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대구시(大邱市) 도축장(屠畜場)에서 처리(處理)된 돼지의 Salmonella 속균(屬菌)의 보균상태(保菌狀態)
탁련빈,Tak, Ryunbin 대한수의학회 1978 大韓獸醫學會誌 Vol.18 No.1
Ten strains of Salmonella were isolated from feces and lymph nodes of swine slaughtered at Daegu slaughter-house and the rate of isolation was 6.0 percent. Nine strains of Salmonella were isolated by enrichment in selenite F broth and one strain by direct culture on SS agar, but none of Salmonella were isolated from MacConkey ager and in SS broth. Among Salmonella isolated, Salmonella typhimurium occupied over half (6 strains) and the importance of Salmonella in swine for the incidence of food poisoning in man was discussed.
계유내(鷄由來) Escherichia coli의 항생물질내성(抗生物質耐性)및 R 인자(因子)의 분포(分布)
탁련빈,Tak, Ryunbin 대한수의학회 1977 大韓獸醫學會誌 Vol.17 No.1
Two hundred and twenty-seven strains of Escherichia coli isolated from 25 hens (12 hens received tetracycline neomycin and sulfadimethoxine, and 13 hens not received antibiotics) were studied for the drug resistance and distribution of R factors. About 74 per cent of E. coli strains isolated from hens of a herd received antibiotics were resistance to tetracycline (TC) streptomycin (SM), chloramphenicol (CM), kanamycin (KM), ampicillin (AP) and sulfisomidine (Su), alone or in combination thereof, but only a hen among a herd not received antibiotics excreted E. coli resistant to TC and SM. Among resistant strains, about 7% were found to be resistant to TC and SM, whereas 93% were resistant to three or more antibiotics. The most common pattern was the quadruple resistant to SM, TC, KM and Su (28.7%), and followed by triple ones to SM, TC and Su (25.3%), and SM, TC and KM (24.7%). About 84% of resistant strains carried R factors which were transferable to the recipient by conjugation.
Ryunbin Tak(卓鍊斌) 한국예방수의학회 1982 예방수의학회지 Vol.6 No.2
The distribution of salmonellae in a variety of zoo animals including carnivores, primates, ruminants and avifauna etc. was investigated. Out of the 203 samples collected from Dalsung Park in Taegu, only 2 yielded Salmonella typhimurium from one lion and one Korean raccoon dog.
Ryunbin Tak(卓練斌) 한국예방수의학회 1983 예방수의학회지 Vol.7 No.2
Microbial population of raw milk samples collected at Taegu area were enumerated by standard plate counts (SPC) and coliform counts, and 320 strains of coliform were studied the drug resistance and distribution of R factors. Total viable cells were from 1,921,000 to 9,933,000 and coliforms wete from 36,000 to 666,000 per mililiter of raw milk. In July the raw milk has highest SPC’s 22,451,000/ml and In January it has lowest SPC’s 93,000/ml. About 43 per cent of coliform strains isolated from raw milk were resistant to tetracycline (TC), streptomycine (SM), chloramphenicol (CM), ampicillin (AP) and kanamycine, alone or in combination thereof. Among the multiple resistant strains, the most common pattern was double resistant to SM and TC (14.6%), and followed by SM and CM(8.0%), and SM and AP (7.3%). About 28% of resistant strains carried R factors which were transferable to the recipient by conjugation.
Ryunbin Tak(卓練斌),Cheong Kyu Park(朴淸圭) 한국예방수의학회 1978 예방수의학회지 Vol.2 No.1
This study was carried out in order to know the distribution of Vibrio parahaemolyticus in marine specimens collected at the fishmarket in Taegu during August 1977. The rate of isolation of V. parahaemolyticus from fish and shellfish was 16.7 percent, and this organism was isolated more frequently from shellfish than from fish. Thirty-nine isolates were serotyped with K antisera from K1 through K57, and twenty. one strains were classified into 12 K types and 18 strains were nontypable. Of typed strains, K3, K11 and K57 were dominant. About one third of isolates was Kanagawa positive.
慶北地方 乳牛乳房炎의 疫學的 調査 및 治療對策에 關한 硏究
Ryunbin Tak(卓練斌),Young Hong Kim(金永洪),Hwa Sik Kim(金和植) 한국예방수의학회 1980 예방수의학회지 Vol.4 No.1
A total of 920 dairy cattle were examined for mastitis by California mastitis test (CMT) method. Milk samples from cattle infected with mastitis were studied bacteriologically and, among isolates, streptococci and staphylococci further examined for susceptibility to various antibiotics. The results obtained were as followings: Four hundred and seventy eight (52.0%) of 920 dairy cattle and 765(21.0%) of 3,642 quarters were positive for mastitis by CMT. It was also found that 395(85.7%) of the infected cattle were subclinical mastitis. A total of 261 strains of etiological agent were isolated from 254 quarters which showed CMT score (艹) or more. The important causative organisms were found to be Streptococcus agalactiae (34.1%) and Staphylococcus aureus (22.6%), and other streptococcus, S. epidermidis, coliform, pseudomonas, corynebacterium and yeast were also occasionally found to be causative agents. One hundred and forty five strains of streptococcus and 71 strains of staphylococcus were further examined for susceptibility to 9 antibiotics. Majority of strains were sensitive to kanamycin and leucomycin, and resistant to colistin, penicillin and streptomycin.
대구신천(大邱新川)으로부터 분리(分離)된 약제내성대장균군(藥劑耐性大腸菌群)의 전달성내성(傳達性耐性)에 관(關)하여
김기석,탁련빈,Kim, Ki Seuk,Tak, Ryunbin 대한수의학회 1977 大韓獸醫學會誌 Vol.17 No.2
The distribution of coliform population and drug-resistant coliforms in Shinchun water during April to August in 1976 and R factors in resistant strains were studied. The mean coliform population in the stream water was 361 organisms per 0.1ml and 53.5% of total coliforms were fecal coliforms. Thritynine and a half percent of total coliforms were streptomycin (SM) resistant to one or more of following antibiotics; tetracycline (TC), chloramphenicol (CM), ampicillin (AP) and kanamycin (KM). The most frequent resistant pattern was triple resistance to SM, TC and AP (23.8%) and followed by quadruple one to SM, TC, CM and AP (17.5%). About 87 percent strains carried R factors which were transferable by conjugation to E. coli ML 1410 or S. typhimurium LT 2.
비육용 홀스타인종 송아지에 발생한 Salmonella 감염증
이차수,탁련빈,Lee Cha Soo,Tak Ryunbin 대한수의사회 1979 대한수의사회지 Vol.15 No.9
At a farm raising 21 Holstein calves for meat production, 6 calves had died for 21 days with symptoms of diarrhea, inappetence, depression, dehydration and temporary rise in body temperature. Among died calves, 3 were tested pathologically and bacteriolog
Yersinia enterocolitica의 增菌培地에 관하여
Hoo Yeol Park(朴吼烈),Ryunbin Tak(卓練斌) 한국예방수의학회 1983 예방수의학회지 Vol.7 No.2
The enrichment media are necessary to euhance the growth of desirable organisms and to supress the growth of undesirable bacteria in specimens. Phosphate-buffered solution has been used for enrichment medium to isolate Yersinia enterocolitica, but it needs too long period of incubation to enrich this organism in specimen. Salmonella-Shigella (SS) agar has been used as selective or differential medium for enteric pathogens. Since this medium contains components which inhibit the growth of coliforms, it is reasonable to suppose that the formula of SS agar is effective for enrichment of Yersinia in feces. SS broth and MacConkey broth were prepared by filtering out the agar in solubilized SS agar and MacConkey agar, and the multiplication of Yersinia and E. coli in these media were tested at 15℃, 21℃ or 37℃. The growth of Yersinia in SS broth and MacConeky broth were superior to E. coli until 24 hours at 15℃, but the growth was not sufficient to isolate. At 21℃ of growth temperature, while the growth of Yersinia in MacConkey broth was inferior to E. coli, the enrichment of Yersinia in SS broth was very superior to E. coli as well as in SS broth contained 10 percent of swine feces for 18 and/or 24 hours.
닭에서 分離한 Escherichia coli의 重金屬 및 抗生物質耐性에 關하여
Yu Zu Chung(鄭有珠),Ryunbin Tak(卓鍊斌) 한국예방수의학회 1981 예방수의학회지 Vol.5 No.1
Escherichia coli isolated from chicken were studied for their resistance to heavy metals and antibiotics, and the relationship between the resistance to heavy-metals and antibiotics were evaluated. Resistance to arsenic(As), lead(Pb) and mercury(Hg) were found in 89.0, 71.4 and 16.5%, respectively, whereas all strains were sensitive to cadmium. Cultures with resistance to two metals (double) were isolated most frequently, followed by those resistant to one metal (single) and then those resistant to three (triple). The frequencies of resistance to tetracycline, streptomycin, chloramphenicol, ampicillin and kanamycin were 96.9, 70.9, 29.5, 23.9 and 5.3%, respectively. About 81% of multiple heavy-methal resistant strains were also resistant to 2 or more antibiotics. Among the above, 37 mercury and antibiotics resistant isolates were tested for their ability to transfer the restisance to susceptible strains of E. coli ML 1410. R plasmid carrying mercury resistance were demonstrated in 78% of the mercury- resistant strains of E. coli and almost antibiotics resistance were transfered simultaneously.