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Yu Shun Tian,Hyun Joo Jeong,Sang-Do Lee,Seok Heui Kong,Seung-Ho Ohk,Yun-Jung Yoo,Jeong-Taeg Seo,Dong Min Shin,Byung-Wha Sohn,Syng-Ill Lee 대한생리학회-대한약리학회 2010 The Korean Journal of Physiology & Pharmacology Vol.14 No.3
The hyperosmotic stimulus is regarded as a mechanical factor for bone remodeling. However, whether the hyperosmotic stimulus affects 1Ձ, 25-dihydroxyvitamin D<sub>3</sub> (1Ձ,25(OH)<sub>2</sub>D<sub>3</sub>)-induced osteoclastogenesis is not clear. In the present study, the effect of the hyperosmotic stimulus on 1Ձ,25(OH)<sub>2</sub>D<sub>3</sub>- induced osteoclastogenesis was investigated in an osteoblast-preosteoclast co-culture system. Serial doses of sucrose were applied as a mechanical force. These hyperosmotic stimuli significantly evoked a reduced number of 1Ձ,25(OH)<sub>2</sub>D<sub>3</sub>-induced tartrate-resistant acid phosphatase-positive multinucleated cells and 1Ձ,25(OH)<sub>2</sub>D<sub>3</sub>-induced bone-resorbing pit area in a co-culture system. In osteoblastic cells, receptor activator of nuclear factor ՊB ligand (RANKL) and Runx2 expressions were down-regulated in response to 1Ձ,25(OH)<sub>2</sub>D<sub>3</sub>. Knockdown of Runx2 inhibited 1Ձ,25(OH)<sub>2</sub>D<sub>3</sub>-induced RANKL expression in osteoblastic cells. Finally, the hyperosmotic stimulus induced the overexpression of TonEBP in osteoblastic cells. These results suggest that hyperosmolarity leads to the down-regulation of 1Ձ,25(OH)<sub>2</sub>D<sub>3</sub>- induced osteoclastogenesis, suppressing Runx2 and RANKL expression due to the TonEBP overexpression in osteoblastic cells.
Tian, Yu-Shun,Jeong, Hyun-Joo,Lee, Sang-Do,Kong, Seok-Heui,Ohk, Seung-Ho,Yoo, Yun-Jung,Seo, Jeong-Taeg,Shin, Dong-Min,Sohn, Byung-Wha,Lee, Syng-Ill The Korean Society of Pharmacology 2010 The Korean Journal of Physiology & Pharmacology Vol.14 No.3
The hyperosmotic stimulus is regarded as a mechanical factor for bone remodeling. However, whether the hyperosmotic stimulus affects $1{\alpha}$, 25-dihydroxyvitamin $D_3$ ($1{\alpha},25(OH)_2D_3$)-induced osteoclastogenesis is not clear. In the present study, the effect of the hyperosmotic stimulus on $1{\alpha},25(OH)_2D_3$-induced osteoclastogenesis was investigated in an osteoblast-preosteoclast co-culture system. Serial doses of sucrose were applied as a mechanical force. These hyperosmotic stimuli significantly evoked a reduced number of $1{\alpha},25(OH)_2D_3$-induced tartrate-resistant acid phosphatase-positive multinucleated cells and $1{\alpha},25(OH)_2D_3$-induced bone-resorbing pit area in a co-culture system. In osteoblastic cells, receptor activator of nuclear factor ${\kappa}B$ ligand (RANKL) and Runx2 expressions were down-regulated in response to $1{\alpha},25(OH)_2D_3$. Knockdown of Runx2 inhibited $1{\alpha},25(OH)_2D_3$-induced RANKL expression in osteoblastic cells. Finally, the hyperosmotic stimulus induced the overexpression of TonEBP in osteoblastic cells. These results suggest that hyperosmolarity leads to the down-regulation of $1{\alpha},25(OH)_2D_3$-induced osteoclastogenesis, suppressing Runx2 and RANKL expression due to the TonEBP overexpression in osteoblastic cells.