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RISS 인기검색어
- 검색키워드
- 저자 : Seeni Sooriamuthu (검색결과 2 건)
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Varghese, Reji Joseph,Sooriamuthu, Seeni 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.4
During seed germination trials of Hypericum hookerianum, seedlings of Lake View accession from Palni hills of Southern India segregated into green- (97.44 %) and red-pigmented (2.56 %) types. Seedlings cultured in Murashige and Skoog (1962) basal medium developed into fast growing green and slow growing red plant types in 6 weeks, the latter showing increased concentrations of total phenols, anthocyanins and flavonoids and 19-fold higher concentration of hypericin. Hypocotyls/cotyledons of red seedlings cultured using $2.325{\mu}M$ kinetin (KIN) produced hypericin-rich ($4.38{\pm}0.18$ mg/g DW), stunted (0.5-1.2 cm) shoots which ceased to grow in 8 weeks. Segments (4-6 mm) of these shoots sub-cultured in the dark for 4 weeks followed by 2-week light exposure and repeated subculture enabled mass multiplication of productive ($3.93{\pm}0.06$ mg hypericin/g DW) shoots. Green hypocotyls and cotyledons subjected to 4+2 weak dark-light treatment also produced $9.18{\pm}2.44$ and $4.25{\pm}0.96$ comparable hypericin-rich ($3.73{\pm}0.21$ mg/g DW) shoots. Red and green seedling explants cultured in basal medium in the dark produced $6.82{\pm}0.75$ cm etiolated shoots with reduced leaves which synthesized $2.27{\pm}0.15$ mg hypericin/g DW on illumination. Green cotyledons cultured in the dark using $2.45{\mu}M$ indole-3 butyric acid (IBA) formed calluses which on illumination formed $12.64{\pm}3.8$ productive ($3.86{\pm}0.31$ mg hypericin/g DW) 0.5- to 1.5-cmlong shoot clusters. Phenotypic segregation of seedlings, the ability of both red and green seedling explants to multiply in the dark and produce hypericin on illumination, and IBA-induced indirect shoots producing significant amounts of metabolite compared to wild plants ($0.35{\pm}0.09$ mg/g DW) and green shoot cultures ($0.91{\pm}0.03$ mg/g DW) are new to Hypericum.
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Reji Joseph Varghese,Seeni Sooriamuthu 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.4
During seed germination trials of Hypericum hookerianum, seedlings of Lake View accession from Palni hills of Southern India segregated into green- (97.44 %) and red-pigmented (2.56 %) types. Seedlings cultured in Murashige and Skoog (1962) basal medium developed into fast growing green and slow growing red plant types in 6 weeks, the latter showing increased concentrations of total phenols, anthocyanins and flavonoids and 19-fold higher concentration of hypericin. Hypocotyls/cotyledons of red seedlings cultured using 2.325 lM kinetin (KIN) produced hypericin-rich (4.38 ± 0.18 mg/g DW), stunted (0.5–1.2 cm) shoots which ceased to grow in 8 weeks. Segments (4–6 mm) of these shoots sub-cultured in the dark for 4 weeks followed by 2-week light exposure and repeated subculture enabled mass multiplication of productive (3.93 ± 0.06 mg hypericin/g DW) shoots. Green hypocotyls and cotyledons subjected to 4 ? 2 weak dark–light treatment also produced 9.18 ± 2.44 and 4.25 ± 0.96 comparable hypericin-rich (3.73 ± 0.21 mg/g DW) shoots. Red and green seedling explants cultured in basal medium in the dark produced 6.82 ± 0.75 cm etiolated shoots with reduced leaves which synthesized 2.27 ± 0.15 mg hypericin/ g DW on illumination. Green cotyledons cultured in the dark using 2.45 lM indole-3 butyric acid (IBA) formed calluses which on illumination formed 12.64 ± 3.8 productive (3.86 ± 0.31 mg hypericin/g DW) 0.5- to 1.5-cmlong shoot clusters. Phenotypic segregation of seedlings, the ability of both red and green seedling explants to multiply in the dark and produce hypericin on illumination, and IBAinduced indirect shoots producing significant amounts of metabolite compared to wild plants (0.35 ± 0.09 mg/g DW) and green shoot cultures (0.91 ± 0.03 mg/g DW) are new to Hypericum.
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