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- 저자 : Sergio Serna (검색결과 5 건)
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Edgar López-Martínez,Octavio Vázquez-Gómez,Héctor Javier Vergara-Hernández,Sergio Serna,Bernardo Campillo 대한금속·재료학회 2016 METALS AND MATERIALS International Vol.22 No.6
The microhardness and nanohardness of the welding zone of two experimental HSLA steels were determined. The first steel has a microstructure of martensite and bainite, and the second one has a microstructure of quasipolygonalferrite and acicular ferrite. In the bainitic - martensitic steel, softening of the heat affected zone wasobserved. This softening can be attributed to: the formation of polygonal ferrite in the recrystallization subzone,the formation of quasi-polygonal ferrite and the tempering of martensite in the intercritical subzone, andthe tempering of martensite in the subcritical subzone. Besides the softening, with nanoindentation technique, hardeningwas observed at the position where the peak temperature reached the critical temperature Ac1, which canbe attributed to a phenomenon of secondary hardening by precipitation of carbides of alloying elements. In theferritic steel, a softening phenomenon did not appear since there was no martensite in its initial microstructure. Finally, it was noted that both polygonal ferrite and the bainite have similar behavior and nanohardness, thiscoincidence can be attributed to the effect of grain boundary.
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Phenolic–Protein Interactions: Effects on Food Properties and Health Benefits
Nydia E. Buitimea-Cantu´a,Janet A. Gutie´rrez-Uribe,Sergio O. Serna-Saldı´var 한국식품영양과학회 2018 Journal of medicinal food Vol.21 No.2
Phenolic–protein interactions (PPI), which naturally occur in most food systems, are being intentionally designed to enhance functional performance of phenolic compounds (PC). PPI have been primarily associated with changes related to sensorial, nutritional, and nutraceutical features of foods. Furthermore, these interactions affect properties such as astringency, protein digestibility, absorption, and bioavailability of antioxidants. Therefore, new product development should pay attention to these interactions and not only on the concentration of PC. PPI protect PC from degradation due to enzymatic attack or pH changes in the lumen of the intestinal tract. Due to PPI, PC are delivered to the colon where they are metabolized by the microbiota and generate an antioxidant environment. Interactions with proteins also may enhance the antiproliferative activity of PC in some specific tumor cell lines. In this review, the effects of PPI that affect both food properties and health benefits are discussed.
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Najera, Jose Francisco Diaz,Castellanos, Jaime Sahagun,Hernandez, Mateo Vargas,Serna, Sergio Ayvar,Gomez, Omar Guadalupe Alvarado,Verduzco, Clemente Villanueva,Ramos, Marcelo Acosta The Korean Society of Plant Pathology 2018 Plant Pathology Journal Vol.34 No.3
Fruit rot is the principal phytopathological problem of pipiana pumpkin (Cucurbita argyrosperma Huber) in the state of Guerrero. The aims of this research were to 1) identify the causal agent of southern blight on pumpkin fruits by morphological, pathogenic, and molecular analysis (ITS1, 5.8S, ITS2); 2) evaluate in vitro Trichoderma spp. strains and chemical fungicides; and 3) evaluate under rainfed field conditions, the strains that obtained the best results in vitro, combined with fungicides during two crop cycles. Number of commercial and non-commercial fruits at harvest, and seed yield ($kg\;ha^{-1}$) were registered. Morphological, pathogenic and molecular characterization identified Sclerotium rolfsii as the causal agent of rot in pipiana pumpkin fruits. Now, in vitro conditions, the highest inhibition of S. rolfsii were obtained by Trichoderma virens strain G-41 (70.72%), T. asperellum strain CSAEGro-1 (69%), and the fungicides metalaxyl (100%), pyraclostrobin (100%), quintozene (100%), cyprodinil + fludioxonil (100%), and prochloraz (100%). Thiophanate-methyl only delayed growth (4.17%). In field conditions, during the spring-summer 2015 cycle, T. asperellum strain CSAEGro-1 + metalaxyl, and T. asperellum + cyprodinil + fludioxonil, favored the highest number of fruits and seed yield in the crop.
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Janet Alejandra Gutierrez-Uribe,Mauricio Salinas-Santander,Delia Serna-Guerrero,Sergio Roman Othon Serna-Saldivar,Ana Maria Rivas-Estilla,Clara Patricia Rios-Ibarra 한국식품영양과학회 2020 Journal of medicinal food Vol.23 No.1
MicroRNAs (miRNAs) are small molecules of 19–23 nucleotides of RNA that act as regulators of the expression of proteins in eukaryotic cells. Currently, the participation of miRNAs in the development of different types of cancer has been observed. To evaluate the inhibitory effect of kaempferol-3-O-glycoside on the expression of oncological biomarkers, miR31 and miR92a in a colon cancer cell line (RKO) were analyzed. Cells were cultured and treated with 1 mM kaempferol-3-O-glycoside isolated from black bean. Expression levels of miR31 and miR92a were evaluated by real-time PCR using TaqMan probes; in addition, two oncogenes (KRAS and c-MYC) and two tumor suppressors (AMP-activated protein kinase [AMPK] and adenomatous tumors of polyposis coli [APC]) were quantified to validate the biological effects; normalization of expression levels were carried out by 2−ΔΔCt. Results were analyzed by one-way ANOVA. The expression levels of miR31, miR92a, KRAS oncogene, and the c-MYC transcription factor were subexpressed upon 72 h post-treatment with kaempferol-3-O-glycoside compared with the control without treatment (P < .05); in contrast, the tumor suppressor genes AMPK (∼4.85, P = .005) and APC (∼2.71, P = .066) tumor suppressors genes were overexpressed. Our results showed the inhibitory effect of isolated black bean flavonoid kaempferol-3-O-glycoside on cancer biomarkers: miR31 and miR92a; based on our results, this flavonoid may have interesting nutritional, therapeutic, and/or prophylactic applications to combat colon cancer.
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José Francisco Díaz Nájera,Jaime Sahagún Castellanos,Mateo Vargas Hernández,Sergio Ayvar Serna,Omar Guadalupe Alvarado Gómez,Clemente Villanueva Verduzco,Marcelo Acosta Ramos 한국식물병리학회 2018 Plant Pathology Journal Vol.34 No.3
Fruit rot is the principal phytopathological problem of pipiana pumpkin (Cucurbita argyrosperma Huber) in the state of Guerrero. The aims of this research were to 1) identify the causal agent of southern blight on pumpkin fruits by morphological, pathogenic, and molecular analysis (ITS1, 5.8S, ITS2); 2) evaluate in vitro Trichoderma spp. strains and chemical fungicides; and 3) evaluate under rainfed field conditions, the strains that obtained the best results in vitro, combined with fungicides during two crop cycles. Number of commercial and non-commercial fruits at harvest, and seed yield (kg ha-1) were registered. Morphological, pathogenic and molecular characterization identified Sclerotium rolfsii as the causal agent of rot in pipiana pumpkin fruits. Now, in vitro conditions, the highest inhibition of S. rolfsii were obtained by Trichoderma virens strain G-41 (70.72%), T. asperellum strain CSAEGro-1 (69%), and the fungicides metalaxyl (100%), pyraclostrobin (100%), quintozene (100%), cyprodinil + fludioxonil (100%), and prochloraz (100%). Thiophanate-methyl only delayed growth (4.17%). In field conditions, during the spring-summer 2015 cycle, T. asperellum strain CSAEGro-1 + metalaxyl, and T. asperellum + cyprodinil + fludioxonil, favored the highest number of fruits and seed yield in the crop.
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