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Sequence Analysis of the Nuclear Matrix - Associated DNA Segments
Lee, Sang Eun,Park, Sang Dai,Lee, Chee Gun,Kim, Chan Kil 한국유전학회 1988 Genes & Genomics Vol.10 No.4
Nuclear matrix isolated from synchronized LP1-1 cells at G1/S boundary digested with DNase 1 to separate a nuclear-matrix(N.M)-associated DNA segments from the rest Through the cloning of the N.M-associated DNA segments (300-500 base pair in size) into a pUC18 vector, 800 clones (named as pMRs) were selected with X-Gal/IPTG and amphiciline. Putative clones harboring replication origin sequence were sequenced and characterized. We found that pMR15 and 16 act as a good template for origins of DNA replication in in vitro replication assay. Furthermore, pMR15 shows A/T richness and has a palindromic sequence. However, pMR62 shows G/C richness (65.7%) and has a homologous sequence to that of Adenovirus replication origin i.e. TGAPyGCC AGG.
Lee, Ho-Sup,Park, Won-Kyu,Son, Hoe-Joo,Lee, Sung-Sook,Kim, Joon-Kyum,Ahn, Soon-Kil,Hong, Chung-Il,Min, Hye-Ki,Kim, Myung-Soo,Myung, Seung-Woon The Pharmaceutical Society of Korea 2004 Archives of Pharmacal Research Vol.27 No.2
The pharmacokinetics of CKD-732 (6-0-4-[dimethyl-aminoethoxy)cinnamoyl]-fumagillolㆍhemioxalate) was investigated in male SD rats and beagle dogs after bolus intravenous administration. The parent compound and metabolites obtained from in vitro and in vivo samples were determined by LC/MS. The main metabolite was isolated and identified as an N-oxide form of CKD-732 by NMR and LC/MS/MS. CKD-732 was metabolized into either M11 or others by rapid hydroxylation, demethylation, and hydrolysis. The blood level following the intravenous route declined in first-order kinetics with $T_{1}$2/$\beta$ values of 0.72-0.78 h for CKD-732 and 0.92-1.09 h for M11 in rats at a dose of 7.5-30 mg/kg. In dogs, $T_{1}$2/$\beta$ values of CKD-732 and M11 were 1.54 and 1.79 h, respectively. Moreover, AUC values increased dose dependently for CKD-732 and M11 in rats and dogs. The CLtot and Vdss did not change significantly with increasing dose, indicating linear pharmacokinetic patterns. The excretion patterns through the urine, bile, and feces were also examined in the animals. The total amount excreted in urine, bile, and feces was 2.13% for CKD-732 and 1.29% for M11 in rats, and 1.58% for CKD-732 and 2.28% for M11 in dogs.
Lee, Hyeon-Yong,Eum, Won-Sik,Kim, Dae-Won,Lee, Byung-Ryong,Yoon, Chang-Sik,Jang, Sang-Ho,Choi, Hee-Soon,Choi, Soo-Hyun,Baek, Nam-In,Kang, Jung-Hoon,Kang, Tae-Cheon,Won, Moo-Ho,Cho, Sung-Woo,Lee, Kil-S Korean Society for Biochemistry and Molecular Biol 2003 Journal of biochemistry and molecular biology Vol.36 No.5
Antioxidant enzymes are scavenger reactive-oxygen intermediates and are involved in many cellular defense systems. We previously reported that a crude extract of Garnoderma lucidum, a medicinally potent mushroom, profoundly increased the catalase gene expression and enzyme activities in mouse livers (Park et al., J. Biochem. Mol. Biol. 34. 144-149, 2001). In this study, we elucidated the detailed mechanism whereby G. lucidum stimulates the catalase activity and expression. The major active fraction was isolated from G. lucidum and methyl linoleate was considered the most major component of the fraction. In order to determine whether methyl linoleate increases mRNA and protein synthesis of catalase, Northern and Western blot analyses were performed in vivo with methyl linoleate-treated mouse liver homogenate after feeding methyl linoleate to the mice. Northern and Western blot analyses of the crude liver homogenates in the mice that were administered methyl linoleate revealed that the expression catalase was significantly increased when compared to the untreated controls. In addition, the catalase protein levels and enzymatic activities increased in the mouse liver homogenates. These results suggest that methyl linoleate that is produced by G. lucidum stimulates the catalase expression at the transcription level.
Lee, Su-Jae,Kang, Kwang-Yong,Jung, Sang-Don,Kim, Jin-Woo,Han, Seok-Kil The Korean Ceramic Society 2000 The Korean journal of ceramics Vol.6 No.3
Highly (h00)-oriented (Ba, Sr)TiO$_3$(BST) thin films were grown by pulsed laser deposition on the perovskite LaNiO$_3$(LNO) metallic oxide layer as a bottom electrode. The LNO films were deposited on SiO$_2$/Si substrates by rf-magnetron sputtering method. The crystalline phases of the BST film were characterized by x-ray $\theta$-2$\theta$, $\omega$-rocking curve and $\psi$-scan diffraction measurements. The surface microsturcture observed by scanning electron microscopy was very dense and smooth. The low-frequency dielectric responses of the BST films grown at various substrate temperatures were measured as a function of frequency in the frequency range from 0.1 Hz to 10 MHz. The BST films have the dielectric constant of 265 at 1 kHz and showed multiple dielectric relaxation at the low frequency region. The origin of these low-frequency dielectric relaxation are attributed to the ionized space charge carriers such as the oxygen vacancies and defects in BST film, the interfacial polarization in the grain boundary region and the electrode polarization. We studied also on the capacitance-voltage characteristics of BST films.
Lee, Jun-Hee,Lee, Ju-Mong,Kim, Joon-Kyum,Ahn, Soon-Kil,Lee, Sang-Joon,Kim, Mie-Young,Jew, Sang-Sup,Park, Jae-Gab,Hong, Chung-Il The Pharmaceutical Society of Korea 1998 Archives of Pharmacal Research Vol.21 No.5
We developed a novel water-soluble camptothecin analobue, CKD602, and evaluated the inhibition of topoisomerase I and the antitumor activities against mammalian tumor cells and human tumor xenografts. CKD602 was a nanomolar inhibitor of the topoisomerase I enzyme in the cleavable complex assay. CKD602 was found to be 3 times and slightly more potent than topotecan and camptothecin as inhibitors of topoisomerase, respecitively. In tumor cell cytotoxicity, CKD602 was more potent than topotecan in 14 out of 26 human cancer cell lines tested, while it was comparable to camptothecin. CKD602 was tested for the in vivo antitumor activity against the human tumor xenograft models. CKD602 was able to imduce regression of established HT-29, WIDR and CX-1 colon tumors, LX-1 lung tumor, MX-1 breast tumor and SKOV-3 ovarian tumor as much as 80, 94, 76, 67, 87% and 88%, respectively, with comparable body weight changes to those of topotecan. Also the therapeutic margin (R/Emax: maximum tolerance dose/$ED-{58}$) of CKD602 was significantly higher than that of topotecan by 4 times. Efficacy was determined at the maximal tolerated dose levels using schedule dependent i.p. administration in mice bearing L1210 leukemia. On a Q4dx4 (every 4 day for 4 doses) schedule, the maximum tolerated dose (MTD) was 25 mg/kg per administration, which caused great weight loss and lethality in <5% tumor bearing mouse. this schedule brought significant increase in life span (ILS), 212%, with 33% of long-term survivals. The ex vivo antitumor activity of CKD602 was compared with that of topotecan and the mean antitumor index (ATI) values recorded for CKD602 were significantly higher than that noted for topotecan. From these results, CKD602 warrants further clinical investigations as a potent inhibitor of topoisomerase I.
THE STABILITY OF THE EQUATION f(x+p) = kf(x)
Lee, Sang-Han,Jun, Kil-Woung Korean Mathematical Society 1998 대한수학회보 Vol.35 No.4
In this paper, we investigate the Hyers-Ulam stability of the (p,k)-MP functional equation.