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Alkaline phosphatase-fused repebody as a new format of immuno-reagent for an immunoassay
Seo, Hyo-Deok,Lee, Joong-jae,Kim, Yu Jung,Hantschel, Oliver,Lee, Seung-Goo,Kim, Hak-Sung Elsevier 2017 Analytica chimica acta Vol.950 No.-
<P><B>Abstract</B></P> <P>Enzyme-linked immunoassays based on an antibody-antigen interaction are widely used in biological and medical sciences. However, the conjugation of an enzyme to antibodies needs an additional chemical process, usually resulting in randomly cross-linked molecules and a loss of the binding affinity and enzyme activity. Herein, we present the development of an alkaline phosphatase-fused repebody as a new format of immuno-reagent for immunoassays. A repebody specifically binding to human TNF-α (hTNF-α) was selected through a phage display, and its binding affinity was increased up to 49 nM using a modular engineering approach. A monomeric alkaline phosphatase (mAP), which was previously isolated from a metagenome library, was genetically fused to the repebody as a signal generator, and the resulting repebody-mAP fusion protein was used for direct and sandwich immunoassays of hTNF-α. We demonstrate the utility and potential of the repebody-mAP fusion protein as an immuno-reagent by showing the sensitivity of 216 pg mL<SUP>−1</SUP> for hTNF-α in a sandwich immunoassay. Furthermore, this repebody-mAP fusion protein enabled the detection of hTNF-α spiked in a serum-supplemented medium with high accuracy and reproducibility. It is thus expected that a mAP-fused repebody can be broadly used as an immuno-reagent in immunoassays.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A human TNF-α (hTNF-α)-specific repebody was selected using a phage display. </LI> <LI> A monomeric alkaline phosphatase (mAP) was genetically fused to the repebody. </LI> <LI> mAP-fused repebody enabled detection of hTNF-α with high sensitivity and accuracy. </LI> <LI> mAP-fused repebody can be widely used as a new immuno-reagent in immunoassays. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
An Analysis of Streambed Changes Downstream of Daecheong Dam
Seo, Hyeong-Deok,Jeong, Sang-Man,Kim, Lee-Hyung,Choi, Kyu-Ho Korean Society of Hazard Mitigation 2008 한국방재학회논문집 Vol.8 No.1
Riverbed change is greatly influenced by artificial factors such as dam construction, gravel collection, and river improvement. This study simulated a long-term bed change based on the GSTARS3 model using actual data from the area downstream of the Geum River Daecheong Dam and compared the estimation with a section of the actual measurement. As a result, it was found that the section of the actual measurement was far lower than the result of the simulation in terms of long-term bed change. While the area downstream of Daecheong Dam displayed approximately an average of 2.29 m of streambed degradation on average while the upper stream area showed approximately 0.63 m of bed degradation over 24 years. In the simulation of the area downstream of Daecheong Dam based on the GSTARS3 model, similar bed degradation was observed. However, a great difference was detected between the result and the actual measurement. According to the cause analysis, the riverbed in the area downstream of Daecheong Dam has continuously degraded due to the dam construction and mass collection of gravel. The mass collection of gravel was the main cause of riverbed change. It was found that about 76% of all riverbed degradation was caused by the mass collection of gravel.
Thermodynamic and kinetic analysis of gas hydrates for desalination of saturated salinity water
Seo, Seong Deok,Hong, Sang Yeon,Sum, Amadeu K.,Lee, Kun-Hong,Lee, Ju Dong,Lee, Bo Ram Elsevier 2019 CHEMICAL ENGINEERING JOURNAL -LAUSANNE- Vol.370 No.-
<P><B>Abstract</B></P> <P>The shortage of fresh water is among the most serious issues in the world. A representative technology to overcome the problem is desalination, but most conventional methods (RO membrane or thermal distillation) have been focused on the treatment of relatively low salinity water, such as seawater or brackish water. To strengthen water security, in this study, we introduce a possibly economic technology for desalination of high salinity water (over-saturated concentration, in this study, a 30 wt% NaCl system) via gas hydrate formation by coupling LNG waste cold energy. First, the thermodynamic effects of NaCl on CH<SUB>4</SUB> (methane), SF<SUB>6</SUB> (sulfur hexafluoride), and HFC-134a hydrates were investigated. Based on the phase equilibrium of each hydrate, experimental pressures for kinetic experiments were selected under vapor pressure boundaries as follows: 4.5 MPa for CH<SUB>4</SUB>, 0.75 MPa for SF<SUB>6</SUB>, and 0.16 MPa for HFC-134a at 258.15 K (assuming the use of LNG waste cold energy). The results of the formation kinetics on the basis of gas moles consumed for hydrates showed the order CH<SUB>4</SUB> > HFC-134a > SF<SUB>6</SUB>; however, after considering the hydration numbers and structures for each hydrate, surprisingly, the conversion rate of water to gas hydrates showed the order HFC-134a > CH<SUB>4</SUB> > SF<SUB>6</SUB>, even though the experimental pressure condition for HFC-134a was very mild (0.16 MPa) compared to CH<SUB>4</SUB> (4.5 MPa). For this interesting phenomenon, we suggest a possible mechanism through visual observations during hydrate formation. We believe these thermodynamic, kinetic, and morphological results show potential as an alternative desalination technology, especially for saturated salinity water, with lower energy consumption.</P> <P><B>Highlight</B></P> <P> <UL> <LI> Thermodynamics and kinetics of gas hydrates were studied in saturated NaCl system. </LI> <LI> Using LNG waste cold energy was assumed for hydrate formation kinetics experiments. </LI> <LI> HFC-134a hydrates in saturated NaCl system formed at 0.16 MPa and 258.15 K. </LI> <LI> The kinetics of hydrates conversion showed the order HFC-134a > CH<SUB>4</SUB> > SF<SUB>6</SUB>. </LI> <LI> HBD process with HFC-134a is possibly economic in over-saturated salinity system. </LI> </UL> </P>
( Sang Keun Lee ),( Eun Kyoung Jeon ),( Yu Jin Kim ),( Sam Hwa Seo ),( Chang Deok Kim ),( Jong Soon Lim ),( Jeung Hoon Lee ) 대한피부과학회 2009 Annals of Dermatology Vol.21 No.3
Background: Psoriasis is a chronic inflammatory skin disease that affects approximately 1~3% of the general population. Objective: We performed cDNA microarray analysis with using the dendrimer labelling method to investigate the gene expression profile in the peripheral blood mononuclear cells (PBMCs) of psoriatic patients. Methods: The peripheral blood mononuclear cells of 5 patients with psoriasis and 8 control subjects were used in the gene expression analyses of psoriasis. Results: We identified 212 differentially expressed genes that showed at least a two-fold induction and/or reduction in psoriatic patients. Among those, 63 genes, including CD44, CD56 and IL7R, were induced, while 139 genes, including the sphingosine kinase 1 and p16-INK genes, were reduced in the psoriatic patients. Conclusion: We can speculate that these genes may have a role for the pathogenesis of psoriasis via their affecting different cellular functions. Our results suggest a possible mechanism by which activated immune cells migrate from the blood to the skin in psoriatic patients, and we provide novel putative targets for developing drugs to treat psoriasis. (Ann Dermatol 21(3) 237~242, 2009)
Topology optimization using spline finite element method and trimmed surfaces
Sung-Kie Youn,Yu-Deok Seo,Hyun-Jung Kim 대한기계학회 2010 대한기계학회 춘추학술대회 Vol.2010 No.3
In the present work, a new topology optimization based on spline finite element method(FEM) and trimmed spline surfaces is proposed. For a new topology optimization based on spline FEM, trimming techniques are employed to represent arbitrary complex topologies in spline surfaces. Trimmed surface analysis which uses trimming information of trimmed surfaces for numerical analysis is employed to treat topologically complex spline surfaces. The boundaries are optimized by changing the positions of control points of the surface and those of trimming curves. As a reasonable measure for topological change, the selection criteria, which are defined by the ratio of the sensitivity on an objective function to that on a constraint, are employed for creation of a new inner front. Inner front merging algorithm is also implemented for high topological flexibility in the proposed approach. The proposed spline based topology optimization is demonstrated by applying some benchmarking problems.
Cultivable Microbial Diversity in Domestic Bentonites and Their Hydrolytic Enzyme Production
Seo, Dong-Ho,Cho, Eui-Sang,Hwang, Chi Young,Yoon, Deok Jun,Chun, Jeonghye,Jang, Yujin,Nam, Young-Do,Park, So-Lim,Lim, Seong-Il,Kim, Jae-Hwan,Seo, Myung-Ji The Korean Society for Microbiology and Biotechnol 2019 한국미생물·생명공학회지 Vol.47 No.1
We have isolated and identified 72 bacterial strains from four bentonite samples collected at the mining areas located in Gyeongsangbuk-do, Republic of Korea, and measured their hydrolytic enzyme (${\alpha}$-amylase, protease, and cellulase) activities to identify the isolates with industrial-use potential. Most of the isolates belonged to the Bacillaceae, with minor portions being from the Paenibacillaceae, Micrococcaceae, and Bacillales Family XII at the family level. Of the strains isolated, 33 had extracellular ${\alpha}$-amylase activity, 30 strains produced cellulase, and 35 strains produced protease. Strain MBLB1268, having the highest ${\alpha}$-amylase activity, was identified as Bacillus siamensis ($0.38{\pm}0.06U/ml$). Bacillus tequilensis MBLB1223, isolated from Byi33-b, showed the highest cellulase activity ($0.26{\pm} 0.04U/ml$), whereas Bacillus wiedmannii MBLB1197, isolated from Zdb130-b, exhibited the highest protease activity ($54.99{\pm}0.78U/ml$). These findings show that diverse bacteria of the Bacillaceae family adhere to and exist in bentonite and are potential sources of industrially useful hydrolytic enzymes.
Expression of Neutrophil Gelatinase-Associated Lipocalin in Skin Epidermis
Seo, Seong Jun,Ahn, Ji-Young,Hong, Chang-Kwun,Seo, Eun-Young,Kye, Kyung-Chae,Lee, Woong-Hee,Lee, Sang-Keun,Lim, Jong-Soon,Hahn, Myong-Joon,Kjeldsen, Lars,Borregaard, Niels,Kim, Chang Deok,Park, Jang-K Elsevier 2006 The Journal of investigative dermatology Vol.126 No.2