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Azhar Rashid Lone,Nalini Tiwari,Samrendra Singh Thakur,Oren Pearlson,Tomáš Pavlíček,Shweta Yadav 국립중앙과학관 2020 Journal of Asia-Pacific Biodiversity Vol.13 No.2
The present investigation was carried out on the earthworms collected from the reserve forests ofMeghalaya, which is a part of the North Eastern Region of India, a hot spot of biodiversity to assessdiverse earthworms. We adopted integrative approaches involving morphoanatomical taxonomy andcytochrome oxidase 1 sequences using DNA barcoding technique to discriminate different species ofKanchuria genus. The exploration revealed the presence of six Kanchuria species among which four arenew: Kanchuria daribokgrensis sp nov, Kanchuria karorensis sp nov, Kanchuria makhulensis sp nov, andKanchuria mohiskulensis sp nov. Moreover, the species of Kanchuri turaensis and Kanchuri octotheca wereadditionally identified. The maximum intraspecific and minimum interspecific divergence of six Kanchuriaspecies recorded were 6.11% and 14.85%, respectively. The neighbor-joining tree was constructedusing the K2P substitution model, and species discovery was achieved by Automatic Barcode Gap Discoveryand Barcode Index Number analysis. The study presented the detailed descriptions and illustrationsof six species including four new species of Kanchuria supplemented with their DNA barcodesfrom Meghalaya (North Eastern Region) to facilitate future species identification.
Interrogation of earthworm (Clitellata: Haplotaxida) taxonomy and the DNA sequence database
Nalini Tiwari,Azhar Rashid Lone,Samrendra Singh Thakur,Shweta Yadav 국립중앙과학관 2021 Journal of Asia-Pacific Biodiversity Vol.14 No.1
The DNA sequence database assists us with great potential in species identification because it is practicaland affordable to perform. More often, the identification using molecular data helps to identifymorphologically variable individuals of same species, including juvenile specimens. In case of earthworms,the classical standards of taxonomy are mainly based on genital structures, while the collectionof the sexually agile worm is difficult. Hence, typical barcode marker cytochrome c oxidase subunit 1 wasused to explore species richness of the study area. From taxonomic relationship, the valid identificationof voucher specimens was taken into consideration for characterization. The species richness of earthwormsp. viz., Metaphire houlleti, Perionyx sp., Drawida minuta, Eudichogaster prashadi, Lennogasterpusillus, Octochaetona beatrix, and Octochaetona prashadi were recorded and analyzed. Less than 1%divergence within species was found, whereas divergence between species was greater than 15% in allcases. The phylogenetic tree(s) was constructed using the best-fitted substitution model of evolution andspecies delimitation by Automatic Barcode Gap Discovery, operational taxonomic units, and networkanalysis. The prospective and limitations of molecular taxonomy and the vital role of publicly availablenucleotide sequence databases were reviewed to inventor earthworm diversity. The study shows theuniversality of single locus persists utopian and the use of other typical loci may strengthen earthwormdiversity assessment.