http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Acute and Repeated 28-Day Oral Dose Toxicity Studies of Thymus vulgaris L. Essential Oil in Rats
Rojas-Armas, Juan,Arroyo-Acevedo, Jorge,Ortiz-Sanchez, Manuel,Palomino-Pacheco, Miriam,Castro-Luna, Americo,Ramos-Cevallos, Norma,Justil-Guerrero, Hugo,Hilario-Vargas, Julio,Herrera-Calderon, Oscar Korean Society of ToxicologyKorea Environmental Mu 2019 Toxicological Research Vol.35 No.3
Thymus vulgaris L. is widely used as an ingredient in cooking and in herbal medicine. However, there is little information about its toxicity. The present study was performed to evaluate the acute and repeated 28-day oral dose toxicity of thyme essential oil in rats. For the acute toxicity test, two groups of three rats were used. The rats received a single dose of essential oil: 300 or 2,000 mg/kg of body weight (bw). The rats were observed individually during the first four hours, and then daily until day 14. For the toxicity test with repeated doses, four groups of 10 rats were used. Doses of 100, 250, and 500 mg/kg/day were tested for 28 days. At the end of the experiment, blood was collected and the animals were sacrificed. Histopathological examination showed that in the lungs of rats given the 2,000 mg/kg bw dose, polymorph nuclear infiltrates, hemosiderin macrophages, and interstitial space thickening were present. In the repeated dose study, all rats survived the 28-day treatment period and apparently showed no signs of toxicity. The hematological and biochemical parameters were not altered. The histopathological study of the organs showed severe changes in the lung, with the dose of 500 mg/kg/day; in the other organs, no alterations were observed or the changes were slight. The body weight was only altered in male rats given the 500 mg/kg dose. The relative weight of the organs did not show any significant changes. Our studies revealed that the essential oil of Thymus vulgaris has moderate oral toxicity according to the results of the acute test, whereas the results of the 28-day oral toxicity test suggest that the no-observed-adverse effect level (NOAEL) is greater than 250 mg/kg/day.
Rutin Prevents LTA Induced Oxidative Changes in H9c2 Cells
Gloria Gutierrez-Venegas,Berenice Fernandez-Rojas,Marisol Rosas-Martinez,Manuel Alejandro Sanchez-Carba 한국식품영양과학회 2020 Preventive Nutrition and Food Science Vol.25 No.2
Lipoteichoic acid (LTA), a component of Gram-positive bacteria cell walls is involved in infective endocarditis (IE), a life-threatening disease. We evaluated for the first time, whether flavonoid rutin (quercetin-3-rutinoside) can block LTA-induced pro-inflammatory response and reactive oxygen species (ROS) production, and reduction of antioxidant enzymes. We found that rutin suppresses LTA effects on the antioxidant enzymes superoxide dismutase, catalase, and glutathione peroxidase, as well as the pro-inflammatory enzyme cyclooxygenase-2, preventing phosphorylation of the mitogen-activated protein kinases (MAPKs), p38, and c-Jun N-terminal kinase, and the increase of ROS production induced by LTA. Taken together, these findings suggest that rutin prevents oxidative damage, inflammation, and MAPKs activation induced by LTA. Rutin may exert a protective effect in IE. These data provide novel insights for future use of rutin to prevent the mechanisms of LTA-related pathogenesis, inflammatory processes, and antioxidant enzyme levels in diseases such as IE.
On Trapping Porphyrin Free-Bases Between Graphene Oxide Plates
Carlos J. Montes,María Luisa Ojeda,Federico Gonz alez,Miguel Angel García-Sanchez,Fernando Rojas,Celso Velasquez 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2015 NANO Vol.10 No.4
A synergistic combination of properties between graphene oxide (GO) nanosheets and porphyrin molecules has been accomplished via the entrapment of the latter species within the pore space formed by parallel plates of the carbon material. A hybrid material, synthesized in this manner, would likely lead to the development of efficient photovoltaic cells, given the high capacity of porphyrin molecules to absorb sunlight or radiation of wavelength shorter than 700 nm as well as because of the excellent electron conduction and fine solar energy capture that can be achieved by the graphene substrate. Herein, materials consisting of trapped porphyrin molecules in-between GO plates have been prepared to develop preliminary solar cell devices for an efficient capture of sunlight energy. The hybrid systems created this way can be physicochemically characterized through diverse techniques, which include fluorescence emission to confirm and avail both the presence and manifest luminescent characteristics of the tetrapyrrolic species that are captured and retained in-between GO plates.
Franklin Hitler Fernandez-Zarate,Annick Estefany Huaccha-Castillo,Lenin Qui~nones-Huatangari,Segundo Primitivo Vaca-Marquina,Tito Sanchez-Santillan,Eli Morales-Rojas,Alejandro Seminario-Cunya,Marly Gu 한국산림과학회 2022 Forest Science And Technology Vol.18 No.4
Cinchona officinalis, known locally as cascarilla or cinchona, is a plant species native to SouthAmerica. It was used as a source of quinine to combat malaria in the 17th century. The spe-cies is threatened by various anthropogenic activities. Further, the propagation of the spe-cies depends on seed dispersal and its germination capacity. Therefore, it is necessary toconserve and propagate this species. BecauseC. officinalisseeds have a low germinationcapacity, we determined the effect of arbuscular mycorrhizae (AM) on their germination andgrowth. A randomized design was employed with two treatments, one treated with mycor-rhizae (CM) and another without mycorrhizae (SM). For each treatment, three replicates of100 seeds were used. Germination, growth, and fungal characteristics were evaluated. In ger-mination parameters, the CM treatment showed better performance, but the improvementwas statistically insignificant. However, the application of AM significantly improved seedlingheight (cm), root length (cm), leaf area (cm2), and root number by 53.52, 28.72, 29.73, and61.66%, respectively. Likewise, mycorrhization intensity (%), mycorrhization frequency (%),and extraradical mycelium length (cm) in the CM treatment were 37.13, 3.44, and 174.97%higher compared to the SM treatment, respectively. Therefore, the use of AM fungi provesto be advantageous in the propagation ofC. officinalis, and these results provide a basis forthe largescale and sustainable propagation of this species.
Huaccha-Castillo Annick Estefany,Fernandez-Zarate Franklin Hitler,Pérez-Delgado Luis Jhoseph,Tantalean-Osores Karla Saith,Vaca-Marquina Segundo Primitivo,Sanchez-Santillan Tito,Morales-Rojas Eli,Semin 한국산림과학회 2023 Forest Science And Technology Vol.19 No.1
Non-destructive methods that accurately estimate leaf area (LA) and leaf weight (LW) are simple and inexpensive, and represent powerful tools in the development of physiological and agronomic research. The objective of this research is to generate mathematical models for estimating the LA and LW of Cinchona officinalis leaves. A total of 220 leaves were collected from C. officinalis plants 10months after transplantation. Each leaf was measured for length, width, weight, and leaf area. Data for 80% of leaves were used to form the training set, and data for the remaining 20% were used as the validation set. The training set was used for model fit and choice, whereas the validation set al.lowed assessment of the of the model’s predictive ability. The LA and LW were modeled using seven linear regression models based on the length (L) and width (Wi) of leaves. In addition, the models were assessed based on calculation of the following statistics: goodness of fit (R2), root mean squared error (RMSE), Akaike’s information criterion (AIC), and the deviation between the regression line of the observed versus expected values and the reference line, determined by the area between these lines (ABL). For LA estimation, the model LA¼11.521(Wi) 21.422 (R2¼0.96, RMSE¼28.16, AIC¼3.48, and ABL¼140.34) was chosen, while for LW determination, LW¼0.2419(Wi) 0.4936 (R2¼0.93, RMSE¼0.56, AIC¼37.36, and ABL¼0.03) was selected. Finally, the LA and LW of C. officinalis could be estimated through linear regression involving leaf width, proving to be a simple and accurate tool.