http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Yield-Aware Pareto Front Extraction for Discrete Hierarchical Optimization of Analog Circuits
Seobin Jung,Jiho Lee,Jaeha Kim IEEE 2014 IEEE transactions on computer-aided design of inte Vol.33 No.10
<P>This paper presents an efficient method for extracting a yield-aware Pareto front between two competing metrics of an analog circuit block, with the purpose of performing hierarchical, system-level optimization using the component-level Pareto fronts as meta-models. The proposed method consists of three steps: finding a set of Pareto-optimal design points by tracing them on a discrete grid, estimating the yield distribution of each optimal design point using a control-variate technique, and constructing a yield-aware Pareto front by interpolation. The proposed algorithm is demonstrated on a problem of finding the optimal power allocation among the components composing a clock recovery path to minimize the final clock jitter. The algorithm can estimate the Pareto front of each circuit block within a 2% error, expressing the minimum achievable jitter with 99% yield for different power budgets, while requiring only 600 ~ 1100 Monte-Carlo simulation samples in total.</P>
Variability-Aware, Discrete Optimization for Analog Circuits
Seobin Jung,Jiho Lee,Jaeha Kim IEEE 2014 IEEE transactions on computer-aided design of inte Vol.33 No.8
<P>This paper explores the use of discrete optimization techniques for variability-aware analog circuit synthesis, with an observation that the continuous design space can be effectively covered by a finite number of discrete points when parameter variation is present. Three algorithms are described that can leverage a discretized design space yet mitigate its dimensionality scaling problem: an isotropic discretization scheme, which can fill the neighborhood of any given point with only quadratically-increasing number of nearest neighbors placed at equal distances as the space dimension increases, a stochastic hill-climbing algorithm, which evaluates only a partial set of nearest neighbors yet finds the local optimum at the reduced cost, and an incremental Monte Carlo sampling algorithm, which draws the minimal number of Monte Carlo samples just enough to determine the superior design point during the local search process. These algorithms help in finding the optimal design of analog circuits efficiently. For instance, for a digitally-controlled oscillator example, its discretized design space consists of 5 565 907 points but the optimal point was found by evaluating only 40 design points and running only 21 Monte Carlo simulations per point in average (824 in total).</P>
α-Kleisin subunit of cohesin preserves the genome integrity of embryonic stem cells
Seobin Yoon,Eui-Hwan Choi,Seo Jung Park,김근필 생화학분자생물학회 2023 BMB Reports Vol.56 No.2
Cohesin is a ring-shaped protein complex that comprises theSMC1, SMC3, and α-kleisin proteins, STAG1/2/3 subunits, andauxiliary factors. Cohesin participates in chromatin remodeling,chromosome segregation, DNA replication, and gene expressionregulation during the cell cycle. Mitosis-specific α-kleisinfactor RAD21 and meiosis-specific α-kleisin factor REC8 are expressedin embryonic stem cells (ESCs) to maintain pluripotency. Here, we demonstrated that RAD21 and REC8 were involvedin maintaining genomic stability and modulating chromatinmodification in murine ESCs. When the kleisin subunits weredepleted, DNA repair genes were downregulated, thereby reducingcell viability and causing replication protein A (RPA) accumulation. This finding suggested that the repair of exposedsingle-stranded DNA was inefficient. Furthermore, the depletionof kleisin subunits induced DNA hypermethylation by upregulatingDNA methylation proteins. Thus, we proposed that thecohesin complex plays two distinct roles in chromatin remodelingand genomic integrity to ensure the maintenance of pluripotencyin ESCs.
Elevated expression of exogenous RAD51 enhances the CRISPR/Cas9-mediated genome editing efficiency
Seo Jung Park,Seobin Yoon,Eui-Hwan Choi,Hana Hyeon,Kangseok Lee,김근필 생화학분자생물학회 2023 BMB Reports Vol.56 No.2
Genome editing using CRISPR-associated technology is widelyused to modify the genomes rapidly and efficiently on specificDNA double-strand breaks (DSBs) induced by Cas9 endonuclease. However, despite swift advance in Cas9 engineering,structural basis of Cas9-recognition and cleavage complex remainsunclear. Proper assembly of this complex correlates toeffective Cas9 activity, leading to high efficacy of genomeediting events. Here, we develop a CRISPR/Cas9-RAD51 plasmidconstitutively expressing RAD51, which can bind to singlestrandedDNA for DSB repair. We show that the efficiency ofCRISPR-mediated genome editing can be significantly improvedby expressing RAD51, responsible for DSB repair via homologousrecombination (HR), in both gene knock-out and knock-inprocesses. In cells with CRISPR/Cas9-RAD51 plasmid, expressionof the target genes (cohesin SMC3 and GAPDH) was reducedby more than 1.9-fold compared to the CRISPR/Cas9 plasmidfor knock-out of genes. Furthermore, CRISPR/Cas9-RAD51 enhancedthe knock-in efficiency of DsRed donor DNA. Thus, theCRISPR/Cas9-RAD51 system is useful for applications requiringprecise and efficient genome edits not accessible to HR-deficientcell genome editing and for developing CRISPR/Cas9-mediatedknockout technology.