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RISS 인기검색어
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- 저자 : Ruiyi (검색결과 2 건)
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Gene expression and promoter methylation of porcine uncoupling protein 3 gene
Ruiyi Lin,Weimin Lin,Qiaohui Chen,Jianchao Huo,Yuping Hu,Junxiao Ye,Jingya Xu,Tianfang Xiao 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.2
Objective: Uncoupling protein 3 gene (UCP3) is a candidate gene associated with the meat quality of pigs. The aim of this study was to explore the regulation mechanism of UCP3 expression and provide a theoretical basis for the research of the function of porcine UCP3 gene in meat quality. Methods: Bisulfite sequencing polymerase chain reaction (PCR) and quantitative real-time PCR (Q-PCR) were used to analyze the methylation of UCP3 5′-flanking region and UCP3 mRNA expression in the adipose tissue or skeletal muscle of three pig breeds at different ages (1, 90, 210-day-old Putian Black pig; 90-day-old Duroc; and 90-day-old Dupu). Results: Results showed that two cytosine-guanine dinucleotide (CpG) islands are present in the promoter region of porcine UCP3 gene. The second CpG island located in the core promoter region contained 9 CpG sites. The methylation level of CpG island 2 was lower in the adipose tissue and skeletal muscle of 90-day-old Putian Black pigs compared with 1-day-old and 210-day-old Putian Black pigs, and the difference also existed in the skeletal muscle among the three 90-day-old pig breeds. Furthermore, the obvious changing difference of UCP3 mRNA expression was observed in the skeletal muscle of different groups. However, the difference of methylation status and expression level of UCP3 gene was not significant in the adipose tissue. Conclusion: Our data indicate that UCP3 mRNA expression level was associated with the methylation status of UCP3 promoter in the skeletal muscle of pigs.
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Yunsheng Xin,Ruiyi Dong 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.1
Rail defects not only cause crane structure damage but also lead to occupational disorders for the hoistman. To study the influence of rail defects on hoistman comfort, this study establishes a 7 degrees-of-freedom system dynamics model of a human body coupled with a crane-rail model on the basis of human biodynamics and Lagrange equations. Aiming at the problem of fatigue damage for human parts, this study proposes a dynamic response and analysis method for various parts of the human body on the basis of precise integration. Combined with crane optimization parameters, the vibration response of various parts of the human body is solved using the precise integration method. The calculation results are compared with the existing fatigue damage standards of various parts of the human body. Moreover, the damage situation and vibration comfort of various parts of the human body are analyzed, and the influence of seat parameters and nonlinear factors on the vibration of various parts of the human body is discussed. Results show that the comfort of various parts of the human body is poor at low speed and that the fatigue degree of various parts of the human body increases sharply with the increase of rail defects. The damping and stiffness of seats exert little influence on the force of each part of the human body. From the perspective of bearing force, no risk of injury to all parts of the human body exists. The two calculation results of the acceleration response of each part of the human body show minimal deviation under the linear and nonlinear models.
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