylated Src kinase were lower in BOE-treated cells than in OE-treated cells. Treatment with OE or BOE did not influence cell viability during the experimental period. Bioconverted extract of O. japonicus have anti-angiogenic effects in vitro and vivo, but non-bioconverted extract do not. We suggest that these observed anti-angiogenic effects are caused by the changes in the composition of bioactive compounds in the extracts as a result of biological conversion.</P>
Analysis of chemical changes after WBPH inoculation in the rice leaf
Seul Gi Park,Sopheap Yun,Hyun-Suk Lee,Kyung-Min Kim 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07
This experiment conducted to identify the changes of the response when white-back planthopper (WBPH, Sogatella furcifera), were inoculated in 10 days rice leaves after germination. We confirmed the difference between inoculated and uninfected plants by in the different time period (1day, 1 week, 2 weeks, and 3 weeks after inoculation). Breeding rice and WBPH maintained at 26∼28℃ with 60 % humidity. 3 leaves plants (TN1, Cheongcheong, and Nagdong) were inoculated with 2∼3 instars WBPH. Harvested rice plant samples were completely dried in dark condition and then samples were completely immersed in a solution of methanol for 3 days under darkness. Dissolving in water and then de-fatted three times with hexane. 100 ppm samples were applied to HPLC, eluting with acetonitrile and 0.1 % acetic acid by C18 (5ph column Agilant) and detected at 254 nm. We confirmed the difference of peak using LC/MS/MS (API-2000). The results showed that three weeks from the day of inoculation was increased at the molecular weight 118.1, 264.2 and 364.2.
Lee, Seul-Gi,Joe, Young Ae Elsevier 2018 Biochemical and biophysical research communication Vol.501 No.4
<P><B>Abstract</B></P> <P>Mesenchymal stromal/stem cells (MSCs) have been promising source for regenerative cell therapy in ischemic diseases. To improve efficacy of MSC therapy, various priming methods have been developed, and hypoxic priming has been reported to enhance therapeutic efficacy of MSCs by increasing secretion level of growth factors and cytokines. Recently, it has been reported that bone marrow MSCs primed with hypoxic condition show an increase of autophagy. Here, we addressed whether proangiogenic activity increased by hypoxic condition is associated with autophagy. Wharton's jelly derived MSCs primed with hypoxia showed increase of autophagy with increased hypoxia inducible factor-1α level, and conditioned medium (CM) derived from these cells showed increased levels of migration and tube formation of human umbilical vein endothelial cells (HUVECs) compared to non-primed MSCs-derived CM. Pretreatment with autophagy inhibitor 3-methyladenine or chloroquine prior to exposure of hypoxia resulted in reduction of migration and tube formation of HUVECs. CM obtained under hypoxic condition from MSCs in which autophagy activity was inhibited by ATG5 and ATG7 siRNA treatment also showed decrease of migration and tube formation of HUVECs. Accordingly, secretion levels of angiogenin and VEGF that were markedly increased upon hypoxia exposure was decreased by ATG5/7 knockdown. Therefore, it may be suggested that autophagy plays an important role in hypoxia-driven enhancement of paracrine effect of MSCs.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Hypoxic condition increases autophagy level in mesenchymal stromal/stem cells (MSCs). </LI> <LI> Hypoxic priming of MSCs increases proangiogenic effects of conditioned medium. </LI> <LI> Hypoxia-enhanced proangiogenic effect of MSCs is autophagy-dependent. </LI> <LI> Angiogenin and VEGF are key factors of hypoxia-autophagy-paracrine axis. </LI> </UL> </P>
Lee, Eunji,Lee, Seul-Gi,Park, In-Hyeok,Kim, Seulgi,Ju, Huiyeong,Jung, Jong Hwa,Ikeda, Mari,Habata, Yoichi,Lee, Shim Sung American Chemical Society 2018 Inorganic Chemistry Vol.57 No.11
<P>A 20-membered N<SUB>2</SUB>O<SUB>2</SUB>S<SUB>2</SUB>-macrocycle (<B>L</B><SUP><B>1</B></SUP>) and a 40-membered N<SUB>4</SUB>O<SUB>4</SUB>S<SUB>4</SUB>-macrocycle (<B>L</B><SUP><B>2</B></SUP>) were employed as a [1:1] and a [2:2] cyclization product, respectively, for the preparation of diverse types of supramolecular complexes including a cascade complex. Six complexes (<B>1</B>-<B>6</B>) of the smaller macrocycle <B>L</B><SUP><B>1</B></SUP> including discrete to continuous forms, mono- to heteronuclear, and endo- to exo- and endo/exocoordination were prepared and their coordination modes were discussed systemically. First, the reaction of <B>L</B><SUP><B>1</B></SUP> with CuI in the presence of trifluoroacetic acid afforded an exocyclic 1-D coordination polymer {[(μ<SUB>4</SUB>-Cu<SUB>4</SUB>I<SUB>4</SUB>)(H<B>L</B><SUP><B>1</B></SUP>)<SUB>2</SUB>](CF<SUB>3</SUB>COO)<SUB>2</SUB>}<SUB><I>n</I></SUB> (<B>1</B>). Meanwhile, the reaction of <B>L</B><SUP><B>1</B></SUP> with Cu(ClO<SUB>4</SUB>)<SUB>2</SUB>·6H<SUB>2</SUB>O afforded a typical endocyclic mononuclear complex [Cu<SUP>II</SUP>(<B>L</B><SUP><B>1</B></SUP>)](ClO<SUB>4</SUB>)<SUB>2</SUB>·H<SUB>2</SUB>O (<B>2</B>). In the reactions of <B>L</B><SUP><B>1</B></SUP> with CdX<SUB>2</SUB> (X = Br and I), isostructural sandwich-type complexes [Cd(<B>L</B><SUP><B>1</B></SUP>)<SUB>2</SUB>Br<SUB>2</SUB>] (<B>3</B>) and [Cd(<B>L</B><SUP><B>1</B></SUP>)<SUB>2</SUB>I<SUB>2</SUB>] (<B>4</B>) were isolated. The treatment of <B>L</B><SUP><B>1</B></SUP> with Hg(ClO<SUB>4</SUB>)<SUB>2</SUB> also afforded a sandwich-type complex [Hg(<B>L</B><SUP><B>1</B></SUP>)<SUB>2</SUB>](ClO<SUB>4</SUB>)<SUB>2</SUB> (<B>5</B>). One-pot reaction of <B>L</B><SUP><B>1</B></SUP> with a mixture of HgI<SUB>2</SUB> and CdI<SUB>2</SUB> afforded a dumbbell-type heteronuclear complex {[Cd(<B>L</B><SUP><B>1</B></SUP>)]<SUB>2</SUB>(μ-Hg<SUB>2</SUB>I<SUB>6</SUB>)}[Hg<SUB>2</SUB>I<SUB>6</SUB>] (<B>6</B>), in which the Cd(II) ion occupies the macrocyclic cavity. Further, such two endocyclic Cd(II) complex units are bridged by a square-type (μ-Hg<SUB>2</SUB>I<SUB>6</SUB>)<SUP>2-</SUP> cluster remaining another same cluster separately. The comparative NMR data exhibited a higher affinity of Cd(II) over Hg(II) toward <B>L</B><SUP><B>1</B></SUP>, in the parallel to the situation occurred in the solid state. Meanwhile, complexations of the extra-large macrocycle <B>L</B><SUP><B>2</B></SUP> is more challenging to afford some interesting dimercury(II) coordination products including a cascade complex. In solution, the dimercury(II) perchlorato complex of <B>L</B><SUP><B>2</B></SUP> as a metalloligand shows a preferential binding of dabco (1,4-diazabicyclo[2,2,2]octane), but its dimercury(II) iodo complex has a much smaller affinity for dabco. In order to explain these results, the solid dimercury(II) complexes with different anions [Hg<SUB>2</SUB>(<B>L</B><SUP><B>2</B></SUP>)X<SUB>4</SUB>] (<B>7</B>: X = I, <B>8</B>: X = ClO<SUB>4</SUB>) were prepared and characterized. Further, the dimercury(II) perchlorato complex <B>8</B> reacts with dabco to forms a cascade complex [Hg<SUB>2</SUB>(<B>L</B><SUP><B>2</B></SUP>)(μ-dabco)(ClO<SUB>4</SUB>)<SUB>2</SUB>](ClO<SUB>4</SUB>)<SUB>2</SUB>·2DMF·2ether (<B>9</B>), exhibiting its formation being metal-driven and coordinated anion-regulated. The observed cascade complexation both in solution and solid states is an example of the adaptive guest binding.</P><P>A 20-membered and a 40-membered macrocycles afforded a range of endo/exocoordinated supramolecular complexes including a cascade complex showing anion-regulated organic guest inclusion.</P> [FIG OMISSION]</BR>
Lee, Seul-Gi,Kang, Eun-Ju,Lee, Shim Sung Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.5
A synthesis and cation-induced fluorescent behavior of the carbazole-attached $NO_2S_2$-macrocycle (L) is described and structurally characterized by single crystal X-ray analysis. The photoluminescence spectrum of L in 80% $CH_3CN/CH_2Cl_2$ displays a peak maximum at 431 nm (blue emission). In the metal-induced fluorometric experiment, L showed a drastic chelation-enhanced fluorescence quenching (CHEQ) effect only with $Hg^{2+}$ and $Cu^{2+}$. In ESI-mass study, a 1:1 stoichiometry for complexation of L with $Hg^{2+}$ was confirmed, suggesting the unique sensing behavior of the proposed ligand L due to the selective complexation affinity for $Hg^{2+}$. The observed results indicate that L is a promising turn-off type fluoroionophore for $Hg^{2+}$ and $Cu^{2+}$ detections. Additionally, the $Ag^+$ complex of the precursor macrocycle was prepared and its solid structure was crystallographically characterized.