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Competence factor 에 의한 폐염균의 형질전환능 유도
이동권 성균관대학교 약학연구소 1989 成均藥硏論文集 Vol.1 No.1
Abstract-To facilitate competence monitoring and induction in Streptococcus pneumoniae, degradation of radioactive DNA and appearance of transformants(appearance of competence) were tested. Degradation of radioactive DNA in culture media conincided very well with the appearance of transformants. Therefore competence could be monitored by testing DNA degradation in culture media. To induce competence, a culture supernatant containing competence factor was harvested just prior to competence appearance and used for competence induction. When 0.1%(v/v) of the culture supernatant was added to a non-competent culture, competence was induced within 20 minutes.
Western Blot Analysis에 의한 폐염균 단백질의 검출
이동권 成均館大學校 科學技術硏究所 1989 論文集 Vol.40 No.1
A convenient and sensitive method for detection of a protein antigen from Streptococcus pneumoniae (pneumococcus) by Western blot analysis was developed. This method allows to determine nanogram range of protein antigen in the lysate of pneumococcus and does not require electroblotting apparatus nor cold room facilities. To facilitate protein transfer from polyacrylamide gel to nitrocellulose paper, ratio of bis-acrylamide/acrylamide was reduced to 1/100. After polyacrylamide gel electrophoresis, the proteins in the gel were transferred to nitrocellulose paper by blotting at room temperature just the same v, ay as the Southern blot transfer. A novel analog of E. coli RecA protein, molecular weight of 24 kilodalton, was identified from pneumococcus Mate by this method.
문은이,이동권,표석능 성균관대학교 약학연구소 1996 成均藥硏論文集 Vol.8 No.1
Aflatoxin B1 (AFB1) has been reported to directly suppress the immune responses. In the present study, the effect of AFB1 on immune functions was investigated. Splenic lymphocytes were treated with various doses of the mitogens (lipopolysaccharide, concanavalin A) in the presence of AFB1. AFB1 pretretment decreased the number of plaque forming cells (PFC) in a dose-dependent manner. Antibody production of IgM and IgG class was significantly decreased in AFB1-treated splenic cells. In addition, when animals were exposed to AFB1, the susceptibility of bacterial infection as well as the growth of tumor cells was increased. These data suggest that AFB1 affected the immune function and humoral immunity impaired by AFB1 treatment contributed to pathological process.
김수남,이동권,표석능 성균관대학교 약학연구소 1993 成均藥硏論文集 Vol.5 No.1
Streptococcus pneumoniae remains a major cause of bacteremia, meningitis, penumonia, sinusitis, and otitis media. Although this organism was originally exquisitely susceptible to penicillin, the last two decades have witnessed the emergence of strains resistant to penicillin as well as other antimicrobial agents in many parts of the world. 102 isolates obtained from patients with invasive pneumococcal disease have been examined the susceptibility to various antibiotics. 52% to 85% of isolates tested were resistant to various antibiotics. These data suggest that more powerful antibiotics should be used for the treatment of pneumococal infection.
민관기,표석능,이동권 성균관대학교 약학연구소 1999 成均藥硏論文集 Vol.11 No.-
Abstract-Streptococcus pneumoniae (pneumococcus) is the most frequent causative agent of acute bacterial pneumonia. Outstanding characteristic of pneumococcus is an ample polysaccharide capsule that is highly antigenic agent and is the major factor for classification of pneumococcus into more than 94 serotypes. In this study, production of capsular polysaccharide from Streptococcus pneumoniae type 23 was optimized by supplementation of metal ions or by modulation of culture conditions. When brain heart infusion (BHI) broth was supplemented with 1 mM concentration of CaCl_2, CuSO_4 and MgSO_4, growth of pneumococcus as well as polysaccharide yield were stimulated. Also slight aeration gave rise to better polysaccharide yield.
Instability of pneumococcus library in pHC79 and pAcyc184
Rhee, Dong-Kwon The Pharmaceutical Society of Korea 1995 Archives of Pharmacal Research Vol.18 No.1
S. pneumoniae (pneumococcus) gene cloning and library construction in E. coli multicopy plasmid has been hampered, in part, by instability problems. In this study, stability of pneumococcus gene library in cosmid vector and pACYC184 was examined. Pneumococcus library in the cosmid vector pHC79 was extermely unstable that most of the recobinant clones were degenerated rapidly. Only 2 out 849 clones were stable and had appropriate insert size. Pneumococcus library in pACYC184 was also so unstable that the pneumococcal inserg and/or part of the vector were deleted. However, the instability problems seemed to be resolved when transcription teminator plasmid was employed for pneumococcus library construction.
Dong-Kwon Rhee The Korean Society of Ginseng 2023 Journal of Ginseng Research Vol.47 No.2
Vaccines help protect people from infections. However, Coronavirus 2019 (COVID-19) vaccinees often still become infected with COVID-19 variants (breakthrough infections) and may go on to suffer from long COVID symptoms due to short-lasting immunity and less-effective protection provided by available vaccines. Moreover, the current COVID-19 vaccines do not prevent viral transmission and ward off only about 15% of breakthrough infections. To prepare more effective vaccines, it is essential to predict the viral strains that will be circulating based on available epidemiological data. The World Health Organization recommends in advance which influenza strains are expected to be prevalent during influenza season to guide the production of influenza vaccines by pharmaceutical companies. However, future emerging COVID-19 strain(s) have not been possible to predict since no sound epidemiological information has been established. Thus, for more effective protection, immune stimulators alone or in combination with vaccines would be preferable to protect people from COVID-19 infection. One of those remedies would be ginseng, which has been used for potentiating immunity in the past.