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RISS 인기검색어
- 검색키워드
- 저자 : Rongjin Liu (검색결과 4 건)
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Luo Rongjin,Li Gaocai,Zhang Weifei,Liang Huaizhen,Lu Saideng,Cheung Jason Pui Yin,Zhang Teng,Tu Ji,Liu Hui,Liao Zhiwei,Ke Wencan,Wang Bingjin,Song Yu,Yang Cao 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-
Both O-linked β-N-acetylglucosaminylation (O-GlcNAcylation) and endoplasmic reticulum-phagy (ER-phagy) are well-characterized conserved adaptive regulatory mechanisms that maintain cellular homeostasis and function in response to various stress conditions. Abnormalities in O-GlcNAcylation and ER-phagy have been documented in a wide variety of human pathologies. However, whether O-GlcNAcylation or ER-phagy is involved in the pathogenesis of intervertebral disc degeneration (IDD) is largely unknown. In this study, we investigated the function of O-GlcNAcylation and ER-phagy and the related underlying mechanisms in IDD. We found that the expression profiles of O-GlcNAcylation and O-GlcNAc transferase (OGT) were notably increased in degenerated NP tissues and nutrient-deprived nucleus pulposus (NP) cells. By modulating the O-GlcNAc level through genetic manipulation and specific pharmacological intervention, we revealed that increasing O-GlcNAcylation abundance substantially enhanced cell function and facilitated cell survival under nutrient deprivation (ND) conditions. Moreover, FAM134B-mediated ER-phagy activation was regulated by O-GlcNAcylation, and suppression of ER-phagy by FAM134B knockdown considerably counteracted the protective effects of amplified O-GlcNAcylation. Mechanistically, FAM134B was determined to be a potential target of OGT, and O-GlcNAcylation of FAM134B notably reduced FAM134B ubiquitination-mediated degradation. Correspondingly, the protection conferred by modulating O-GlcNAcylation homeostasis was verified in a rat IDD model. Our data demonstrated that OGT directly associates with and stabilizes FAM134B and subsequently enhances FAM134B-mediated ER-phagy to enhance the adaptive capability of cells in response to nutrient deficiency. These findings may provide a new option for O-GlcNAcylation-based therapeutics in IDD prevention.
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Zhang Weifeng,Li Gaocai,Luo Rongjin,Lei Jie,Song Yu,Wang Bingjin,Ma Liang,Liao Zhiwei,Ke Wencan,Liu Hui,Hua Wenbin,Zhao Kangcheng,Feng Xiaobo,Wu Xinghuo,Zhang Yukun,Wang Kun,Yang Cao 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-
Low back pain (LBP) is a major musculoskeletal disorder and the socioeconomic problem with a high prevalence that mainly involves intervertebral disc (IVD) degeneration, characterized by progressive nucleus pulposus (NP) cell death and the development of an inflammatory microenvironment in NP tissue. Excessively accumulated cytosolic DNA acts as a damage-associated molecular pattern (DAMP) that is monitored by the cGAS-STING axis to trigger the immune response in many degenerative diseases. NLRP3 inflammasome-dependent pyroptosis is a type of inflammatory programmed death that promotes a chronic inflammatory response and tissue degeneration. However, the relationship between the cGAS-STING axis and NLRP3 inflammasome-induced pyroptosis in the pathogenesis of IVD degeneration remains unclear. Here, we used magnetic resonance imaging (MRI) and histopathology to demonstrate that cGAS, STING, and NLRP3 are associated with the degree of IVD degeneration. Oxidative stress induced cGAS-STING axis activation and NLRP3 inflammasome-mediated pyroptosis in a STING-dependent manner in human NP cells. Interestingly, the canonical morphological and functional characteristics of mitochondrial permeability transition pore (mPTP) opening with the cytosolic escape of mitochondrial DNA (mtDNA) were observed in human NP cells under oxidative stress. Furthermore, the administration of a specific pharmacological inhibitor of mPTP and self-mtDNA cytosolic leakage effectively reduced NLRP3 inflammasome-mediated pyroptotic NP cell death and microenvironmental inflammation in vitro and degenerative progression in a rat disc needle puncture model. Collectively, these data highlight the critical roles of the cGAS-STING-NLRP3 axis and pyroptosis in the progression of IVD degeneration and provide promising therapeutic approaches for discogenic LBP.
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The technology and properties of sponge city permeable bricks prepared using refractory waste
Jieguang Song,Xueqing Yang,Ping Chen,Rongjin Liu,Deping Luo,Yuxuan Wei,Wenjin Yao,Jingjing Liu,Qing Zeng 한양대학교 세라믹연구소 2021 Journal of Ceramic Processing Research Vol.22 No.4
With the widespread development of sponge city projects across the country, the use of industrial permeable bricks hasincreased dramatically. My country produces a large amount of refractory waste and pollutes the environment seriously. Wasterefractory insulation bricks are used as the main raw materials, and a small amount of auxiliary raw materials are added toprepare sponge urban permeable bricks through forming and sintering processes. Through performance tests, such asporosity, flexural strength, and water permeability coefficient, the effect of process parameters on the performance ofpermeable bricks is studied, the process parameters of using refractory waste are comprehensively optimized to preparesponge urban permeable bricks, and permeable bricks are prepared for the industrialization of high-quality industrial solidwaste. Brick provides reference. Through the experimental results and analysis, the following conclusions are drawn: the rawmaterial ratio of refractory brick waste: binder: foaming agent: sintering aid is 80:10:1:9. Slurry water mill foaming occurswhen the water to material ratio is 0.5, the ball milling speed is 80 r/min, and the ball milling time is 2 h; the foaming effectand molding performance are better. In the drying stage, drying at 45 oC for 12 h has the best molding effect. The sinteringsystem at 1,300 oC and holding time of 1 h has the best sintering performance. After optimizing the process, the porosity ofthe permeable bricks can reach 48.4%, the water permeability coefficient is 2.1×10^-2 cm/s, the national permeable brick hasA-level standard, and the compressive strength is 26.8 MPa.
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Jieguang Song,Xueqing Yang,Ping Chen,Hao Xu,Deping Luo,Rongjin Liu,Zhijun Lai 한양대학교 세라믹연구소 2022 Journal of Ceramic Processing Research Vol.23 No.4
The prepared permeable bricks using electric porcelain waste as the main raw material are investigated, which improves theutilization rate of electric porcelain waste, and realizes the organic unity of recycling resource and ecological environmentprotection. The effects of different raw material ratios on the permeability coefficient, apparent porosity, compressive strength,volume shrinkage rate and microstructure properties of electric porcelain waste permeable bricks are investigated. The electricporcelain waste with a ratio of over 85 wt.% have greatly improved the utilization rate of electric porcelain waste. The resultsare shown that the water-to-material ratio or foaming agent content are increased, the porosity and water permeability of theelectric porcelain waste permeable brick are increased, but the compressive strength of the electric porcelain waste permeablebrick is decreased. The 90 wt.% electric porcelain waste, the 3 wt.% kaolin, the 5 wt.% sintering aid, and the 2 wt.% foamingagents are optimum raw materials ratios, the water-to-material ratio for 0.9 and the drying temperature at 40 oC, the sinteringtemperature at 1200 oC and holding time for 1 h are obtained the excellent performance electric porcelain waste permeablebricks. The permeability coefficient is 3.9×10−2 cm/s, the volume shrinkage rate is 37.88%, the compressive strength is 1.146MPa, and the porosity is 79.0%.
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