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- 저자 : Daniel Nyadanu (검색결과 3 건)
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In-vitro Regeneration of Interspecific Hybrids in Eggplant Species via Seed and Embryo Culture
Nusrat Tsemah Afful,Daniel Nyadanu,Richard Akromah,Harry Mensah Amoatey,Fuseini Mohammed,Clement Annor 한국육종학회 2020 Plant Breeding and Biotechnology Vol.8 No.3
Interspecific hybrids of eggplants (Salanum species) are of prime concern to breeders, as they allow introgression of useful genes from wild species to cultivated ones. However, successful hybridization between species is usually very low. This hampers development of improved hybrids. In-vitro culture techniques may be used to overcome interspecific barriers. Seeds and excised embryos obtained from two crosses (Solanum (S.) melongena × S. torvum and S. melongena × S. anguivi) were inoculated on Murashige and Skoog (MS) basal medium supplemented with varying concentrations of 3-indoleacetic acid (IAA) and 6-benzylaminopurine (BAP). Addition of hormones in the culture medium significantly (P < 0.05) improved root and shoot emergence, seed germination and seedling survival. However, excised embryos cultured on the same medium did not develop. Hybrid seeds cultured on MS medium supplemented with different concentrations of IAA and BAP germinated within 6-14 days (SM001-07 × ST004-03) and 4-10 days (SM001-07 × San005-01). Optimal concentration of IAA and BAP for seed germination and seedling survival depended on hybrid type. Regenerated plantlets were successfully transferred to the field after initial acclimatization. High levels of heterosis observed for number of seeds/fruit and fruit breadth suggested an opportunity for developing F1 hybrids with desirable fruit characters for improved fruit yield.
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Donkor Esther Fobi,Nyadanu Daniel,Akromah Richard,Osei Kingsley 한국작물학회 2023 Journal of crop science and biotechnology Vol.26 No.3
Phenotypic characterization is a pre-requisite activity for any breeding program as it reveals the available diversity in the existing germplasm for parental selection. The objective of the study was to evaluate and characterize local and introduced germplasm of taro [Colocasia esculenta (l.) Schott.] for yield and nutritional quality in Ghana. Thirty-four (34) taro accessions were cultivated at research feld of Crop Research Institute—Council for Scientifc and Industrial Research (CRI-CSRI), Fumesua in the Ashanti Region of Ghana. Randomized Complete Block Design with three replications was used for the research. Data on leaf length and width (cm), number of inforescence/plant, number of leaves, number of stolons, number of suckers, plant height (cm), plant span (cm), corm length, corm weight (cm), yield(kg/ha) and percentage dry matter were measured. The corms were also analyzed for their nutritional composition according to AOAC (1990). Data collected were subjected to Analysis of Variance, Shannon–Weaver diversity test, correlation test and multivariate analysis. Signifcant variation (p<0.05) was observed for the yield and yield related traits and the nutritional traits. The principal component analysis revealed corm weight (g) and yield (kg/ha) for yield traits and iron and zinc for the nutrient composition as the most important traits. The cluster analysis grouped the accessions into three clusters based on both the yield and nutritional traits. There were both signifcant positive and negative correlation between the yield and yield traits and also the nutritional traits. Accessions BL/SM/10, BL/SM/151, CE/MAL/32, KA 019 and CE/IND/16 were identifed as potential parental lines for yield and nutritional improvement of taro in Ghana.
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Akpertey Abraham,Anim-Kwapong Esther,Adu-Gyamfi Paul Kwasi Krah,Dadzie Abu Mustapha,Nyadanu Daniel,Ofori Atta 한국작물학회 2023 Journal of crop science and biotechnology Vol.26 No.2
Agronomic performance of Robusta cofee (Cofea canephora) is afected by genotype by environment interaction, which demands multi-environment testing of genotypes to identify superior genotypes. The objectives of this study were to (1) estimate genetic parameters and analyze the efects of genotype (G), environment (E), and genotype by environment (G×E) interaction on growth and yield traits of C. canephora genotypes, (2) identify superior genotypes that exhibit high stability by combining high growth and yield with broad or specifc environmental adaptation, and (3) identify environments that best represent the target environment for high trait expression. Thirty-nine C. canephora hybrids were evaluated using a randomized-complete-block design with three replications at New Tafo-Akim and Bechem in the Eastern and Ahafo regions of Ghana, respectively. Additive main efect and multiplicative interaction (AMMI) analysis of variance revealed highly signifcant (p<0.001) diferences among G, E, and G×E interaction efects for all traits. AMMI2 biplot analysis revealed the presence of 3, 2, 3, 2, and 3 mega environments for trunk cross-sectional area, height, span, number of laterals, and yield, respectively. Identifying mega environments for these traits will help expedite Robusta cofee breeding through the reduction of the number of test environments needed for phenotype evaluations. The AMMI analyses indicated genotypes G17 (E138×C180), G35 (PA193×C180), G4 (197×PA413), G1 (149×C193) and G30 (E152×149) have high and stable yields across all test environments. The promising candidate genotypes are recommended for further stability tests and release in Ghana or comparable environments.
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