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Khurshid Ahmed Mir,Ajmer Singh Dhatt,Jagdeep Singh Sandhu,Satbir Singh Gosal 한국원예학회 2008 Horticulture, Environment, and Biotechnology Vol.49 No.3
In vitro plant regeneration via somatic embryogenesis was attempted using hypocotyl, cotyledon and root derived callus of five elite genotypes of brinjal (Solanum melongena L.) viz. Punjab Barsati, Punjab Sadabahar, Jamuni Gola, PBSR-11 and BB-93C. Somatic embryogenesis in all three explants was induced by using different concentrations of naphthalene acetic acid (NAA) in combination with 0.5 ㎎·L<SUP>-l</SUP> BAP. The highest percent somatic embryogenesis was capitulated with NAA 1.0 ㎎·L<SUP>-l</SUP> from hypocotyl, 1.5 ㎎·L<SUP>-l</SUP> from cotyledon and 2.0 ㎎·L<SUP>-l</SUP> from root explants. The somatic embryos derived from different explants were induced to elongate into shoots on MS basal medium supplemented with 0.25 ㎎·L<SUP>-l</SUP> BAP and regenerated into whole plants. Genotype, explant and genotype x explant interaction showed highly significant effects on embryogenesis. Among the genotypes, BB-93C was the most responsive for somatic embryogenesis, whereas among the explants, hypocotyl yielded the highest percent somatic embryos followed by cotyledon and root. Plants regenerated via somatic embryos were rooted on ½ strength MS basal media in vitro.
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