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- 저자 : Santhosh Mallesh (검색결과 4 건)
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Mallesh Santhosh,Tusan Park 사단법인 한국정밀농업학회 2023 정밀농업과학기술지 Vol.5 No.3
Plants when exposed to stressful conditions, accumulates array of metabolites. Among them proline plays significant and beneficial role as osmolyte, metal chelator and as anti-oxidative defense and regulator molecule. Thus the proline content is one of the potent bio-marker for the early detection of abiotic stress in plant. In this work a microfluidic paper-based electrochemical device (μPED) modified with copper oxide nanoparticles (CuO) decorated reduced graphene oxide (rGO) nanocomposite is utilized to develop disposable system for the detection of proline. Wax and screen printings were utilized to create microfluidic chambers and electrode pattern on paper device, respectively. Portable potentiostat was used for measuring the current in response from the addition of proline. The proposed sensor exhibits excellent electrochemical performance with broad linear responses over a proline concentration ranging from 0.1 mM to 7.5 mM (R2 =0.99) and showed lower detection limit of 0.05 mM at 0.4 V vs. pseudo Ag/AgCl. Excellent electrochemical performance may be attributed to presence of electroactive rGO and catalytic activity towards proline is due to the coordination between proline and copper (II) oxide nanoparticles. These results demonstrate that the proposed paper-based electrochemical sensor can be utilized in disposable and portable way to detect proline content in the abiotic-stressed plants and has potential to be applied in on-site application.
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Paper Microfluidics Based on rGO/Polyaniline Nanofibers for Sensing Pyridoxine
Santhosh Mallesh,박두산 한국농업기계학회 2024 바이오시스템공학 Vol.49 No.1
Purpose This study aims to develop a simple, low-cost, highly disposable paper-based electrochemical device to determine pyridoxine (vitamin B6). Methods Dual-layer paper-based electrochemical devices (PEDs) were fabricated using wax and screen-printing techniques. The electrochemical sensing layer towards pyridoxine detection is prepared by modifying the working electrode with reduced graphene oxide (rGO) and electrodeposited polyaniline (PANI) nanofi bers. Optimization of electrodeposition time was carried out to uniformly coat PANI nanofi bers over rGO-modifi ed PEDs. Surface morphologies of modifi ed electrodes were examined by fi eld emission scanning electron microscopy (FESEM), and PEDs were electrochemically characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Chronoamperometric responses of the modifi ed electrode against pyridoxine were utilized for its quantifi cation. Results Morphological characterization of the modifi ed FESEM indicates PANI’s porous and nanofi ber network over rGO. The electrochemical characterization of the PEDs with CV and EIS reveals that the synergetic combination of PANI and rGO nanostructure possesses excellent electrochemical performance and enhanced electrocatalytic activity towards the oxidation of pyridoxine. The chronoamperometric (CA) responses of the PANI-rGO/PEDs towards pyridoxine showed a broader linear range from 5 to 1100 μM ( R 2 = 0.97) and showed a lower detection limit of 1.43 μM at 0.50 V vs. pseudo-Ag/AgCl. Conclusion The proposed multi-layer PEDs modifi ed with a uniformly formed nanofi ber network of PANI-rGO nanocomposite have the potential to be applied in developing a sensitive sensor for monitoring pyridoxine in a low-cost and disposable way. They are appropriate to be utilized in resource-limited settings.
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Lateral flow immunoassay의 성능개선 연구에 관한 고찰
황수환 ( Suhwan Hwang ),말레시싼토시 ( Mallesh Santhosh ),박두산 ( Tusan Park ) 한국농업기계학회 2023 한국농업기계학회 학술발표논문집 Vol.28 No.2
Lateral flow immunoassay(LFIA)는 신속하고 사용법이 간단한 현장 진단 도구로서 대표적으로 임신 그리고 COVID-19 진단에 사용되고 있으며, 농업 분야에서는 식물 감염병 진단을 위한 감염원(예: Bean mild mosaic virus, Potato virus Y 등)의 검출 시에도 사용되고 있다. 이렇듯 유용한 LFIA의 주요 단점은 상대적으로 복잡하고 긴 시간 동안 수행되는 기존 실험실 진단법(예: Quantitative polymerase chain reaction, Enzyme-linked immunosorbent assay) 대비 검출 한계(Limit of Detection)가 높아서, 샘플에서 낮은 농도의 분석물을 검출하는데 한계가 있다는 것이다. 이를 극복하고자 분석물의 종류에 따라 LFIA의 검출한계를 낮출 수 있는 여러 방법들이 현재에도 지속적으로 연구되고 있는데, 본 포스터에서는 여러 방법들 중 LFIA의 물리적 구조 개선을 통해 민감도(Sensitivity)를 향상시킬 수 있는 방법들에 대해 고찰하고자 하며, 구체적으로 멤브레인의 기공 크기 변화에 따른 샘플의 흐름 특성 변화, 컨트롤 라인 및 테스트 라인의 폭 길이 조정, 멤브레인 상에 특정물질 코팅을 통한 샘플의 흐름 특성 변화, 컨트롤 및 테스트 라인 최적 위치 선정 등의 방법들을 통해 LFIA의 민감도를 향상시키고, 검출 한계를 낮출 수 있는 방법들에 대해 고찰해보고자 한다.
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Chinnadayyala, Somasekhar R.,Park, Jinsoo,Le, Hien Thi Ngoc,Santhosh, Mallesh,Kadam, Abhijit N.,Cho, Sungbo Elsevier 2019 Biosensors & bioelectronics Vol.126 No.-
<P><B>Abstract</B></P> <P>Electrogenerated chemiluminescence (ECL) is an effective method for detecting a wide range of analytes including metal ions, virulent DNA, pathogenic bacteria, tumor cells and glucose. The attractive features of paper including passive liquid transport and biocompatibility are the main two advantages of using paper as a biosensing platform. To achieve key factors in paper-based sensors, the fabrication procedures and the analysis methods are fine tuned to satisfy the requirements of the ultimate–users. Here, we review various ECL signal amplification labels, inexpensive and portable devices, such as rechargeable batteries, which have replaced traditional instrumentation and different light detection technologies used in paper ECL devices. We also highlight the current trends and developments in ECL paper-based microfluidic analytical devices, as well as recent applications of ECL–based detection methods and inexpensive microfluidic devices. We discuss various paper-based devices, including 3D-origami devices, and devices utilizing self-powered and bipolar electrodes. Significant efforts have also been dedicated towards paper based multiplexing analysis (multi-label, and the multi-analyte strategies) and integration of microfluidic lab-on-paper devices with competences for point-to-care diagnostics. This review finally tabulates systematized data on figures of merit and novel types of ECL labels, used for detection of various biomarkers and analytes.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Various Labeling agents for microfluidic paper-based ECL sensors, and representative ECL sensing applications. </LI> <LI> Summary on current trends and developments in light detection technologies and electrical energy supply devices. </LI> <LI> Tabulates systematized data on figures of merit, types of ECL labels, and immobilization supports. </LI> <LI> Multiplexing analysis and integration of microfluidic lab-on-paper devices with competences for point-to-care diagnostics. </LI> </UL> </P>
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