Impact of edaphic factors and nutrient management on the hepatoprotective efficiency of Carlinoside purified from pigeon pea leaves: An evaluation of UGT1A1 activity in hepatitis induced organelles

Das, Subhasish,Teja, K. Charan,Mukherjee, Sandip,Seal, Soma,Sah, Rajesh Kumar,Duary, Buddhadeb,Kim, Ki-Hyun,Bhattacharya, Satya Sundar Elsevier 2018 Environmental research Vol.161 No.-

<P><B>Abstract</B></P> <P>Carlinoside is a unique compound well-known for its excellent curative potential in hepatitis. There is a substantial research gap regarding the medicinal use of carlinoside, as its concentrations are greatly variable (depending on locality). We cultivated <I>Cajanus cajan</I> using vermicompost as a major organic amendment at two locations (Sonitpur and Birbhum) with different soil types, but identical climate conditions. Sonitpur soils were richer in soil organic C (SOC), enzyme activation, and N/P content than Birbhum. However, vermi-treatment improved many soil properties (bulk density, water retention, pH, N/P/K, and enzyme activity) to narrow the locational gap in soil quality by 15–28%. We also recorded a many-fold increment in SOC storage capacities in both locations, which was significantly correlated with carlinoside, total phenol, and flavonoid contents in <I>Cajanus</I> leaves. This significantly up-regulated the carlinoside induced expression of the bilirubin-solubilizing UGT1A1enzyme in HepG2 cell and rat liver. Leaf extracts of vermicompost-aided plants could cure hepatitis in affected rat livers and in the HepG2 cell line. Accordingly, vermi-treatment is an effective route for the growth of <I>Cajanus</I> as a cash crop for biomedical applications and can produce a concurrent improvement in soil quality.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Soil organic C primarily induces carlinoside biosynthesis in <I>Cajanus cajan</I>. </LI> <LI> Vermicompost elevates the medicinal value of <I>Cajanus cajan</I> against hepatitis. </LI> <LI> Vermi-treated <I>Cajanus</I> yield carlinoside stably irrespective of growing location. </LI> <LI> Vermi-treated leaf extracts expedited bilirubin solubilization in rat liver. </LI> </UL> </P>

Studies of dissipative standing shock waves around black holes

Das, Santabrata,Chakrabarti, Sandip K.,Mondal, Soumen Blackwell Publishing Ltd 2010 MONTHLY NOTICES- ROYAL ASTRONOMICAL SOCIETY Vol.401 No.3

<P>ABSTRACT</P><P>We investigate the dynamical structure of advective accretion flow around stationary as well as rotating black holes. For a suitable choice of input parameters, such as accretion rate <IMG SRC='http://onlinelibrary.wiley.com/store/10.1111/j.1365-2966.2009.15793.x/asset/equation/MNR_15793_mu1.gif?v=1&s=1f863aabc4afe4e815441d3668ba8bef331dbb46'/> and angular momentum (λ), a global accretion solution may include a shock wave. The post-shock flow is located at a few tens times the Schwarzchild radius and is generally very hot and dense. This successfully mimics the so-called Compton cloud, which is believed to be responsible for emitting hard radiation. Owing to the radiative loss, significant energy is removed from the accreting matter and the shock moves forward towards the black hole in order to maintain the pressure balance across it. We identify the effective area of parameter space <IMG SRC='http://onlinelibrary.wiley.com/store/10.1111/j.1365-2966.2009.15793.x/asset/equation/MNR_15793_mu2.gif?v=1&s=390e74728c22c2f001e31788bfde14ab9deeb019'/> that allows accretion flows to have some energy dissipation at the shock (Δε). As the dissipation is increased, the parameter space is reduced and finally disappears when the dissipation reaches a critical value. The dissipation has a profound effect on the dynamics of post-shock flow. By moving forward, an unstable shock, the oscillation of which causes quasi-periodic oscillations (QPOs) in the emitted radiation, will produce oscillations of high frequency. Such an evolution of QPOs has been observed in several black hole candidates during their outbursts.</P>

Stabilizing Adaptive Controller for Uncertain Dynamical Systems: An LMI Approach

Sandip Ghosh,Sarit K. Das,Goshaidas Ray 제어·로봇·시스템학회 2009 International Journal of Control, Automation, and Vol.7 No.2

Adaptive stabilization of a class of linear systems with matched and unmatched uncertainties is considered in this paper. The proposed controller indeed stabilizes the uncertain system for any positive values of its non-adaptive gain that may be tuned to enhance dynamic response of system. The performance of uncertain system along with the Algebraic Riccati Equation that arises from the adaptive stabilizing controller is now formulated as a multi-objective Linear Matrix Inequality optimization problem. The decay rate and a factor governing the ultimate bound of the system states are considered to characterize the closed loop system performance. Finally, the effectiveness of the proposed controller is illustrated via stabilizing a mass-spring system.

Sequence conservation and divergence in miR164C1 and its target, CUC1, in Brassica species

Karuna Kusumanjali,Sandip Das,Gunjan Kumari,P. S. Srivastava 한국식물생명공학회 2012 Plant biotechnology reports Vol.6 No.2

An emerging aspect of plant development and adaptation studies is microRNA (miRNA)-mediated gene regulation and to understand post-transcriptional gene silencing (PTGS) dynamics. Studies performed within an evolutionary context, such as specific plant lineages and genome status (e.g. ploidy level) can highlight the selection pressure and constraints guiding the evolutionary trajectory of regulatory modules. We have investigated conservation and allelic diversity in one such module composed of CUC1 (CUP-SHAPED COTYLEDON 1), a NAC domain family member, and miR164C, within Brassica species. Ancestral CUC1 sequences, including those belonging to Amborellales, Nymphaeales and Austrobaileyales, harbour miR164 binding sites, implying an ancient nature of this regulatory module. We found that sequence and length polymorphisms occurred in the miR164C precursor of diploid and amphidiploid Brassica species, although the 21-nt mature sequence was invariant. In contrast, the target sequence in CUC1 exhibited polymorphism at the ‘‘seed’’and ‘‘cleavage’’ site, otherwise governed by stringent rules of pairing for successful PTGS, implying a ‘‘relaxed’’selection pressure on CUC1. Further studies are needed to improve our understanding of the functional implications of the polymorphism and single nucleotide polymorphisms that constitute allelic variation at the region responsible for PTGS within Brassica species, in context of ploidy level,and to unravel the evolutionary dynamics of the miR164C–CUC1 regulatory module within the plant kingdom.

Condition assessment of bridge pier using constrained minimum variance unbiased estimator

Tamuly, Pranjal,Chakraborty, Arunasis,Das, Sandip Techno-Press 2020 Structural monitoring and maintenance Vol.7 No.4

Inverse analysis of non-linear reinforced concrete bridge pier using recursive Gaussian filtering for in-situ condition assessment is the main theme of this work. For this purpose, minimum variance unbiased estimation using unscented sigma points is adopted here. The uniqueness of this inverse analysis lies in its approach for strain based updating of engineering demand parameters, where appropriate bound and constrained conditions are introduced to ensure numerical stability and convergence. In this analysis, seismic input is also identified, which is an added advantage for the structures having no dedicated sensors for earthquake measurement. First, the proposed strategy is tested with a simulated example whose hysteretic properties are obtained from the slow-cyclic test of a frame to investigate its efficiency and accuracy. Finally, the experimental test data of a full-scale bridge pier is used to study its in-situ condition in terms of Park & Ang damage index. Overall the study shows the ability of the augmented minimum variance unbiased estimation based recursive time-marching algorithm for non-linear system identification with the aim to estimate the engineering damage parameters that are the fundamental information necessary for any future decision making for retrofitting/rehabilitation.

Computerised Analysis of Rankine Cycle Leading to a Correlation with the Dimensionless Number RN

Rajat Chattopadhyay,Subhas Dutta Gupta,Sandip Das 대한기계학회 1988 KSME/JSME THERMAL and FLUID Engineering Conference Vol.1 No.1