산소유리라디칼이 뇌조직 미크로좀분획의 Na⁺-K⁺-ATPase 활성도에 미치는 영향

오세문(Sae Moon Oh)손영숙(Young Sook Son),최길수(Kil Soo Choi),임정규(Jung Kyoo Lim),정명희(Myung Hee Chung) 대한약리학회 1982 대한약리학잡지 Vol.18 No.2

중추신경계의 혈관폐쇄 또는 충격손상에 의한 허혈병소에서 진행되는 병리적 변화에 산소유리라디칼이 중요한 역할을 할 것으로 시사되고 있다. 저자는 산소유리라디칼이 뇌조직에 미치는 영향 중 특히 신경세포 정지막전위 유지에 중요한 Na⁺-K⁺-ATPase의 활성도에 미치는 영향을 관찰하기 위하여 산틴산화효소 반응계와 뇌조직미크로좀을 이용하여 본 연구를 시행하였다. 미크로좀분획(microsomal fraction)을 산틴과 산틴산화효소와 함께 반응시켰을 때, 분획의 Na⁺-K⁺-ATPase의 활성도는 현저한 불활성화를 보인 반면, Mg⁺⁺-ATPase의 활성도는 별로 영향을 받지 않았다. 이 불활성화는 산틴과 신틴산화효소 중 어느 한 물질이라도 반응계에 존재하지 않는 경우에는 나타나지 않았고, 두 물질이 같이 반응계에 존재할 때 나타났다. 산틴과 산틴산화효소의 반응에서 생성되는 산소유리라디칼들 중, 어떤 것이 Na⁺-K⁺-ATPase불활성화에 관계하고 있는가를 알아보기 위하여, 산수유리라디칼 각각에 대하여 제독작용을 가진 효소나 화학물질을 사용하여 불활성화의 저해유무를 관찰하였다. O^-₂⋅의 제독효소인 superoxide dismutase, H₂O₂의 제독효소인 catalase와 ¹O₂의 제독물질인 1,4-diazabixyclo(2,2,2)octane을 각각 사용하였을 때, 이들 물질들이 농도에 비례하여 Na⁺-K⁺-ATPase의 불활성화가 저해되었다. 그러나 OH⋅의 제독물질인 mannitol은 뚜렷한 효과를 보이지 못했다. 이상의 결과는 산소유리라디칼들 중 O^-₂⋅, H₂O₂ 및 ¹O₂가 Na⁺-K⁺-ATPase의 불활성화에 관계하고 있고, OH⋅는 거의 관계하지 않는다는 것을 시사하여 주었다. 이로 미루어, 산소유리라디칼에 의한 뇌조직 Na⁺-K⁺-ATPase의 불활성화는 뇌 허혈병소에서 관찰되는 신경세로의 기능적 장해를 유발시키는 한 요인으로 사료되었다. The effects of xanthine-xanthine oxidase reaction on brain microsomal Na⁺-K⁺-ATPase activity were studied to see possible involvement of oxygen free radicals in pathologic change occurring in ischemic state of CNS accompanied by cerebral vascular occlusion or impact injury. When microsomal fraction was incubated with xanthine ana xanthine oxidase, Na⁺-K⁺-ATPase activity of the fraction was markedly inactivated (80% inactivation) whereas btssl Mg⁺⁺-ATPase was much less sensitive (less than 10% inactivation) compared to that of Na⁺-K⁺-ATPase. The inactivation was observed only in the presence of both xanthine and xanthine oxidase, not either of them alone, and the extent of inactivation was dependent on the concentration of xanthine. In an attempt to determine which of the oxygen species was responsible for the inactivation, the ability of various scavengers to overcome the inactivation was tested. Superoxide dismutase, catalase and 1,4-diazabicyclo(2,2,2)octane were shown to reverse the inactivation of the ATPase in dose-dependent manner. In contrast, mannitol as well as other OH⋅quenchers were ineffective in limiting oxygen radical-induced inactivation. Thus OO^-₂⋅, H₂O₂ and ¹O₂ were implicated to be mediators involved in the inactivation. Since oxygen radicals are suspected as being a cause of the peroxidative damaging process in train ischemia, the ATPase inactivation by oxygen radicals may be a possible contributing factor which gives rise to functional derangement of nerve cells observed in the pathologic process.

Sabah Underframe Wagon의 强度에 관한 硏究

吳世旭,周原植,蔣伯善 동아대학교 공과대학 부설 한국자원개발연구소 1981 硏究報告 Vol.5 No.2

Recently there have been many studies on developing trains, automobiles, etc. into the higher speed and lighter weight condition. In order to get the lighter weight of a wagon, conventionally the cast steel structure was used, but nowadays the welded structure of rolled steel has replaced it for the manufacturing. These structures have many merits but some defects. so that, there would be able to happen the damage of life and property on account of some un-anticipated accidents in spite of the safety design of the structures. Therefore, for safety operation, the stress analysis by using strain gauge method should be taken on the developed test car to find out any weak points and strengthen them additionally. This study deals with the strength test by strain gauge method for Sabah underframe wagons designed and made in the Korea Shipbuilding & Engineering Corp. And the stress and deflections of the underframe were measured by strain gauge method, the strength was analyzed and the safety was considered to find out weak points and to carry out additional strengthening design and manufacturing.

SFA55材의 機械的性質 및 비틀림疲勞特性에 미치는 熱處理溫度의 影響

吳世旭,金晋坤,朴正洙 동아대학교 공과대학 부설 한국자원개발연구소 1984 硏究報告 Vol.8 No.2

Nowadays, SFA55 has been used to train frame widely It is necessary that mechanical properties and fracture behavior of SFA55 should be evidently cleared in order to prevent fracture accident of train frame previously. For impact test specimens SFA55 have been treatted by two methods; water-quenched at 880℃, tempered at various temperatures, and for twisting fatigue test specimens; normalized at 880℃, tempered at 550℃ after quenched at 880℃. Impact test has been performed by charpy impact tester (30Kgf-m) and twisting test has been conducted by schenk type twisting tester. After tests, fracture behaviors and effects of heat treatments have been investigated with Scanning Electron Microscope. The main results obtained were as follows; 1) Impact values of SFA55 have increased, but hardnesses have decreased with the tempering-temperature increasing. That is, ductility has been increased. 2) It has been generally reported that the difference of fatigue limits between normalized materials and quenched-tempered materials was almost negligible, but it has been observed that the fatigue limit of the later was greater than that of the former. 3) In the impact test specimen, the fractured surface at vicinity of U-notch was ductile dimple style and at nearly center area was brittle cleavage fracture. 4) At the center area of the impact test specimen the dimple style has been increased with the tempering temperature. 5) The normalized materials of the twisting fatigue test specimen has smooth and clean striations but the quenched-tempered materials has discontinuous and irregular striation.

대학스키수업 참가자들의 내적 동기와 몰입과의 관계에 관한 연구

오세숙,오세이 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.5

The primary purpose of this study was to investigate the relationship between intrinsic motivation and flow experiences for university students who attending winter ski class. The results of many past researches showed that people who had higher intrinsic motivation should had higher flow experiences. Based on these researches' results, this research studied on university students' who attending winter ski class. The study was based on a sample of 320 university students who attending 2003-2004 season winter ski class. Intrinsic Motivation Inventory (Ryan, 1982) and 11 characteristics of flow (Jackson & Roberts, 1992) were used in survey. The result showed all five intrinsic motivation factors were significant at the level of .05: Pressure/Tension(.00); Effort(.00); Perceived Competence(.00); Perceived Choice(.00); Interesting/Enjoyment(.04). This results indicated that people who had higher intrinsic motivation should had higher flow experiences.

국제 태권도 연맹에 관한 고찰

오세용,한창효 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.3

This research is about creation background of I,T,F,(International Taekwondo Federation) and relationship between I.T.F.(International Taekwondo Federation) and choi hong hi futhermore, unification with W,T,F(World Taekwondo Federation), The conclusion of this research is as follows. First, Choi hong hi is the one who created word of "Taekwondo" and he spread this sports in north korea from the very beginning, Taekwondo was spreaded through out the world from north korea, Until he died in june 15, 2002, he attended various Taekwondo seminars and taught Taekwondo. Second, I,T,F(International Taekwondo Federation) was created based on political background and the first demonstration team was the the leading role of bridge to create I,T.F,(International Taekwondo Federation), As of today, they organize numerous world Taekwondo contests and demonstration teams to advertise Taekwondo to the world. Third, there is a big controversy for the unification of I,T,F,(International Taekwondo Federation) and W,T,F(World Taekwondo Federation)., But these 2 groups should unify within short period of time to make quality and positive growing.

Formocresol과 水酸化 Calcium 및 Glutaraldehyde가 切斷施術된 齒髓에 미치는 影響에 關한 實驗的 硏究

吳世潤,韓澤善 고려대학교 의과대학 1984 고려대 의대 잡지 Vol.21 No.1

This experimental study was performed to compare the effects of formocresol, calcium hydroxide and glutaraldehyde on the vitally amputated pulp tissues. 8 premolars of each sample, total of 64 from 8 young sound dogs were pulpotomized with one of the following 8 different methods. i.e., 5 minutes application of formocresol on the amputated pulp surface and capping with zinc oxide eugenol paste mixed with formocresol, 5 minutes application of formocresol and capping with calcium hydroxide mixed with or without formocresol, 4 days application of formocresol and capping with either zinc oxide eugenol paste or calcium hydroxide, direct capping with calcium hydroxide only or calcium hydroxide mixed with formocresol, and 5 minutes application of 2% glutaraldehyde and capping with calcium hydroxide mixed with 2% glutaraldehyde. 3 dogs were sacrificed 3 weeks later and the other 5 were sacrificed 200 day later respectively, and all the pulpotomized teeth were prepared for histological findings. The obtained results were as follows; 1. In the pulp tissues that were treated with formocresol and capped with zinc oxide engenol mixed with or without formocresol, no dentin bridge was observed and inflammatory response seemed to progress deeper leading to necrosis of the pulp sooner or later. 2. In the pulp tissues that were treated with formocresol for 5 minutes and capped with calcium hydroxide mixed with or without formocresol, or directly capped with calcium hydroxide mixed with formocresol, relatively mild inflammatory reaction was observed with complete dentin bridge formation and the vitality of the remaining pulp tissues appeared to be slightly degenerated. 3. In the pulp tissues that were treated with formocresol for 4 days, generally degenerative and destructive change was severer than in the ones that were treated for 5 minutes. 4. In the pulp tissues that were capped with calcium hydroxide, more favorable tissue reaction was observed when used with formocresol than in the ones where formocresol was not used. 5. In the pulp tissues that were treated with 2% glutaraldehyde and capped with calcium hydroxide mixed with 2% glutaraldehyde instead of formocresol, generally compact dentin bridge was formed and the vitality of the remaining pulp tissues was preserved for a long time relatively with less inflammatory response. 6. In the early stage of osseous structure formation, it appeared relatively spongeous structure showing many spaces, forming tunnels between capping material and the remaining pulp. In the favorable cases, the space was more rapidly closed and dentin bridge was densely added along the lower border of osseous structure covering the remaining pulp, on the other hand, as the formation of dense dentin bridge was retarded, it was more spacious and thus the inflammatory stimulus penetrating deeper and destroying the remaining pulp more severely. 7. The vitality of remaining pulp tissue, pulpotomized with most of the usual methods, seemed to be declined so the author suppose that further more improved method would be necessary.

남북한 대학생 교류방향의 모색

吳世胤 湖南大學校 1997 호남대학교 학술논문집 Vol.18 No.1

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構造用鋼의 熱處理溫度에 따른 衝擊特性에 관한 硏究

吳世旭,安光珠 동아대학교 공과대학 부설 한국자원개발연구소 1983 硏究報告 Vol.7 No.1

It is not enough to determine the impact property by the absorbed impact energy only, so the measurement of impact load has been urged. In impact test the charpy specimen with U type notch were used to determine the impact properties. The specimens were tempered from 100℃ to 800℃ after quenching. Experiments were carried out for such characteristics as impact load, impact value, loading, period, deflection and reduction rate of loading speed. The experimental results are summarized as follows; 1. By comparing with tempering temperature at maximum load between impact and static, tempering temperature of impact specimen is higher than those of the static specimen. 2. Static ratio(γ) between impact and static loading are; γ=1.17~1.60 for spring steel. γ=1.02~1.23 for SM20C steel. 3. The transition tempering temperature in impact value and velocity reduction rate is 300℃.

複合組織鋼의 摩擦 熔接部의 疲勞破壞特性에 관한 硏究

吳世旭,姜鎬珉,兪在煥,林東哲 東亞大學校 1988 東亞論叢 Vol.25 No.1

This study has been carried out to investigate the characteristics of fatigue crack of welded zone and heat affected zone of friction welded dual phase steel. The results obtained from the experiments are summarized as follows. Microstructure of welded zone is changed to ferrite and pearlite from ferrite and martensite by wel-ding heat. Micro-vickers hardness of welded zone is higher than that of base metal zone and heat affec-ted zone. Micro-vickers hardness of heat affected zone is lower than that of welded zone and microstructure of heat affected zone is changed to fine martensite and ferrite by heating cycle of welding. Fatigue limits of heat affected zone is a little higher than that of welded zone and base metal zone. There are no difference of fatigue limit of base metal zone and welded zone. Fatigue strength of welded zone is similar to that of base metal zone. At high △K region(△K>19MPa·??), fatigue crack propagation velocity of heat affected zone is slower than that of welded zone and base metal zone, and the difference of fatigue crack propagation velocity is smaller than that of high △K region. The crack occurs to the notch in heat affected zone first, but the final fracture surface is the surface of welded zone. It is abvious that heat affected zone of friction welded dual phase steel superior to welded zone and base metal zone in fatigue strength and resistance of fatigue crack propagation. The above mentioned facts are contrary to theory that heat affected zone of general metals is weaker than welded zone and base metal zone.

한국 자동차사고의 요인분석

吳世胤 湖南大學校 1996 호남대학교 학술논문집 Vol.17 No.1

Korea is notorious for its road traffic accidents, so 10,087 were dead in 1994. But there has been a dispute on what is the major cause of the traffic accidents. Some people say the major cause is human, otheres non-human, physical, or environmental. This paper's research question is what is the major factor for the road traffic accidents in Korea. Analysis technique adopted for this paper is LISREL program. To compare in human and non-human factors which one is bigger, coefficients of gamma matrices were used. The coefficient of KSI 1, which explains human factor's effect, is 0.925. And the coefficient of KSI 2, which explains non-human factor's effect, 1.085. Notwithstanding the problems in data this program used, non-human factor's effect on traffic accidents is more powerful than human factor's effect in Korea.