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        Assessing the adaptability and stability of new pearl millet hybrids for grain yield, grain iron and zinc content in Ghana using AMMI analysis

        Asungre Peter Anabire,Akromah Richard,Kena Alexander Wireko,Gangashetty Prakash 한국작물학회 2022 Journal of crop science and biotechnology Vol.25 No.5

        Understanding the role of environment, genotype and their interaction effect on the estimation of magnitude of variability and heritability of yield traits and micronutrients (grain Fe and Zn) content to facilitate the introduction of high yielding hybrids in Ghana is an important requirement. Twenty-four pearl millet (Pennisetum glaucum (L) R. Br) single cross hybrids were evaluated at four environments of northern Ghana during the 2018 and 2019 cropping seasons. Additive Main Effects and Multiplicative Interaction (AMMI) model elucidated extent of genotype x environment (GxE) interaction, and stability across the environments. Significant difference (p < 0.001) was observed among the hybrids and test environments. Genotypic variance (σ2G) contributed a greater proportion of the phenotypic variance (σ2p) for four traits including grain Fe content and yield, whiles GxE interaction (σ2gxe) variance accounted for greater proportion of the σ2p for five traits namely downy mildew incidence, days to 50% flowering, panicle girth, 1000-seed weight and grain Zn content. This shows both additive and non-additive gene action in inheritance of these traits. Moderate to high broad-sense heritability was recorded for most traits, especially grain yield and grain micronutrient, implying significant trait improvement may be achieved through recurrent selection. Overall, 3 hybrids viz ICMH IS 16013, ICMH 177023 and ICMH IS 16015 combined high yield with stability.

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        In-vitro Regeneration of Interspecific Hybrids in Eggplant Species via Seed and Embryo Culture

        Nusrat Tsemah Afful,Daniel Nyadanu,Richard Akromah,Harry Mensah Amoatey,Fuseini Mohammed,Clement Annor 한국육종학회 2020 Plant Breeding and Biotechnology Vol.8 No.3

        Interspecific hybrids of eggplants (Salanum species) are of prime concern to breeders, as they allow introgression of useful genes from wild species to cultivated ones. However, successful hybridization between species is usually very low. This hampers development of improved hybrids. In-vitro culture techniques may be used to overcome interspecific barriers. Seeds and excised embryos obtained from two crosses (Solanum (S.) melongena × S. torvum and S. melongena × S. anguivi) were inoculated on Murashige and Skoog (MS) basal medium supplemented with varying concentrations of 3-indoleacetic acid (IAA) and 6-benzylaminopurine (BAP). Addition of hormones in the culture medium significantly (P < 0.05) improved root and shoot emergence, seed germination and seedling survival. However, excised embryos cultured on the same medium did not develop. Hybrid seeds cultured on MS medium supplemented with different concentrations of IAA and BAP germinated within 6-14 days (SM001-07 × ST004-03) and 4-10 days (SM001-07 × San005-01). Optimal concentration of IAA and BAP for seed germination and seedling survival depended on hybrid type. Regenerated plantlets were successfully transferred to the field after initial acclimatization. High levels of heterosis observed for number of seeds/fruit and fruit breadth suggested an opportunity for developing F1 hybrids with desirable fruit characters for improved fruit yield.

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        Phenotypic characterization of taro [Colocasia esculenta (l.) Schott.] accessions for yield and nutrient composition in Ghana

        Donkor Esther Fobi,Nyadanu Daniel,Akromah Richard,Osei Kingsley 한국작물학회 2023 Journal of crop science and biotechnology Vol.26 No.3

        Phenotypic characterization is a pre-requisite activity for any breeding program as it reveals the available diversity in the existing germplasm for parental selection. The objective of the study was to evaluate and characterize local and introduced germplasm of taro [Colocasia esculenta (l.) Schott.] for yield and nutritional quality in Ghana. Thirty-four (34) taro accessions were cultivated at research feld of Crop Research Institute—Council for Scientifc and Industrial Research (CRI-CSRI), Fumesua in the Ashanti Region of Ghana. Randomized Complete Block Design with three replications was used for the research. Data on leaf length and width (cm), number of inforescence/plant, number of leaves, number of stolons, number of suckers, plant height (cm), plant span (cm), corm length, corm weight (cm), yield(kg/ha) and percentage dry matter were measured. The corms were also analyzed for their nutritional composition according to AOAC (1990). Data collected were subjected to Analysis of Variance, Shannon–Weaver diversity test, correlation test and multivariate analysis. Signifcant variation (p<0.05) was observed for the yield and yield related traits and the nutritional traits. The principal component analysis revealed corm weight (g) and yield (kg/ha) for yield traits and iron and zinc for the nutrient composition as the most important traits. The cluster analysis grouped the accessions into three clusters based on both the yield and nutritional traits. There were both signifcant positive and negative correlation between the yield and yield traits and also the nutritional traits. Accessions BL/SM/10, BL/SM/151, CE/MAL/32, KA 019 and CE/IND/16 were identifed as potential parental lines for yield and nutritional improvement of taro in Ghana.

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